A5025250055- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Electrochemical sensors and biosensors
- Microbial Community Ecology and Physiology
- Mosquito-borne diseases and control
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Microbial Fuel Cells and Bioremediation
- Advanced battery technologies research
- Viral Infections and Vectors
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- HIV Research and Treatment
Max Planck Institute for Terrestrial Microbiology
2023-2024
Joint BioEnergy Institute
2018-2020
Lawrence Berkeley National Laboratory
2020
Technical University of Darmstadt
2020
University of Tübingen
2011
Electricity is paramount to the technical world and plays an increasingly important role as a future energy carrier. Yet, it not widely used directly power biological systems. Here, we designed new-to-nature electrobiological module, acid/aldehyde ATP cycle (AAA cycle), for direct conversion of electrical into ATP. The AAA contains minimum set enzymes does require membrane-based charge separation. Realizing propionate-based version cycle, demonstrate continuous, electricity-driven...
Dengue virus (DENV) is a member of the Flaviviridae and globally (re)emerging pathogen that causes serious human disease. There no specific antiviral or vaccine for dengue infection. Flavivirus capsid (C) structural protein responsible gathering viral RNA into nucleocapsid forms core mature particle. Flaviviral replication known to occur in cytoplasm yet large portion localizes nucleus during The reasons nuclear presences are not completely understood. Here, we expressed DENV C tandem...
Enzymatic oligonucleotide synthesis methods based on the template-independent polymerase terminal deoxynucleotidyl transferase (TdT) promise to enable de novo of long oligonucleotides under mild, aqueous conditions. Intermediates with a 3′ structure (hairpins) will inevitably arise during synthesis, but TdT has poor activity these structured substrates, limiting its usefulness for synthesis. Here, we described two parallel efforts improve hairpins: (1) optimization concentrations divalent...
Abstract The hydroxyacid glycolate is a highly abundant carbon source in the environment. Glycolate produced by unicellular photosynthetic organisms and excreted at petagram scales to environment, where it serves as growth substrate for heterotrophic bacteria. In microbial metabolism, first oxidized glyoxylate enzyme oxidase. recently described β-hydroxyaspartate cycle (BHAC) subsequently mediates carbon-neutral assimilation of into central metabolism ubiquitous Alpha- Gammaproteobacteria....
Abstract In vitro metabolic systems allow the reconstitution of natural and new-to-nature pathways outside their cellular context are increasing interest in bottom-up synthetic biology, cell-free manufacturing engineering. Yet, prototyping such networks is very often restricted by time- cost-intensive analytical methods. To overcome these limitations, we sought to develop an transcription (IVT)-based biosensing workflow that offers fast results at low-cost, minimal volumes high-throughput....
The hydroxyacid glycolate is a highly abundant carbon source in the environment. Glycolate produced by unicellular photosynthetic organisms and excreted at petagram scales to environment, where it serves as growth substrate for heterotrophic bacteria. In microbial metabolism, first oxidized glyoxylate enzyme oxidase. recently described β-hydroxyaspartate cycle (BHAC) subsequently mediates carbon-neutral assimilation of into central metabolism ubiquitous Alpha- Gammaproteobacteria. Although...
Abstract In vitro metabolic systems allow the reconstitution of natural and new-to-nature pathways outside their cellular context are increasing interest in bottom-up synthetic biology, cell-free manufacturing, engineering. Yet, analysis activity such networks is very often restricted by time- cost-intensive methods. To overcome these limitations, we sought to develop an transcription (IVT)-based biosensing workflow that compatible with complex conditions metabolism, as...
Promiscuous enzymes often serve as the starting point for evolution of novel functions. Yet, extent to which promiscuity an individual enzyme can be harnessed several times independently different purposes during is poorly reported. Here, we present a case study illustrating how NAD(P)