A5007084558- Autophagy in Disease and Therapy
- Biochemical and Molecular Research
- Histone Deacetylase Inhibitors Research
- Click Chemistry and Applications
- Cell death mechanisms and regulation
- Acute Myeloid Leukemia Research
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Immune cells in cancer
- Protein Degradation and Inhibitors
- Chronic Lymphocytic Leukemia Research
- Epigenetics and DNA Methylation
- Sirtuins and Resveratrol in Medicine
- Liver physiology and pathology
- Nuclear Receptors and Signaling
- Endoplasmic Reticulum Stress and Disease
- Chronic Myeloid Leukemia Treatments
- Pancreatic function and diabetes
- Multiple Myeloma Research and Treatments
- Phagocytosis and Immune Regulation
- HIV/AIDS drug development and treatment
- Peptidase Inhibition and Analysis
- Cancer-related molecular mechanisms research
- Inflammasome and immune disorders
Inserm
2015-2024
Université Côte d'Azur
2011-2024
Institut de Recherche sur le Cancer et le Vieillissement de Nice
2021-2024
Centre National de la Recherche Scientifique
2021-2024
Centre Méditerranéen de Médecine Moléculaire
2009-2022
Centre Antoine Lacassagne
2022
Scientific Centre of Monaco
2022
International University of Monaco
2022
Fondation de France
2016-2017
Hôpital l'Archet
2017
Autophagy that is induced by starvation or cellular stress can enable cancer cell survival sustaining energy homeostasis and eliminating damaged organelles proteins. In response to stress, cells have been reported accumulate the protein p62/SQSTM1 (p62), but its role in regulation of autophagy controversial. Here, we report plant phytoalexin resveratrol (RSV) triggers imatinib-sensitive imatinib-resistant chronic myelogenous leukemia (CML) via JNK-dependent accumulation p62. JNK inhibition...
Metformin is the most widely used antidiabetic drug because of its proven efficacy and limited secondary effects. Interestingly, recent studies have reported that metformin can block growth different tumor types. Here, we show exerts antiproliferative effects on melanoma cells, whereas normal human melanocytes are resistant to these metformin-induced To better understand basis this effect in melanoma, characterized sequence events underlying action. We showed 24 h treatment induced a cell...
Abstract CSF-1 and IL-34 share the receptor no differences have been reported in signaling pathways triggered by both ligands human monocytes. promotes differentiation survival of monocytes, macrophages osteoclasts, as does. However, binds other receptors, suggesting that exist effect cytokines. In present study, we compared polarization abilities primary monocytes response to or IL-34. CSF-1R engagement one leads AKT caspase activation autophagy induction through expression AMPK ULK1. As...
Ischemia-reperfusion (IR) injury induces endoplasmic reticulum (ER) stress and cell death. Bax Inhibitor-1 (BI-1) is an evolutionarily conserved ER protein that suppresses death abundantly expressed in both liver kidney. We explored the role of BI-1 protection from IR by using bi - 1 knockout mice, employing models transient hepatic or renal artery occlusion. Compared to wild-type bi-1 mice subjected exhibited these characteristics: ( i ) increased histological injury; ii serum...
Autophagy is induced during differentiation of human monocytes into macrophages that mediated by CSF1/CSF-1/M-CSF (colony stimulating factor 1 [macrophage]). However, little known about the molecular mechanisms link CSF1 receptor engagement to induction autophagy. Here we show CAMKK2-PRKAA1-ULK1 pathway required for CSF1-induced autophagy and monocyte differentiation. We reveal this links P2RY6 autophagy, decipher signaling network P2RY6-mediated In addition, physiological ligand UDP...
Endoplasmic reticulum (ER) stress is activated in nonalcoholic fatty liver disease (NAFLD), raising the possibility that ER stress‐dependent metabolic dysfunction, inflammation, and cell death underlie transition from steatosis to steatohepatitis (nonalcoholic steatohepatitis; NASH). B‐cell lymphoma 2 (BCL2)‐associated X protein (Bax) inhibitor‐1 (BI‐1), a negative regulator of sensor, inositol‐requiring enzyme 1 alpha (IRE1α), has yet be explored NAFLD as hepatoprotective agent. We...
Abstract Background In clear cell renal carcinoma (ccRCC), first-line treatment combines nivolumab (anti-PD-1) and ipilimumab (anti-CTLA4), yielding long-term remissions but with only a 40% success rate. Our study explored the potential of enhancing ccRCC by concurrently using CXCR2 inhibitors alongside immunotherapies. Methods We analyzed ELR + CXCL levels their correlation patient survival during immunotherapy. RCT001, unique inhibitor, was examined for its mechanism action, particularly...
Recently, we discovered that Humanin (HN), a small endogenous peptide of 24 amino acids, binds to and inhibits the proapoptotic protein Bax. We show here HN also interacts with BH3-only Bcl-2/Bax family protein, Bid, as well truncated form Bid (tBid) associated protease-mediated activation this protein. Synthetic purified tBid in vitro blocks tBid-induced release cytochrome c SMAC from isolated mitochondria, whereas mutant peptides fail bind or lack activity. Moreover, retained protective...
Maeva Dufies 1,2,3 , Arnaud Jacquel Nathalie Belhacene Guillaume Robert Thomas Cluzeau 1,2,3,4 Fréderic Luciano Jill Patrice Cassuto 2,4 Sophie Raynaud 2,5 and Patrick Auberger 1 INSERM U895, Centre Méditerranéen de Médecine Moléculaire, Team «Cell Death, Differentiation, Inflammation Cancer», Nice, France 2 Université Nice Sophia Antipolis, Faculté Médecine, 3 Equipe labellisée par la Ligue Nationale Contre le Cancer 2011-2013, Paris, 4 Service d'Hématologie Clinique et Transplantation, 5...
Azacitidine is the leading compound to treat patients suffering myelodysplastic syndrome (MDS) or AML with less than 30% of blasts, but a majority primary refractory rapidly relapses under treatment. These have drastically reduced life expectancy as compared sensitive patients. Therefore identifying predictive factors for AZA resistance great interest propose alternative therapeutic strategies non-responsive We generated AZA-resistant myeloid cell line (SKM1-R) that exhibited increased...
Acute myeloid leukemia cells are dependent on the DNA repair function of valosin-containing protein, opening up an avenue for disease treatment.
Imatinib has emerged as the lead compound for clinical development against chronic myelogenous leukemia (CML). induces apoptosis of CML cells, but how drug kills them is only partially understood. This study was conducted to 1) analyze effect imatinib on and differentiation Bcr-Abl-positive K562 cell line 2) determine influence that have each other in this appropriate model. A 36 h exposure cells 1 µM induced DNA fragmentation (Fig. 1A), indicating underwent an apoptotic program presence...
Humanin (HN) is a recently identified endogenous peptide that protects cells against cytotoxicity induced by various stimuli. Recently, we showed HN binds to and inhibits Bax, proapoptotic Bcl-2 family protein, suggesting mechanism for action. In this study, Bim, homology 3-only member of the Bcl-2/Bax family, as an additional target protein. Using in vitro protein binding, immunoprecipitation, coimmunolocalization assays, demonstrated directly extra long isoform Bim (BimEL) but not (BimL)...
Autophagy is a highly conserved catabolic process for the elimination and recycling of organelles macromolecules, characterized by formation double membrane vesicles called autophagosomes. To date, function autophagy in cell differentiation poorly documented. Here, we investigated possibility that megakaryocytic Chronic Myelogenous Leukemia (CML) line K562, known to be accompanied accumulation vacuoles inside cells, might involve autophagy. We show using various complementary approaches...