A5055446488- Pregnancy and preeclampsia studies
- MicroRNA in disease regulation
- Angiogenesis and VEGF in Cancer
- Cancer-related molecular mechanisms research
- Zebrafish Biomedical Research Applications
- Birth, Development, and Health
- Gestational Diabetes Research and Management
- Blood Coagulation and Thrombosis Mechanisms
- Neonatal Respiratory Health Research
- Neuropeptides and Animal Physiology
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Reproductive System and Pregnancy
- Pharmaceutical studies and practices
- Diabetes Treatment and Management
- Heart Failure Treatment and Management
- Mesenchymal stem cell research
- Nitric Oxide and Endothelin Effects
- Electrolyte and hormonal disorders
- Pharmacological Effects and Toxicity Studies
- Immune responses and vaccinations
Medical University of Graz
2019-2025
Endothelial dysfunction is associated with several lifestyle-related diseases, including cardiovascular and neurodegenerative it contributes significantly to the global health burden. Recent research indicates a link between risk factors (CVRFs), excessive production of reactive oxygen species (ROS), mitochondrial impairment, endothelial dysfunction. Circulating progenitor cells (EPCs) are recruited into vessel wall maintain appropriate function, repair, angiogenesis. After attachment, EPCs...
MicroRNAs (miRNAs) are regulators of mRNA translation and play crucial roles in various physiological pathological processes. In this study, we profiled miRNAs umbilical cord plasma (UCP) to explore the association neonatal circulating with maternal metabolic parameters anthropometric, inflammatory characteristics healthy pregnancies. Data UCP samples were collected from 16 pregnancies, equally divided between normal-weight overweight mothers male female newborns. Using next-generation...
Abstract Recent research has highlighted the significance of succinate and its receptor in gestational diabetes (GDM) pathogenesis. However, a clear interconnection between placenta metabolism, levels, SUCNR1 signalling pregnancy pathologies remains elusive. Here, we set out to investigate potential role on labour placental mechanisms by combining clinical functional experimental data at same time as exploring specific SUCNR1‐mediated effects vascularization, addressing agonist actions....
Function and dysfunction of endothelial cells are regulated by a multitude factors. Endothelial cell research often requires in vitro culture experiments. Hence, various media specifically designed to promote growth available. These strikingly differ their composition: complex contain supplement (ECGS), an extract produced bovine brain with undefined amounts biologically active compounds, whilst defined selected factors concentrations. We here compared the effect seven purchasable on colony...
Endothelial function and the risk for endothelial dysfunction differ between males females. Besides action of estrogen, sex chromosome gene expression programming effects also provoke this sexual dimorphism. MicroRNAs (miRNAs) have emerged as regulators cell dysfunction. We here hypothesized distinct miRNA patterns in male versus female human cells that contribute to functional differences. used our well-established model fetal isolated from placenta (fpEC) analyzed dimorphic potentially...
Maternal overweight in pregnancy alters the metabolic environment and generates chronic low-grade inflammation. This affects fetal development programs offspring’s health for developing cardiovascular disease later life. MME (membrane-metalloendopeptidase, neprilysin) cleaves various peptides regulating vascular tone. Endothelial cells express membrane-bound soluble MME. In adults, of obesity upregulates endothelial circulating We here hypothesized that maternal increases feto-placental...
Abstract Background Maternal cardiovascular risk factors (CVRF) in pregnancy, i.e., obesity and hyperglycemia, transmit to the fetus affect placental fetal endothelial function. Moreover, a sex dimorphism function susceptibility towards CVRF exists already utero . Endothelial colony-forming cells (ECFC) are circulating progenitors highly present neonatal cord blood sensitive CVRF. This study investigated whether or subtle maternal metabolic changes within healthy range alter ECFC outgrowth....
Inflammatory memory involves the molecular and cellular ‘reprogramming’ of innate immune cells following exogenous stimuli, leading to non-specific protection against subsequent pathogen exposure. This phenomenon has now also been described in non-hematopoietic cells, such as human fetal adult endothelial cells. In this study we mapped cell-specific DNA methylation profile transcriptomic remodelling during establishment inflammatory two distinct cell types – a progenitor (ECFC)...
Gestational diabetes (GDM) and preeclampsia (PE) are associated with fetal hyperglycemia, hypoxia, or both. These adverse conditions may compromise placental endothelial cells. In fact, GDM PE affect feto-placental function also program cardiovascular disease risk of the offspring in long-term. MicroRNAs short, non-coding RNAs that regulate protein translation fine tune biological processes. A group microRNAs termed angiomiRs is particularly involved regulation function. We hypothesized...
Abstract High gestational weight gain (GWG) is a cardiovascular risk factor and may disturb neonatal endothelial function. Long non‐coding RNAs (lncRNAs) regulate gene expression epigenetically can modulate Endothelial colony forming cells (ECFCs), circulating precursors, are recruitable source of sustain function, vascular growth repair. We here investigated whether higher GWG affects ECFC function elucidated the role lncRNAs herein. Wound healing umbilical cord blood‐derived ECFCs after...
Human umbilical vein and artery endothelial cells (HUVEC; HUAEC), placental (fpAEC), colony-forming (ECFC) from cord blood are a widely used model for researching vascular development, fetal function, the effect of adverse conditions in pregnancy thereon. However, development angiogenesis start first weeks gestation, may also affect function before term, suggesting that early respond differently. Thus, we established novel, gentle flow-through method to isolate pure human trimester (FTUEC)....
Abstract Disclosure: A. Kulovic-Sissawo: None. E. Weiss: Jantscher-Krenn: U. Hiden: Background: In the complex nature of pregnancy, female organism undergoes pivotal vascular and metabolic changes orchestrated by a tightly regulated interplay bioactive regulatory molecules. The neutral endopeptidase, neprilysin, exists both in membrane-bound soluble form (sNEP), with latter present circulatory system. Neprilysin degrades an exciting range peptides involved glycemic control (insulin B chain,...
Einleitung Neprilysin (Membran-Metalloendopeptidase, MME) ist eine Protease, die Reihe physiologisch relevanter, bioaktiver Peptide abbaut: spaltet u.a. Glukagon, glucagon-like peptide 1 (GLP1) und Resistin, daher möglicherweise auf komplexe Weise an der Blutzuckerregulation beteiligt. Tatsächlich verbessern Neprilysin-Inhibitoren Insulinempfindlichkeit [1]. liegt in einer membrangebundenen Form vor, deren aktives Zentrum dem extrazellulären Raum zugewandt ist. Durch Abspaltung wird es von...