A5012343805- Neuroscience and Neuropharmacology Research
- Epilepsy research and treatment
- Ion channel regulation and function
- Electrochemical Analysis and Applications
- Neonatal and fetal brain pathology
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Connexins and lens biology
- Neuroscience and Neural Engineering
- Adenosine and Purinergic Signaling
- Stress Responses and Cortisol
- Traumatic Brain Injury and Neurovascular Disturbances
- Receptor Mechanisms and Signaling
- Electrochemical sensors and biosensors
- Memory and Neural Mechanisms
- Anesthesia and Neurotoxicity Research
- Neuroscience of respiration and sleep
- Amino Acid Enzymes and Metabolism
- bioluminescence and chemiluminescence research
- Nerve injury and regeneration
- Tryptophan and brain disorders
- GABA and Rice Research
- Advanced biosensing and bioanalysis techniques
- Synthesis and biological activity
- Chemical synthesis and pharmacological studies
Universidad de Guadalajara
2006-2018
Abstract: It is well documented that neurons exposed to high concentrations of excitatory amino acids, such as glutamate and aspartate, degenerate die. The clearance these acids from the synaptic cleft depends mainly on their transport by high‐affinity sodium‐dependent carriers. Using microdialysis in vivo HPLC analysis, we have studied effect administration inhibitors transporter ( l ‐ trans ‐pyrrolidine‐2,4‐dicarboxylate dihydrokainate) extracellular concentration endogenous rat striatum....
Abstract Study Objectives: To examine the pattern of extracellular adenosine in human brain during sleep deprivation, sleep, and normal wake. Design: Following recovery from implantation clinical depth electrodes, epilepsy patients remained awake for 40 continuous hours, followed by a episode. Setting: Neurology ward at UCLA Medical Center. Patients or Participants: Seven male undergoing electrode localization pharmacologically refractory seizures. Interventions: All subjects were implanted...
Introduction The fluid percussion method is widely used to induce brain injury in rodents. However, this approach has several limitations, including variability the resulting damage, which attributed factors such as manual control of mass generate desired pressure. To address these issues, modifications original have been proposed. Methods In study, we present a novel device called Hydro-pneumatic Fluid Percussion Device, delivers directly lateral region injury. validate model, three groups...
Glutamate has been measured using different methods to determine its role under normal and pathological conditions. Although microdialysis coupled with HPLC is the preferred method study glutamate, this technique exhibits poor temporal resolution time consuming. The concentration of glutamate in dialysis samples can be via oxidase Amplex Red method. A new device designed constructed rapidly deposit onto a polycarbonate plate at Cartesian coordinates (every five seconds). were added an...
In epilepsy, seizures are generated by abnormal synchronous activity in neurons. the rat hippocampus (HIP), epileptiform has been found to be associated with gap junctions (GJs). GJs formed combination of two hemichannels, each composed six connexins. At low doses, convulsive drug 4-aminopyridine (4-AP) produces without affecting glutamate levels; therefore, could participate its effect. Based on this argument, study, expression Cx 32, 36 and 43 protein mRNA HIP rats treated 4-AP was...
Epilepsy is a neurological disorder produced by an imbalance between excitatory and inhibitory neurotransmission, in which transporters of both glutamate GABA have been implicated. Hence, at different times after local administration the convulsive drug 4-aminopyridine (4-AP) we analyzed expression EAAT-3 GAT-1 transporter proteins cells CA1 dentate gyrus.Dual immunofluorescence was used to detect co-localization neuronal marker. In parallel, EEG recordings were performed behavior rated...
Status epilepticus (SE) can result in an overproduction of hydrogen peroxide (H2O2), which contributes to oxidative stress and brain injury during different phases epileptogenesis seizures. In this study, we measured the extracellular H2O2 concentration rat hippocampus a temporal lobe epilepsy model. A new fluorescent technique for measuring vivo simultaneously with electroencephalography recording was tested. The method consists mixing microdialysate enzymatic reactor produce compound....
One of the most important manifestations perinatal asphyxia is occurrence seizures, which are treated with antiepileptic drugs, such as carbamazepine. These early combined pharmacological treatments, may influence development dopaminergic neurotransmission in frontal cortex. This study aimed to determine extracellular levels dopamine and its main metabolite DOPAC 30-day-old rats that had been asphyxiated for 45 min a low (8%) oxygen chamber at age daily doses Quantifications were performed...
Abstract Glutamate measurement in microdialysis samples has primarily been determined using HPLC methods, and several attempts have made to establish a relationship between this neurotransmitter EEG activity during altered brain function, such as epilepsy. However, classic methods lack high temporal resolution. In study, new alternative is proposed improve the time resolution thus obtain better understanding of dynamics Glu its with epileptiform activity. A setup was designed measure online...