A5005405921- Developmental Biology and Gene Regulation
- Congenital heart defects research
- MicroRNA in disease regulation
- interferon and immune responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Hearing, Cochlea, Tinnitus, Genetics
- Fuel Cells and Related Materials
- Genomics and Chromatin Dynamics
- Extracellular vesicles in disease
- Childhood Cancer Survivors' Quality of Life
- Molecular Biology Techniques and Applications
- Autism Spectrum Disorder Research
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Thermal properties of materials
- Blood Pressure and Hypertension Studies
- Cancer-related molecular mechanisms research
- Phosphodiesterase function and regulation
- Congenital Heart Disease Studies
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Animal Vocal Communication and Behavior
- Heart Failure Treatment and Management
- Zebrafish Biomedical Research Applications
University of California, San Francisco
2023
University of Colorado Anschutz Medical Campus
2019-2022
University of Colorado Boulder
2016-2017
University of Colorado Denver
2017
University of Colorado System
2016
Boston Children's Hospital
2014
Identifying therapeutics to delay, and potentially reverse, age-related cognitive decline is critical in light of the increased incidence dementia-related disorders forecasted growing older population1. Here we show that platelet factors transfer benefits young blood ageing brain. Systemic exposure aged male mice a fraction plasma from containing platelets decreased neuroinflammation hippocampus at transcriptional cellular level ameliorated hippocampal-dependent impairments. Circulating...
Vertebrate jaw development is coordinated by highly conserved ligand-receptor systems such as the peptide ligand Endothelin 1 (Edn1) and receptor type A (Ednra), which are required for patterning of lower structures. The Edn1/Ednra signaling pathway establishes identity progenitor cells regulating expression numerous genes, but intracellular mechanisms linking activation to gene regulation remain poorly understood. As a first step towards elucidating this mechanism, we examined function...
Stimulation of the renin-angiotensin-aldosterone system (RAAS) and β-adrenergic receptors plays an important role in adult heart failure (HF). Despite demonstrated benefits RAAS inhibition receptor blockade HF patients, no substantial improvement survival rate has been observed children with HF. This suggests that underlying disease mechanism is uniquely regulated pediatric Here, we show treatment human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) neonatal rat ventricular...
During craniofacial development, different populations of cartilage- and bone-forming cells develop in precise locations the head. Most these are derived from pluripotent cranial neural crest differentiate with distinct developmental timing cellular morphologies. The mechanisms that divide into discrete not fully understood. Here, we use single-cell RNA sequencing to transcriptomically define cells. We discovered gene family encoding Alx transcription factors is enriched frontonasal...
Human faces are variable; we look different from one another. Craniofacial disorders further increase facial variation. To understand craniofacial variation and how it can be buffered, analyzed the zebrafish mef2ca mutant. When this transcription factor encoding gene is mutated, develop dramatically variable phenotypes. Years of selective breeding for low high penetrance mutant phenotypes produced strains that either resilient or sensitive to mutation. Here, compared expression between these...
Deleterious genetic mutations allow developmental biologists to understand how genes control development. However, not all loss of function mutants develop phenotypic changes. Many deleterious only produce a phenotype in subset mutant individuals, phenomenon known as incomplete penetrance. Incomplete penetrance can confound analyses gene and our understanding this widespread remains inadequate. To better what controls penetrance, we capitalized on the zebrafish mef2ca which produces...
Mutations in the gene encoding zinc-finger transcription factor Ikaros (
The Notch pathway is a cell-cell communication system which critical for many developmental processes, including craniofacial development. receptor activation induces expression of several well-known canonical targets those encoded by the hes and her genes in mammals zebrafish, respectively. function these genes, individually combination, during development not well understood. Here, we used zebrafish genetics to investigate her9 her6 gene We found that required osteoblasts efficiently...
Abstract Vertebrate jaw development is coordinated by highly conserved ligand-receptor systems such as the peptide ligand Endothelin 1 (Edn1) and receptor type A (Ednra), which are required for patterning of lower structures. The Edn1/Ednra signaling pathway establishes identity progenitor cells regulating expression numerous genes, but intracellular mechanisms linking activation to gene regulation remain poorly understood. As a first step towards elucidating this mechanism, we examined...
ABSTRACT Human faces are variable; we look different from one another. Craniofacial disorders further increase this variability. Here used the zebrafish mef2ca mutant, which produces variable phenotypes, to understand craniofacial variation. Comparing alleles demonstrated that severity, measured by penetrance and expressivity, correlates with Years of selective breeding for low high produced strains either resilient, or sensitive, mutation. these demonstrates severity Gene expression studies...