A5085822351- Wheat and Barley Genetics and Pathology
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Genetics and Plant Breeding
- Insect-Plant Interactions and Control
- Bioenergy crop production and management
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Genetic and Environmental Crop Studies
- Agricultural pest management studies
- Neurobiology and Insect Physiology Research
- Plant pathogens and resistance mechanisms
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Seed and Plant Biochemistry
- Food composition and properties
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Genomics, phytochemicals, and oxidative stress
- RNA and protein synthesis mechanisms
Edward T. Schafer Agricultural Research Center
2022-2025
North Dakota State University
2022-2024
Noble Research Institute
2019-2024
Agricultural Research Service
2022-2023
Oklahoma State University
2022
United States Department of Agriculture
2022
University of West Florida
2019
University of Florida
2019
University of California, Davis
2013-2016
Plant (United States)
2015
Toll/interleukin receptor (TIR) domain-containing proteins encoded in the Arabidopsis (Arabidopsis thaliana) genome include TIR-nucleotide binding site (TN) and TIR-unknown site/domain (TX) families. We investigated function of these proteins. Transient overexpression five TX TN genes tobacco (Nicotiana benthamiana) induced chlorosis. This chlorosis was dependent on ENHANCED DISEASE RESISTANCE1, a dependency conserved both Arabidopsis. Stable transgenic lines produced variety phenotypes...
Hexaploid oat (Avena sativa L.) is a commercially important cereal crop due to its soluble dietary fiber β-glucan, hemicellulose known prevent cardio-vascular diseases. To maximize health benefits associated with the consumption of oat-based food products, breeding efforts have aimed at increasing β-glucan content in groats. However, progress has been limited. accelerate efforts, we leveraged existing datasets (1,230 lines from South Dakota State University program grown multiple...
Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), Argonaute proteins (AGOs). However, the transcriptional regulation of these critical components largely unknown. In petunia (Petunia hybrida), an ethylene-responsive element binding factor, PhERF2, induced by Tobacco rattle virus (TRV) infection. Inclusion a PhERF2 fragment TRV construct containing reporter...
From a single transgenic line harboring five Tnt1 transposon insertions, we generated near-saturated insertion population in Medicago truncatula. Using thermal asymmetric interlaced-polymerase chain reaction followed by sequencing, recovered 388 888 flanking sequence tags (FSTs) from 21 741 lines this population. FST recovery 14 using the whole-genome sequencing (WGS) and/or Tnt1-capture approaches suggests an average of 80 insertions per line, which is more than previous estimation 25...
Legumes are of great interest for sustainable agricultural production as they fix atmospheric nitrogen to improve the soil. Medicago truncatula is a well-established model legume, and extensive studies in fundamental molecular, physiological, developmental biology have been undertaken translate into trait improvements economically important legume crops worldwide. However, M. reference genome was generated accession Jemalong A17, which highly recalcitrant transformation. R108 more attractive...
Summary Plants being sessile integrate information from a variety of endogenous and external cues simultaneously to optimize growth development. This necessitates the signaling networks in plants be highly dynamic flexible. One such network involves heterotrimeric G‐proteins comprised Gα, Gβ, Gγ subunits, which influence many aspects growth, development, stress response pathways. In as Arabidopsis, relatively simple repertoire one canonical three extra‐large Gβ subunits exists. Because...
Global warming and emerging plant diseases challenge agricultural food/feed production. We identify mechanism(s) regulating both thermotolerance disease resistance. Using virus-induced gene silencing (VIGS)-based genetic screening, we a thioredoxin-like 1 (TRXL1) involved in nonhost resistance thermotolerance. TRXL1 is reduced, partly degraded via proteases proteasome, alters its chloroplast localization during heat stress. interacts with more than 400 proteins, including chaperonin CPN60A,...
The glassy-winged sharpshooter Homalodisca vitripennis (Hemiptera: Cicadellidae), is a xylem-feeding leafhopper and important vector of the bacterium Xylella fastidiosa; causal agent Pierce's disease grapevines. functional complexity transcriptome H. has not been elucidated thus far. It necessary blueprint for an understanding development designing efficient biorational control strategies including those based on RNA interference.Here we elucidate explore adult using high-throughput paired...
Abstract Septoria nodorum blotch (SNB), caused by Parastagonospora nodorum, is a disease of durum and common wheat initiated the recognition pathogen-produced necrotrophic effectors (NEs) specific genes. The gene Snn1 encodes wall-associated kinase that directly interacts with NE SnTox1 leading to development SNB. Here, sequence analysis from 114 accessions including diploid, tetraploid hexaploid species revealed some lines possess two copies (designated Snn1-B1 Snn1-B2 ) approximately 120...
SUMMARY Septoria nodorum blotch (SNB), caused by Parastagonospora nodorum, is a disease of durum and common wheat initiated the recognition pathogen‐produced necrotrophic effectors (NEs) specific genes. The gene Snn1 was previously cloned, it encodes wall‐associated kinase that directly interacts with NE SnTox1 leading to programmed cell death ultimately development SNB. Here, sequence analysis from 114 accessions including diploid, tetraploid, hexaploid species revealed some lines possess...
Many plant-encoded E3 ligases are known to be involved in plant defense. Here, we report a novel role of ligase SALT- AND DROUGHT-INDUCED RING FINGER1 (SDIR1) immunity. Even though SDIR1 is reasonably well-characterized, its biotic stress response not known. The silencing Nicotiana benthamiana reduced the multiplication virulent bacterial pathogen Pseudomonas syringae pv. tabaci. Arabidopsis sdir1 mutant resistant pathogens, whereas overexpression lines susceptible both host and nonhost...
is a chosen model for legumes towards deciphering fundamental legume biology, especially symbiotic nitrogen fixation. Current genomic resources
Genetic characterization of a major spot form net blotch susceptibility locus to using linkage mapping identify candidate gene and user-friendly markers in barley. Spot (SFNB), caused by the necrotrophic fungal pathogen Pyrenophora teres f. maculata (Ptm), is an economically important foliar diseases Although various resistance loci have been identified, breeding for SFNB-resistant varieties has hampered due complex virulence profile Ptm populations. One host may be effective against one...
Puccinia novopanici is an important biotrophic fungal pathogen that causes rust disease in switchgrass. Lack of genomic resources for P. has hampered the progress toward developing effective resistance against this pathogen. Therefore, we have sequenced whole genome and generated a framework to understand pathogenicity mechanisms identify effectors, repeat element invasion, evolution, comparative genomics among spp. future. Long- short-read sequences were from DNA by PacBio Illumina...
Necrotrophic fungi constitute the largest group of plant fungal pathogens that cause heavy crop losses worldwide. Phymatotrichopsis omnivora is a broad host, soil-borne necrotrophic pathogen infects over 2,000 dicotyledonous plants. The molecular basis such host range unknown. We conducted cell biology and transcriptomic studies in Medicago truncatula (susceptible), Brachypodium distachyon (resistant/nonhost), Arabidopsis thaliana (partially resistant) to understand P. virulence mechanisms....
Brachypodium distachyon is an annual C3 grass used as a monocot model system in functional genomics research. Insertional mutagenesis powerful tool for both forward and reverse genetics studies. In this study, we explored the possibility of using tobacco retrotransposon Tnt1 to create transposon-based insertion mutant population B. distachyon. We developed transgenic plants expressing (R0) subsequent regenerants (R1) observed that actively transposed during somatic embryogenesis, generating...
The glassy-winged sharpshooter (GWSS) Homalodisca vitripennis (Hemiptera: Cicadellidae), is a xylem-feeding leafhopper and an important vector of the bacterium Xylella fastidiosa; causal agent Pierce's disease grapevines. MicroRNAs are class small RNAs that play role in functional development various organisms including insects. In H. vitripennis, we identified microRNAs using high-throughput deep sequencing adults followed by computational manual annotation. A total 14 novel not found...
Epoxide hydrolase (EH) is an enzyme in the α/β-hydrolase fold superfamily that uses a water molecule to transform epoxide its corresponding diol. In insects, EHs metabolize among other things critical developmental hormones called juvenile (JHs). also play roles detoxification of toxic compounds are found insect's diet or environment. this study, full-length cDNA encoding hydrolase, Hovi-mEH1, was obtained from xylem-feeding insect Homalodisca vitripennis. H. vitripennis, commonly known as...