A5051348706- Botanical Research and Applications
- bioluminescence and chemiluminescence research
- Crystallization and Solubility Studies
- Chemical synthesis and alkaloids
- X-ray Diffraction in Crystallography
- Enzyme Catalysis and Immobilization
- Biocrusts and Microbial Ecology
- Microbial Metabolic Engineering and Bioproduction
- Olfactory and Sensory Function Studies
- Free Radicals and Antioxidants
- Dye analysis and toxicity
- Photodynamic Therapy Research Studies
- Crystallography and molecular interactions
- Antioxidant Activity and Oxidative Stress
- Synthesis and biological activity
- Photoreceptor and optogenetics research
- Bee Products Chemical Analysis
- Electrochemical Analysis and Applications
- Electron Spin Resonance Studies
- Plant and animal studies
- Multiple Myeloma Research and Treatments
- Ferroptosis and cancer prognosis
- Psidium guajava Extracts and Applications
- Chemokine receptors and signaling
- Nanoplatforms for cancer theranostics
Institut de Chimie de Nice
2020-2025
Université Côte d'Azur
2020-2025
Centre National de la Recherche Scientifique
2023-2025
Hôpital Saint Roch
2024
Universidade de São Paulo
2020-2023
Yale University
2017-2023
Observatoire de la Côte d’Azur
2021
TU Wien
2014-2019
Universidade Federal do ABC
2011-2019
Yale Cancer Center
2018
Abstract Background In clear cell renal carcinoma (ccRCC), first-line treatment combines nivolumab (anti-PD-1) and ipilimumab (anti-CTLA4), yielding long-term remissions but with only a 40% success rate. Our study explored the potential of enhancing ccRCC by concurrently using CXCR2 inhibitors alongside immunotherapies. Methods We analyzed ELR + CXCL levels their correlation patient survival during immunotherapy. RCT001, unique inhibitor, was examined for its mechanism action, particularly...
Betanin is a natural pigment with antioxidant properties used as food colourant. This work describes the spectrophotometric and chromatographic quantification of betanin (2S/15S) its epimer isobetanin (2S/15R) in fresh beetroot juice, food-grade powder standard diluted dextrin. Absorption spectra all three samples were deconvoluted using mixed three-function model. Food-grade has largest amount violet-red impurities, probably formed during processing. The purification from these complex...
The decomposition of betanin under dielectric heating (microwave irradiation, power: 25–200 W (3–24 kJ g−1)) follows first-order kinetics with a rate constant similar to that obtained during conventional conduction (half-life < 2 min at 100 °C). Color coordinate analysis indicates is bleached upon thermal treatment, whereas beetroot juice and spray-dried powder tend form colored products. antiradical capacity decreases heating, but still much higher than standard antioxidants such as...
The red pigments of beets are used to produce BeetBlue, a biocompatible blue dye.
UV-like DNA damage is created in the dark by chemiexcitation, which UV-activated enzymes generate reactive oxygen and nitrogen species that create a dioxetane on melanin. Thermal cleavage creates an electronically excited triplet-state carbonyl whose high energy transfers to DNA. Screening natural compounds for ability quench this identified polyenes, polyphenols, mycosporine-like amino acids, related better known as antioxidants. To eliminate false positives such ROS RNS scavengers, we then...
In DNA, electron excitation allows adjacent pyrimidine bases to dimerize by [2 + 2] cycloaddition, creating chemically stable but lethal and mutagenic cyclobutane dimers (CPDs). The usual cause is ultraviolet radiation. Alternatively, CPDs can be made in the dark (dCPDs) via mediated of skin pigment melanin, after it oxidized peroxynitrite formed from stress-induced radicals superoxide nitric oxide. We now show that process not limited unusual structural molecule melanin: signaling...
In this proof-of-concept study, we describe the use of main red beet pigment betanin for quantification calcium dipicolinate in bacterial spores, including Bacillus anthracis. presence europium(III) ions, is converted to a water-soluble, non-luminescent orange 1∶1 complex with stability constant 1.4×105 L mol–1. The addition dipicolinate, largely found changes color aqueous solution [Eu(Bn)+] from magenta. limit detection (LOD) around 2.0×10–6 mol L–1 and LOD determined both B. cereus...
A model betalainic dye was semisynthesized from betanin, the magenta pigment of red beet, and effective for live-cell imaging Plasmodium-infected blood cells. This water-soluble fluorescent probe is photostable, excitable in visible region cell membrane-permeable, its photophysical properties are not notably pH-sensitive. Fluorescence microscopy erythrocytes infected with Plasmodium falciparum, a causative agent malaria humans, showed that only parasite stained. Z-stacking analysis suggested...
The typically low thermodynamic and kinetic stability of enzymes is a bottleneck for their application in industrial synthesis. Baeyer-Villiger monooxygenases, which oxidize ketones to lactones using aerial oxygen, among other activities, suffer particularly from these instabilities. Previous efforts protein engineering have increased but at the price decreased activity. Here, we solved this trade-off by introducing mutations cyclohexanone monooxygenase Acinetobacter sp., guided combination...
Abstract This paper describes the stabilization of flavin‐dependent monooxygenases under reaction conditions, using an engineered formulation additives (the natural cofactors NADPH and FAD, superoxide dismutase catalase as catalytic antioxidants). way, a 10 3 ‐ to 4 ‐fold increase half‐life was reached without resource‐intensive directed evolution or structure‐dependent protein engineering methods. The stabilized enzymes are highly valued for their synthetic potential in biotechnology...
MOPS acts as a Good buffer and electron donor prevents the degradation of catalysts by reactive species in aerated photobiocatalysis.
Many natural processes depend on light energy, which is harnessed by means of photosensitizers. In plants, the photosensitized generation reactive oxygen species part mechanism protection against biotic and abiotic stress. The toxicity this to cells, inspired use photosensitizers in medicine. animal are used phototherapy. This mini-review overviews application therapeutic methods, such as photodynamic therapy cancer noncancerous tissues antimicrobial inactivation compiles their photophysical...
Morpholine derivatives expand the applicability of photobiocatalysis towards stabilization flavin-based bio- and photocatalysts.
Abstract Betalains are phytochemicals of nutraceutical importance that emerged as potent antioxidants, preventing radical chain propagation and the deleterious health effects oxidative stress. However, despite wide application betalains color additives in products for human consumption, little is known about relationship between their structure antioxidant potential. Here we investigate mechanism action three regioisomeric phenolic show meta isomer has higher antiradical capacity than most...
This paper describes the measurement and analysis of in vivo activity stability cyclohexanone monooxygenase from Acinetobacter sp. NCIMB 9871 (CHMO), a model Baeyer-Villiger monooxygenase, recombinant host Escherichia coli. enzyme was often described as poorly stable vitro, has recently been found to deactivate rapidly absence its essential cofactors anti-oxidants. Its scarcely studied, so far. Under conditions common for overexpression CHMO we investigated ability support these properties...
Sulfonylguanidines are interesting bioactive compounds with a broad range of applications in the treatment different pathologies. 2-Aminobenzazole-based structures well employed development new anticancer drugs. Two series novel N-benzazol-2-yl-N'-sulfonyl guanidine derivatives were synthesized sulfonylguanidine either an extra- or intracyclic frame. They evaluated for their antiproliferative activity against malignant melanoma tumor cells, thus allowing structure-activity relationships to...
The diversity of colors found in the flora results from interaction a few classes pigments with light.Betalains are nontoxic vacuolar that replace anthocyanins some families angiosperms and basidiomycete fungi.There two betalains: yellow betaxanthins red betacyanins both biosynthesized betalamic acid, fluorescent aldehyde derivative L-tyrosine.Betalains found, for example, beetroot, dragon fruit, fly agaric, bougainvillea amaranth.In addition, petals varieties four-o'clock eleven-o'clock...
Betaxanthins are natural products with high antioxidant and anti-inflammatory properties. Here, we describe the semisynthesis of twenty-one betaxanthins derived from proteinogenic amino acids, including elusive betaxanthin l-cysteine two l-lysine, rationalize their properties in mechanistic terms. The capacity redox potential these were compared to those model dopamine, l-DOPA (L-3,4-dihydroxyphenylalanine), pyrrolidine structure-property relationships established by using matched molecular...
A diversidade de cores encontradas na flora resulta da interacao algumas poucas classes pigmentos com a luz. Betalainas sao vacuolares e atoxicos que substituem as antocianinas em familias angiospermas alguns fungos basidiomicetos. Existem duas betalainas: betaxantinas amarelas betacianinas vermelhas, ambas biossintetizadas partir um aldeido fluorescente derivado L-tirosina chamado acido betalâmico. encontradas, por exemplo, beterrabas, pitaias, no agario das moscas, primavera amaranto. Alem...