A5101701749- Parasitic Diseases Research and Treatment
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Biosensors and Analytical Detection
- Parasite Biology and Host Interactions
- Trypanosoma species research and implications
- Herpesvirus Infections and Treatments
- Advanced biosensing and bioanalysis techniques
- Poxvirus research and outbreaks
- SARS-CoV-2 detection and testing
- Genetics, Aging, and Longevity in Model Organisms
- Nematode management and characterization studies
- Big Data Technologies and Applications
- Legionella and Acanthamoeba research
- Bacillus and Francisella bacterial research
- Insects and Parasite Interactions
- Studies on Chitinases and Chitosanases
- Seedling growth and survival studies
- Winter Sports Injuries and Performance
- Aquatic life and conservation
- Underground infrastructure and sustainability
- RNA and protein synthesis mechanisms
- Botanical Research and Applications
- Yeasts and Rust Fungi Studies
- Lysosomal Storage Disorders Research
New England Biolabs (United States)
2012-2024
Northwest A&F University
2023
National Institute for Communicable Disease Control and Prevention
2023
New England Biolabs (China)
2021
Shenyang University of Technology
2021
Northeast Agricultural University
2020
Tufts University
2014-2019
South China University of Technology
2010
Sichuan University
2008
Fred Hutch Cancer Center
2000
Accurate detection of filarial parasites in humans is essential for the implementation and evaluation mass drug administration programs to control onchocerciasis lymphatic filariasis. Determining infection levels vector populations also important assessing transmission, deciding when treatments may be terminated monitoring recrudescence. Immunological methods detect are available, however, cross-reactivity issues have been reported. Nucleic acid-based molecular assays offer high specificity...
Abstract Glycolytic interconversion of phosphoglycerate isomers is catalysed in numerous pathogenic microorganisms by a cofactor-independent mutase (iPGM) structurally distinct from the mammalian cofactor-dependent (dPGM) isozyme. The iPGM active site dynamically assembles through substrate-triggered movement phosphatase and transferase domains creating solvent inaccessible cavity. Here we identify alternate ligand binding regions using nematode to select enrich lariat-like ligands an...
Wolbachia, an alpha-proteobacterium closely related to Rickettsia, is a maternally transmitted, intracellular symbiont of arthropods and nematodes. Aedes albopictus mosquitoes are naturally infected with Wolbachia strains wAlbA wAlbB. Cell line Aa23 established from Ae. embryos retains only wAlbB key model study host-endosymbiont interactions. We have assembled the complete circular genome cell using long-read PacBio sequencing at 500× median coverage. The chromosome 1.48 megabases in size,...
Effective management of the COVID-19 pandemic requires widespread and frequent testing population for SARS-CoV-2 infection. Saliva has emerged as an attractive alternative to nasopharyngeal samples surveillance it does not require specialized personnel or materials its collection can be easily provided by patient. We have developed a simple, fast, sensitive saliva-based workflow that minimal sample treatment equipment. After inactivation, RNA is quickly released stabilized in optimized...
Conventional diagnosis of filarial infections is based on morphological identification microfilariae using light microscopy and requires considerable expertise, time-consuming, can be subjective. Loop-mediated isothermal amplification (LAMP) has advantages over or PCR because its operational simplicity, rapidity versatility readout options. LAMP assays represent a major step forward in improved diagnostic tools suitable for low resource settings field applicability. The study goal was to...
The human filarial parasite Brugia malayi harbors an endosymbiotic bacterium Wolbachia (wBm) that is required for survival. Consequently, targeting wBm a promising approach anti-filarial drug development. Type IV secretion system (T4SS) plays important role in bacteria-host interactions and under stringent regulation by transcription factors. In wBm, most T4SS genes are contained two operons. We show the active since essential assembly factor virB8-1, transcribed adult worms larval stages,...
The use of antibiotics targeting the obligate bacterial endosymbiont Wolbachia filarial parasites has been validated as an approach for controlling infection in animals and humans. Availability genomic sequences (wBm) present human parasite Brugia malayi enabled genome-wide searching new potential drug targets. In study, we investigated cell division machinery wBm determined that it possesses essential gene ftsZ which was expressed all developmental stages B. examined. FtsZ is a GTPase...
Parasitic nematodes are responsible for devastating illnesses that plague many of the world's poorest populations indigenous to tropical areas developing nations. Among these diseases is lymphatic filariasis, a major cause permanent and long-term disability. Proteins essential do not have mammalian counterparts represent targets therapeutic inhibitor discovery. One promising target trehalose-6-phosphate phosphatase (T6PP) from Brugia malayi. In model nematode Caenorhabditis elegans, T6PP...
Accurate, simple and affordable diagnostics are needed to detect Onchocerca volvulus infection in humans. A newly developed colorimetric loop-mediated isothermal amplification (LAMP) assay was compared PCR skin snip analysis for diagnosis of onchocerciasis. The robustness simplicity the indicates that it may be a useful field tool surveillance endemic countries.
The intracellular endosymbiotic proteobacteria Wolbachia have evolved across the phyla nematoda and arthropoda. In phylogeny, supergroup F is only clade known so far with members from both arthropod filarial nematode hosts therefore can provide unique insights into their evolution biology. this study, 4 new genomes been assembled using a metagenomic assembly binning approach, wMoz wMpe human parasites Mansonella ozzardi perstans, wOcae wMoviF blue mason bee Osmia caerulescens sheep ked...
The filarial parasites Mansonella ozzardi and perstans , causative agents of mansonellosis, infect hundreds millions people worldwide, yet remain among the most understudied human pathogens. M. is highly prevalent in Latin American countries Caribbean Islands, while predominantly found sub-Saharan Africa as well a few areas South America. In addition to differences their geographical distribution, two are transmitted by different insect vectors, exhibit responses commonly used anthelminthic...
Mpox is a neglected zoonotic disease endemic in West and Central Africa. The outbreak with more than 90,000 cases worldwide since 2022 generated great concern about future outbreaks highlighted the need for simple rapid diagnostic test. virus, MPV, member of Orthopoxvirus (OPV) genus that also contains other pathogenic viruses including variola vaccinia camelpox cowpox virus. Phylogenomic analysis 200 OPV genomes identified 10 distinct phylogroups New World OPVs placed on very long branch...
Myristoylation is a lipid modification involving the addition of 14-carbon unsaturated fatty acid, myristic to N-terminal glycine subset proteins, that promotes their binding cell membranes for varied biological functions. The process catalyzed by myristoyl-CoA:protein N-myristoyltransferase (NMT), an enzyme which has been validated as drug target in human cancers, and infectious diseases caused fungi, viruses protozoan parasites. We purified Caenorhabditis elegans Brugia malayi NMTs active...
Wolbachia is an unculturable, intracellular bacterium that persists within extremely broad range of arthropod and parasitic nematode hosts, where it transmitted maternally to offspring via vertical transmission. In the filarial Brugia malayi, a causative agent human lymphatic filariasis, endosymbiont, its presence essential for proper development, survival, pathogenesis. While elucidation Wolbachia:nematode interactions promote bacterium's persistence great importance, research has been...
Considering the importance of energy in our lives and its impact on other critical infrastructures, this paper starts from whole life cycle big data divides security privacy risk factors into five stages: collection, transmission, storage, use, destruction. Integrating consideration cloud environment, fully analyzes each stage establishes a assessment index system for data. According to different degrees impact, AHP method is used give indexes weights, genetic algorithm optimize initial...
To investigate the influence of three single nucleotide polymorphisms (SNPs) in exon 12 (C1236T), 21 (G2677T/A) and 26 (C3435T) MDR1 gene on absorption valacyclovir after a oral administration Chinese Han ethnic population.
Abstract Background During infection by intracellular pathogens, a highly complex interplay occurs between the infected cell trying to degrade invader and pathogen which actively manipulates host enable survival proliferation. Many pathogens pose important threats human health major efforts have been undertaken better understand host-pathogen interactions that eventually determine outcome of infection. Over last decades, unicellular eukaryote Dictyostelium discoideum has become an...
Filarial diseases, including lymphatic filariasis and onchocerciasis, are considered among the most devastating of all tropical affecting over 86 million people worldwide. To control more rapidly eliminate onchocerciasis requires treatments that target adult stage parasite. Drug discovery efforts challenged by lack preclinical animal models using human-pathogenic filariae, requiring use surrogate parasites for Onchocerca volvulus both ex vivo in evaluation. Herein, we describe a platform...
Abstract The filarial parasites Mansonella ozzardi and perstans , causative agents of mansonellosis, infect hundreds millions people worldwide, yet remain among the most understudied human pathogens. M. is highly prevalent in Latin American countries Caribbean Islands, while predominantly found sub-Saharan Africa as well a few areas South America. In addition to differences their geographical distribution, two are transmitted by different insect vectors, exhibit responses commonly used...