A5102019789- HIV Research and Treatment
- Cellular transport and secretion
- Immune Cell Function and Interaction
- Virology and Viral Diseases
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Biotin and Related Studies
- Retinal Development and Disorders
- HIV/AIDS drug development and treatment
- Retinoids in leukemia and cellular processes
- Edible Oils Quality and Analysis
- Mosquito-borne diseases and control
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Genomic variations and chromosomal abnormalities
- Transgenic Plants and Applications
- Dye analysis and toxicity
- Mast cells and histamine
- Glycosylation and Glycoproteins Research
- Click Chemistry and Applications
- Folate and B Vitamins Research
- Cholesterol and Lipid Metabolism
- Phytochemical Studies and Bioactivities
- Liver physiology and pathology
University of Massachusetts Dartmouth
2017-2024
Waters (China)
2024
Waters (United States)
2023
Yale University
2010-2017
Michigan State University
2008-2014
Air Force Medical University
2013
Guilin University of Technology
2011
Zhejiang University
2009
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2009
Our knowledge of disease genes in neurological disorders is incomplete. With the aim closing this gap, we performed whole-exome sequencing on 143 multiplex consanguineous families whom known had been excluded by autozygosity mapping and candidate gene analysis. This prescreening step led to identification 69 recessive not previously associated with disease, which 33 are here described (SPDL1, TUBA3E, INO80, NID1, TSEN15, DMBX1, CLHC1, C12orf4, WDR93, ST7, MATN4, SEC24D, PCDHB4, PTPN23, TAF6,...
BST2/tetherin, an antiviral restriction factor, inhibits the release of enveloped viruses from cell surface. Human immunodeficiency virus-1 (HIV-1) antagonizes BST2 through viral protein u (Vpu), which downregulates We report crystal structure a complex containing Vpu and cytoplasmic domains core clathrin adaptor 1 (AP1). This, together with our biochemical functional validations, reveals how hijacks AP1-dependent membrane trafficking pathways to mistraffick BST2. mimics canonical acidic...
Tetherin/BST2 is a type-II membrane protein that inhibits the release of range enveloped viruses, including HIV-1. Here we report three crystal structures human tetherin, full-length ectodomain, triple cysteine mutant and an ectodomain truncation. These show tetherin forms continuous alpha helix encompassing almost entire ectodomain. Tetherin helices dimerize into parallel coiled coils via interactions throughout C-terminal portion A comparison multiple dimer reveals inherent constrained...
Escherichia coli glycogen synthase (EcGS, EC 2.4.1.21) is a retaining glycosyltransferase (GT) that transfers glucose from adenosine diphosphate to glucan chain acceptor with retention of configuration at the anomeric carbon. EcGS belongs GT-B structural superfamily. Here we report several x-ray structures together shed considerable light on structure and function these enzymes. The wild-type enzyme bound ADP revealed 15.2 degrees overall domain-domain closure provided for first time...
Protein reengineering of cellular retinoic acid binding protein II (CRABPII) has yielded a genetically addressable system, capable profluorophoric chromophore that results in fluorescent protein/chromophore complexes. These complexes exhibit far-red emission, with high quantum efficiencies and brightness also excellent pH stability spanning the range 2–11. In course this study, it became evident single mutations L121E R59W were most effective improving characteristics CRABPII mutants as well...
Adaptor proteins (APs) are critical components of the cellular membrane transport machinery. They mediate cargo selection during endocytosis and intracellular vesicular trafficking. Five AP complexes have been characterized (AP1-5), together their roles extend to diverse processes including homeostasis membranous organelles, protein turnover, immune responses. The evolution Human Immunodeficiency Virus type 1 (HIV-1) has exploited these complexes, enabling evasion assembly maximally...
ABSTRACT HIV-1 Nef binds to the cytoplasmic region of HLA-A and HLA-B downregulates these molecules from surface virus-infected cells, thus evading immune detection by CD8 + T cells. Polymorphic residues within HLA may affect Nef’s downregulation activity. However, effects polymorphisms on recognition primary isolates remain elusive, as do specific regions responsible for versus HLA-B. Here, we examined 46 clones isolated chronically subtype B-infected subjects their ability downregulate...
Cellular membrane proteins in the SERINC family, especially SERINC5, inhibit infectivity of retroviral virions. This inhibition is counteracted by proteins, specifically, HIV-1 Nef, MLV glycoGag, and EIAV S2. One consequence such a host-pathogen “arms race” compensatory change host antiviral protein as it evolves to escape effects viral antagonists. often reflected genetic signature, positive selection, which conspicuously missing SERINC5 . Here we show that despite this lack evidence,...
Although 70% (or 2/3) partial hepatectomy (PH) is the most studied model for liver regeneration, hepatic protein expression profile associated with lower volume resection (such as 50% PH) has not yet been reported. Therefore, aim of this study was to determine global regenerating mouse following PH by differential proteomics, and thereby gaining some insights into regeneration mechanism(s) under milder but clinically more relevant condition.Proteins from sham-operated livers 24 h after were...
HIV ‐1 Vpu modulates cellular transmembrane proteins to optimize viral replication and provide immune‐evasion, triggering ubiquitin‐mediated degradation of some targets but also modulating endosomal trafficking deplete them from the plasma membrane. Interactions between heterotetrameric clathrin adaptor protein (AP) complexes AP‐1 AP ‐2 have been described, yet molecular basis functional roles such interactions are incompletely defined. To investigate signals encoded by Vpu, we fused...
The structural integrity of cellular retinoic acid-binding protein II (CRABPII) has been investigated using the crystal structures CRABPII mutants. overall fold was well maintained by these mutants, each which carried multiple different mutations. A water-mediated network is found to be present across large binding cavity, extending from Arg111 deep inside cavity alpha2 helix at its entrance. This chain interactions acts as a ;pillar' that maintains protein. disruption water upon loss leads...
Cellular Retinoic Acid Binding Protein II (CRABPII) has been reengineered to specifically bind and react with all-trans-retinal form a protonated Schiff base. Each step of this process dissected four residues (Lys132, Tyr134, Arg111, Glu121) within the CRABPII binding site have identified as crucial for imine formation and/or protonation. The precise role each residue examined through directed mutagenesis crystallographic studies. crystal structure R132K:L121E-CRABPII (PDB-3I17) double...
To investigate the mechanism of Cr3+ ion translocation across cell membranes, interaction between and mimic biomembrane solid supported lipid bilayer (s-BLM) has been investigated quantitatively. The was prepared according to self-assemble phosphatidylcholiner(PC)/cholesterol(Ch) membrane on Pt electrode. Using ferricyanide anions species as a redox probe, cyclic voltammetric (CV), Alternating Current (A.C.) impedance spectroscopy (EIS), scanning electrochemical microscopy (SECM) approach...
The HIV-1 protein Nef suppresses multiple immune surveillance mechanisms to promote viral pathogenesis 1 . Individuals infected with encoding defective nef genes do not develop AIDS for decades 2,3 A key target of is the cellular receptor CD4. Although essential entry into host cells, CD4 problematic virus later in its replication cycle: disrupts processing glycoprotein, Env, inhibiting infectivity 4 ; it interferes release new virions 5,6 and causes vulnerability superinfection, causing...