A5013723882- Reproductive System and Pregnancy
- Estrogen and related hormone effects
- Ion channel regulation and function
- Endometriosis Research and Treatment
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Ovarian function and disorders
- Reproductive Biology and Fertility
- Aortic Disease and Treatment Approaches
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Multiple Sclerosis Research Studies
- Nicotinic Acetylcholine Receptors Study
- Aortic aneurysm repair treatments
- Hypothalamic control of reproductive hormones
- Neurobiology and Insect Physiology Research
- Endometrial and Cervical Cancer Treatments
- French Historical and Cultural Studies
- Infectious Aortic and Vascular Conditions
- Erythropoietin and Anemia Treatment
- Dialysis and Renal Disease Management
- Insect Resistance and Genetics
- Hormonal and reproductive studies
- Cardiac Valve Diseases and Treatments
- Platelet Disorders and Treatments
Indiana University
2024-2025
University School
2025
Indiana University – Purdue University Indianapolis
2025
The University of Texas Health Science Center at San Antonio
2014-2023
American Airlines (United States)
2023
State Board of Education
2023
The University of Texas Health Science Center at Houston
2012-2021
Integrative Health Technologies (United States)
2020
Royal Free London NHS Foundation Trust
2016-2019
University of Minnesota
2005-2018
Complementary DNAs were isolated and used to deduce the primary structures of alpha 1 2 subunits dihydropyridine-sensitive, voltage-dependent calcium channel from rabbit skeletal muscle. The subunit, which contains putative binding sites for antagonists, is a hydrophobic protein with sequence that consistent multiple transmembrane domains shows structural homology other ion channels. In contrast, subunit hydrophilic without known sequences. Nucleic acid hybridization studies suggest mRNAs...
We present the cloning and characterization of two novel calcium-activated potassium channel β subunits, hKCNMB3 hKCNMB4, that are enriched in testis brain, respectively. compare contrast steady state kinetic properties these subunits with previously cloned mouse β1 (mKCNMB1) human β2 subunit (hKCNMB2). Once inactivation is removed, we find hKCNMB2 has similar to mKCNMB1. slows<i>Hslo1</i> gating shifts current-voltage relationship more negative potentials. hKCNMB4 have distinct effects on...
Basal body replication during estrogen-driven ciliogenesis in the rhesus monkey (Macaca mulatta) oviduct has been studied by stereomicroscopy, rotation photography, and serial section analysis. Two pathways for basal production are described: acentriolar formation (major pathway) where procentrioles generated from a spherical aggregate of fibers; centriolar formation, diplosomal centrioles. In both pathways, first step procentriole is arrangement fibrous granule precursor into an annulus. A...
Both estradiol and progesterone may act locally to modulate ovarian function in various species. This study examined the distribution of receptors (ER PR, respectively) within primate ovary throughout menstrual cycle. Ovaries were collected from rhesus or cynomolgus monkeys during early, mid-, late (n = 3-6/stage) follicular luteal phases The tissues processed for indirect immunocytochemical localization with specific monoclonal antibodies against ER (H222 D75) PR (JZB39). Specific staining,...
Estrogen action is dependent upon the presence of specific ligand-activated receptors in target tissues. The aim present experiments was to compare spatial and temporal pattern expression estrogen receptor β (ERβ) with that ERα full thickness endometrial samples (from superficial basal zone) obtained from both women rhesus macaques. Immunohistochemical localization antibodies revealed ERβ were expressed nuclei glands stroma. Consistent previous studies, declined stroma functionalis during...
The levels of estradiol and progesterone in the systemic plasma four domestic cats during pregnancy, pseudopregnancy polyestrus were determined by radioimmunoassay. During polyestrus, values fluctuated between peaks (59.5 ± 13.4 (SD) pg/ml; n = 13) troughs (8.1 3.8 n=12) with an interpeak period 15.8 days (n=9). After animals had been mated to either intact or vasectomized males, their declined sharply from peak remained low (6–12 pg/ml) pregnancy except for a slight rise just before...
In vitro studies have shown that keratinocyte growth factor (KGF, also known as FGF-7) is secreted by fibroblasts and mitogenic specifically for epithelial cells. Therefore, KGF may be an important paracrine mediator of cell proliferation in vivo. Because stromal cells are thought to influence glandular the primate endometrium, we investigated hormonal regulation cellular localization mRNA expression rhesus monkey uterus. Tissues were obtained both from naturally cycling monkeys follicular...
Despite extensive literature on vascular endothelial growth factor (VEGF) expression and regulation by steroid hormones, the lack of clear understanding mechanisms angiogenesis in endometrium is a major limitation for use antiangiogenic therapy targeting endometrial vessels. In current work, we used rhesus macaque as primate model decidualized mouse uterus murine to examine during breakdown regeneration. We found that blockade VEGF action with Trap, potent blocker, completely inhibited...
Abstract The hamster uterine luminal epithelium undergoes cyclic morphological changes, which include mitosis and hypertrophy prior to ovulation degenerative changes after ovulation. We have previously shown that the are symptoms of estradiol (E 2 ) withdrawal; they occur within 24 hours ovariectomy, can be prevented by E treatment, associated with a decline in content nuclear receptor (West et al., 1978). In this report, results an electron microscopic study cycling hormonally altered...
AKI after cardiac surgery remains strongly associated with mortality and lacks effective treatment or prevention. Preclinical studies suggest that cell-based interventions may influence functional recovery. We conducted a phase 2, randomized, double-blind, placebo-controlled trial in 27 centers across North America to determine the safety efficacy of allogeneic human mesenchymal stem cells (MSCs) reducing time recovery from surgery. randomized 156 adult subjects undergoing evidence early...
Post-traumatic stress disorder (PTSD) is characterized by exaggerated fear expression and impaired extinction. The underlying molecular cellular mechanisms of PTSD are largely unknown. current pharmacological non-pharmacological treatments for either ineffective or temporary with high relapse rates. Here we report that adiponectin-deficient mice exhibited normal contextual conditioning but displayed slower extinction learning. Infusions adiponectin into the dentate gyrus (DG) hippocampus in...
1 The large-conductance calcium-activated potassium (BK) channel plays an important role in controlling membrane potential and contractility of urinary bladder smooth muscle (UBSM). These channels are composed a pore-forming α-subunit accessory, muscle-specific, β1-subunit. 2 Our aim was to determine the functional β1-subunit BK contractions UBSM by using 'knock-out' (KO) mice. 3 β-galactosidase reporter (lacZ gene) targeted β1 locus, which provided opportunity examine expression UBSM. Based...
The relationship between vascular endothelial growth factor (VEGF) expression and the pattern of proliferation in rhesus macaque endometrium has not been studied. In this report, we used situ hybridization to evaluate VEGF, VEGF receptor type 1 2 mRNA during hormonally regulated menstrual cycles ovariectomized macaques. Proliferating cells were identified by a double immunocytochemistry procedure that detected Ki-67 antigen von Willebrand same cells. One d after progesterone withdrawal...