A5042030738- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Wastewater Treatment and Nitrogen Removal
- Effects and risks of endocrine disrupting chemicals
- Heavy Metal Exposure and Toxicity
- Health, Environment, Cognitive Aging
- Microbial bioremediation and biosurfactants
- Microbial Fuel Cells and Bioremediation
- Toxic Organic Pollutants Impact
- Anaerobic Digestion and Biogas Production
- Metabolomics and Mass Spectrometry Studies
- Amino Acid Enzymes and Metabolism
- Biosimilars and Bioanalytical Methods
- Bacteriophages and microbial interactions
- Pregnancy and Medication Impact
- Environmental Justice and Health Disparities
- Birth, Development, and Health
- Bacillus and Francisella bacterial research
- Mycotoxins in Agriculture and Food
- Genetically Modified Organisms Research
- Photosynthetic Processes and Mechanisms
- Water Treatment and Disinfection
- Atmospheric and Environmental Gas Dynamics
- Advanced Proteomics Techniques and Applications
- Microbial metabolism and enzyme function
Northwestern University
2018-2024
University of Tennessee at Knoxville
2017-2019
Oak Ridge National Laboratory
2017-2019
ABSTRACT The anaerobic oxidation of methane by methanotrophic (ANME) archaea in syntrophic partnership with deltaproteobacterial sulfate-reducing bacteria (SRB) is the primary mechanism for removal ocean sediments. their syntrophy has been subject much research as traditional intermediate compounds, such hydrogen and formate, failed to decouple partners. Recent findings have indicated potential extracellular electron transfer from ANME SRB, though it unclear how electrons are integrated into...
Remediation of industrial wastewater is important for preventing environmental contamination and enabling water reuse. Biological treatment one contaminant, thiocyanate (SCN–), relies upon microbial hydrolysis, but this process sensitive to high loadings. To examine the activity stability a community over increasing SCN– loadings, we established operated continuous-flow bioreactor fed loadings SCN–. A second reactor was ammonium sulfate mimic breakdown products Biomass sampled from both...
Sulfate is the predominant electron acceptor for anaerobic oxidation of methane (AOM) in marine sediments. This process carried out by a syntrophic consortium methanotrophic archaea (ANME) and sulfate reducing bacteria (SRB) through an energy conservation mechanism that still poorly understood. It was previously hypothesized ANME alone could couple to dissimilatory reduction, but genetic biochemical basis this proposal has not been identified. Using comparative genomic phylogenetic analyses,...
Naturally produced and anthropogenically released DCM can reside in anoxic environments, yet little is known about the diversity of organisms, enzymes, mechanisms involved carbon-chlorine bond cleavage absence oxygen. A proteogenomic approach identified two RDases four corrinoid-dependent methyltransferases expressed by degrader “ Candidatus Dichloromethanomonas elyunquensis” strain RM, suggesting that reductive dechlorination methyl group transfer play roles anaerobic degradation. These...
To what extent multi-omic techniques could reflect in situ microbial process rates remains unclear, especially for highly diverse habitats like soils. Here, we performed microcosm incubations using sandy soil from an agricultural site Midwest USA. Microcosms amended with isotopically labeled ammonium and urea to simulate a fertilization event showed nitrification (up 4.1 ± 0.87 µg N-NO3- g-1 dry d-1) accumulation of N2O after 192 hours incubation. Nitrification activity (NH4+ → NH2OH NO NO2-...
The eurypsychrophilic bacterium Planococcus halocryophilus is capable of growth down to -15°C, making it ideal for studying adaptations subzero growth. To increase our understanding the mechanisms and pathways important growth, we performed proteomics on P. grown at 23°C, 23°C with 12% w/v NaCl -10°C NaCl. Many proteins increased abundances versus also 23C-salt indicating a closely tied relationship between salt cold stress adaptation. Processes which displayed largest changes in protein...
Multi-omic techniques can offer a comprehensive overview of microbial communities at the gene, transcript and protein levels. However, to what extent these levels reflect in situ process rates is less clear, especially highly complex habitats such as soils. Here we performed microcosm incubations using soil from site with history agricultural management. Microcosms, amended isotopically labelled ammonium urea simulate fertilization event, showed nitrification (up 4.1 ± 0.87 µg N-NO 3 - g -1...
Background: Environmental Influences on Child Health Outcomes (ECHO) is an NIH program linking 84 existing longitudinal cohorts of more than 50,000 children from across the US to understand effects environmental influences child health and development. ECHO represents unprecedented opportunity improve our understanding developmental chemical exposures during childhood their associations with a range adverse outcomes.Objective: We reviewed current exposure data available in provided framework...