A5044150113- T-cell and Retrovirus Studies
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Fungal and yeast genetics research
- NF-κB Signaling Pathways
- Galectins and Cancer Biology
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Immune Cell Function and Interaction
- Protein Degradation and Inhibitors
- RNA regulation and disease
- Virus-based gene therapy research
- Multiple Myeloma Research and Treatments
- Bacterial Genetics and Biotechnology
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- Heat shock proteins research
- Animal Virus Infections Studies
- Rabies epidemiology and control
- Viral-associated cancers and disorders
- Enzyme Structure and Function
- Hydrogen's biological and therapeutic effects
- Diet and metabolism studies
- Viral Infections and Outbreaks Research
Oita University
2015-2024
Universiti of Malaysia Sabah
2024
Oita University of Nursing and Health Sciences
2020
National Institutes of Health
2000-2008
National Institute of Allergy and Infectious Diseases
2002-2008
University of Yamanashi
1998-2006
University of Yamanashi Hospital
2000
National Institute of Genetics
1997
Kyoto Prefectural University
1989-1990
The gene coding for Bacillus cereus ATCC7064 (mesophile) oligo‐1,6‐glucosidase was cloned within a 2.8‐kb Sal I— Eco RI fragment of DNA, using the plasmid pUC19 as vector and Escherichia coli C600 host. E. bearing hybrid pBCE4 accumulated in cytoplasm. enzyme coincided absolutely with B. its M r (65000), electrophoretic behavior on polyacrylamide gel or without sodium dodecyl sulfate, isoelectric point (4.5), temperature dependence stability activity, antigenic determinants. nucleotide...
Human T-cell leukemia virus type 1 (HTLV-1) encodes a 40-kDa Tax phosphoprotein. is transcriptional activator which modulates expression of the viral long terminal repeat and transcription many cellular genes. Because critical HTLV-1 factor mediates transformation T cells during genesis adult leukemia, it important to understand processes can activate or inactivate function. Here, we report that ubiquitination posttranscriptional mechanism regulates We show does not target for degradation by...
Abstract Both natural viral infections and therapeutic interventions using vectors pose significant risks of malignant transformation. Monitoring for clonal expansion infected cells is important detecting cancer. Here we developed a novel method tracking clonality via the detection transgene integration sites. RAISING (Rapid Amplification Integration Sites without Interference by Genomic DNA contamination) sensitive, inexpensive alternative to established methods. Its compatibility with...
Abstract We evaluated the clinical benefits of novel predictive markers for distant recurrence with colorectal cancer using lectin microarray analysis cell surface glycan modifications. Glycoproteins were extracted from formalin‐fixed, paraffin‐embedded tumor specimens and normal epithelium 53 consecutive curatively resected stage I–III cases then subjected to obtain lectin–glycan interaction ( LGI ) values. In addition, clinicopathological factors associated identified. values that...
Abstract The activation of nuclear factor kappa B (NF-κB) signaling has a central role in the development adult T-cell leukemia/lymphoma (ATL) and many other cancers. However, mechanism NF-κB pathways remains poorly understood. Recently, we reported that N-myc downstream-regulated gene 2 (NDRG2) is negative regulator phosphoinositide 3-kinase (PI3K)/AKT pathway by promoting active dephosphorylated form PTEN at its C-terminus via recruitment PP2A. Additionally, down-regulation NDRG2...
Human T-cell leukemia virus type 1 (HTLV-1)-associated diseases are poorly treatable, and HTLV-1 vaccines not available. High proviral load is one major risk factor for disease development. encodes Tax oncoprotein, which activates transcription from viral long terminal repeats (LTR) various types of cellular promoters. Counteracting function might have prophylactic therapeutic benefits. In this work, we report on the suppression activation LTR by SIRT1 deacetylase. The transcriptional...
Diffuse Large B-cell Lymphoma (DLBCL), with its intrinsic genetic and epigenetic heterogeneity, exhibits significantly variable clinical outcomes among patients treated the current standard regimen. Disulfidptosis, a novel form of regulatory cell death triggered by disulfide stress, is characterized collapse cytoskeleton proteins F-actin due to intracellular accumulation disulfides. We investigated expression variations disulfidptosis-related genes (DRGs) in DLBCL using two publicly...
In the peripheral blood leukocytes (PBLs) from carriers of human T-lymphotropic virus type-1 (HTLV-1) or patients with adult T-cell leukemia (ATL), nuclear factor kappaB (NF-κB)-mediated antiapoptotic signals are constitutively activated primarily by HTLV-1-encoded oncoprotein Tax. Tax interacts I κB kinase regulatory subunit NEMO (NF-κB essential modulator) to activate NF-κB, and this interaction is maintained in part a molecular chaperone, heat-shock protein 90 (HSP90), its co-chaperone...
We have previously reported that Salvador homologue 1 (SAV1), a component of the Hippo pathway, is significantly down-regulated in high-grade clear cell renal carcinoma (ccRCC) due to 14q copy number loss, and this down-regulation contributes proliferation survival tubular epithelial cells through activation Yes-associated protein (YAP1), downstream target pathway. However, impact SAV1 loss on kidney vivo remained be determined. To address issue, we generated kidney-specific Sav1-knockout...
Adult T-cell leukemia/lymphoma (ATL) is a highly chemoresistant malignancy of peripheral T lymphocytes caused by human leukemia virus type 1 infection, for which there an urgent need more effective therapeutic options. The molecular chaperone heat shock protein 90 (HSP90) plays crucial role in nuclear factor-κB (NF-κB)-mediated antiapoptosis ATL cells, and HSP90 inhibitors are new candidate therapeutics ATL. Accordingly, we investigated the anti-ATL effects novel oral inhibitor, TAS-116...
It is widely accepted that aberrant activation of the Wnt signaling pathway responsible for development precursor lesions colorectal cancer (CRC). However, molecular mechanisms involved in process progression from these to invasive CRC are not fully understood. Recently, we reported constitutive MAPK accompanied by downregulation dual‐specificity phosphatase 4 (DUSP4), a phosphatase, contributes pancreas. In this study, found DUSP4 was related ERKs cells. Restoration resulted inactivation...
N-myc downstream-regulated gene 2 (NDRG2), which is a tumour suppressor, frequently lost in many types of tumours, including adult T-cell leukaemia/lymphoma (ATL). The downregulation NDRG2 expression involved progression through the aberrant phosphorylation several important signalling molecules. We observed that induced translocation protein arginine methyltransferase 5 (PRMT5) from nucleus to cytoplasm via increased PRMT5 at Serine 335. In NDRG2low ATL, cytoplasmic enhanced HSP90A...
ABSTRACT The human T-cell leukemia virus type 1 (HTLV-1) Tax oncoprotein can repress the transcriptional activity of tumor suppressor protein p53. However, it remains controversial whether requires NF-κB factors/activity and/or p300/CBP in order to inactivate p53 function. To address this issue, we have investigated Tax's effect on p53's activation IκB-kinase-deficient mouse embryonic fibroblasts (MEFs); some which are entirely silent for Tax-induced activity. We found that, IKKα −/− , IKKβ...
Gastric cancer ( GC ) is characterized by amplifications of receptor tyrosine kinases RTK and KRAS , therefore, targeting the / downstream pathways could help to broaden applicability molecular targeted therapy for . We assembled a panel 48 cell lines screened predictors responsiveness inhibition RAF MEK ERK pathway, one pathways. found that cells with MET amplification or mutation, but not amplification, tended be sensitive inhibition. However, several without alterations also showed high...
In patients with esophageal squamous cell carcinoma (ESCC), the status of metastasis to lymph nodes is strongly associated prognosis. Consequently, development a biomarker detect presence would be clinically valuable. this study, we found that overexpression cannabinoid receptor 1 (CB1R) was applicable as marker for prediction in ESCC. CB1R detected immunohistochemically 54 88 cases (61.4%). The intensity expression uniform both intraepithelial and invasive regions each case, significantly...
Background Diarrhea is a major cause of morbidity and mortality among Bhutanese children. The etiology diarrhea not well known due to the challenges conducting routine surveillance with Bhutan's modest research facilities. Establishing an crucial toward generating evidence that will contribute policy discussions on diarrheal disease control program. Our previous study, during 2010–2012, revealed norovirus (NoV) important children, GII.21 was genotype circulating at time. In other countries,...