A5052034572- Lipid Membrane Structure and Behavior
- Ion channel regulation and function
- RNA Interference and Gene Delivery
- Protein Structure and Dynamics
- Calcium signaling and nucleotide metabolism
- Ion Channels and Receptors
- Supramolecular Self-Assembly in Materials
- Drug Transport and Resistance Mechanisms
- Microbial Inactivation Methods
- Photoreceptor and optogenetics research
- Spectroscopy and Quantum Chemical Studies
- Biochemical effects in animals
- Microfluidic and Bio-sensing Technologies
- Erythrocyte Function and Pathophysiology
- Ion Transport and Channel Regulation
- Nanopore and Nanochannel Transport Studies
Friedrich-Alexander-Universität Erlangen-Nürnberg
2015-2020
Atomistic molecular dynamics simulations have become an important source of information for the structure and biomembranes at detail difficult to access in experiments. A number force fields lipid membrane been derived past; choice most suitable field is, however, frequently hampered by availability parameters specific lipids. Additionally, comparison different quantities among is often aggravated varying simulation parameters. Here, we compare four atomistic fields, namely, united-atom...
Store-operated calcium entry (SOCE) through STIM-gated ORAI channels governs vital cellular functions. In this context, SOCE controls redox signaling and is itself regulated by modifications. However, the molecular mechanisms underlying calcium-redox interplay functional outcomes are not fully understood. Here, we examine role of STIM2 in regulation. Redox proteomics identify cysteine 313 as main sensor vitro vivo. Oxidative stress suppresses currents cells overexpressing ORAI1, an effect...
Biological cells are enveloped by a heterogeneous lipid bilayer that prevents the uncontrolled exchange of substances between cell interior and its environment. In particular, membranes act as continuous barrier for salt macromolecules to ensure proper physiological functions within cell. However, it has been shown membrane permeability strongly depends on temperature and, phospholipid bilayers, displays maximum at transition gel fluid phase. Here, extensive molecular dynamics simulations...
A molecular description of the lipid-protein interactions underlying adsorption proteins to membranes is crucial for understanding, example, specificity or binding strength a protein bilayer, characterizing protein-induced changes membrane properties. In this paper, we extend an automated in silico assay (DAFT) studies and apply it characterize model fusion peptides E K mixed phospholipid/cholesterol using coarse-grained dynamics simulations. addition, couple protocol reverse transformation...
Two-pore channels (TPCs) constitute a family of intracellular cation with diverse permeation properties and functions in animals plants. In the model plant Arabidopsis, vacuolar channel TPC1 is involved propagation calcium waves homeostasis. Here, we discovered that dimerization predicted helix within carboxyl-terminus (CTH) essential for activity TPC1. Bimolecular fluorescence complementation co-immunoprecipitation demonstrated interaction two C-termini pointed towards involvement CTH this...
Lipids and proteins, as essential components of biological cell membranes, exhibit a significant degree freedom for different kinds motions including lateral long-range mobility. Due to their interactions, they not only preserve the cellular membrane but also contribute many important functions e.g. signal transport or molecular exchange with its surrounding. Many these processes take place on short time (up some nanoseconds) length scale nanometers) which is perfectly accessible by...