This study was designed to examine whether mesenchymal stem cells (MSCs) transduced with Akt enhance cardiac repair after transplantation into the ischemic porcine heart.MSCs isolated from bone marrow and myr-Akt were transplanted hearts experimental myocardial infarction (MI) using intracoronary injection [Group I, vehicle; Group II, MSCs; III, Akt-MSCs]. Myocardial single photon emission tomography (M-SPECT) performed assess function infarcted area. Pigs also sacrificed for...

Extracorporeal shock wave therapy (ESWT) considerably improves the appearance and symptoms of post-burn hypertrophic scars (HTS). However, mechanism underlying observed beneficial effects is not well understood. The objective this study was to elucidate changes in cellular molecular biology that induced by ESWT fibroblasts derived from scar tissue (HTSFs). We cultured primary dermal human HTS exposed these cells 1000 impulses 0.03, 0.1, 0.3 mJ/mm2. At 24 h 72 after treatment, real-time PCR...

Epigallocatechin-3-gallate (EGCG), the primary catechin in green tea, has anti-inflammatory and anti-oxidative properties. The aim of current study was to characterize impact EGCG on lipopolysaccharide (LPS)-induced innate signaling bone marrow-derived macrophages (BMMs) isolated from ICR mice.The effect LPS-induced pro-inflammatory gene expression nuclear factor-κB (NF-κB) mitogen-activated protein kinase (MAPK) examined using reverse transcription-polymerase chain reaction, Western...

Abstract Septic arthritis (SA) are rare in patients with burns, but delayed treatment can result irreversible joint destruction. Early diagnosis and immediate necessary to prevent Robot training musculoskeletal diseases improve range of motion (ROM), muscle strength, lower extremity function. The aim this report is present robot utility patient electrical burns associated SA. Rebless® (H-ROBOTICS, KOREA) for ROM strength training, operate passive or active modes knee ankle flexion extension....

The hyomandibular (HM) cartilage securing the lower jaw to neurocranium in fish is a craniofacial skeletal element whose shape and function have changed dramatically vertebrate evolution, yet genetic mechanisms shaping this are less understood. Using mutants rescue experiments zebrafish, we reveal previously unappreciated role of Pax1a anterior HM plate formation through EphrinB2a. During development, pax1a expressed pharyngeal endoderm from segmentation stage chondrocyte formation. Loss...

We describe our experience with the 'four-in-one' procedure for habitual dislocation of patella in five children (six knees). All patients presented severe generalised ligamentous laxity and aplasia trochlear groove. had a lateral release, proximal 'tube' realignment patella, semitendinosus tenodesis transfer patellar tendon. The mean age at time operation was 6.1 years (4.9 to 6.9), were followed up 54.5 months (31 66). clinical results evaluated using Kujala score. There has been no...

Hands are the most frequent burn injury sites. Appropriate rehabilitation is essential to ensure good functional recovery. Virtual reality (VR)-based has proven be beneficial for recovery of upper extremities. We investigated and compared VR-based with conventional (CON) in patients burned hands. Fifty-seven were randomized into a VR or CON group. Each intervention was applied affected hand four weeks, clinical variables evaluated. Hand function evaluated before weeks after using...

The application of three-dimensional (3D) printing is growing explosively in the medical field, and especially widespread clinical use fabricating upper limb orthosis prosthesis. Advantages 3D-printed compared to conventional ones include its lower cost, easier modification, faster fabrication. Hands are most common body parts involved with burn victims one main complications hand burns finger joint contractures. Applying orthotic devices such as splints a well-established essential element...

Background: Extracorporeal shock wave therapy (ESWT) has been used to reduce pain in patients with various musculoskeletal diseases and wounds. We investigated the effect of ESWT on scar after complete wound epithelialization burn patients. Methods: A prospective, single-blind, placebo-controlled study was conducted from February 2014 2015. Forty despite standard (medication, physical therapy, rehabilitation massage therapy) were randomized into or control (sham ESWT) groups. administered at...

Epidermal keratinocytes are highly activated, hyper-proliferated, and abnormally differentiated in the post-burn hypertrophic scar (HTS); however, effects of fibroblasts (SFs) on through cell-cell interaction HTS remain unknown. Here, we investigated HTSF-derived exosomes proliferation differentiation normal human (NHKs) compared with (NFs) their possible mechanism to provide a reference for clinical intervention HTS. Fibroblasts were isolated cultured from skin. Both HTSF-exosomes...

Post-burn hypertrophic scars often exhibit abnormal pigmentation. Exosomes play important roles in maintaining normal physiological homeostasis and the pathological development of diseases. This study investigated effects exosomes derived from scar fibroblasts (HTSFs) on melanocytes, which are pigment-producing cells. Normal (NFs) HTSFs were isolated cultured skin (HTS) tissue. Both NF- HTSF-exosomes a cell culture medium purified using column-based technique. The human epidermal melanocytes...

Abstract Burn injuries often result in severe hand complications, including joint contractures and nerve damage, sometimes leading to amputation. Despite early treatment, hypertrophic scarring frequently hampers function recovery, the thick raised scar blocks electromyography (EMG) sensing. A promising solution involves motion-mimicking robotic finger prostheses tailored individual patient requirements. By using versatility of motion-capturing technology on a sound finger, prosthesis can...

Human skin incurs damage in various ways; a few of the recognizable factors are electric burns, chemical scalding surgery, and physical injuries, such as automobile accidents. If is extensive severe enough [second degree total body surface area (TBSA) involvement >20%], will lose ability to regenerate itself. Burn wounds treated ways. For full-thickness defects, mainstay treatment an autologous split-thickness graft. However, there some limitations due scars, poor elasticity, joint movements...

Burn injuries and their treatment are extremely painful. This study aimed to determine whether virtual reality (VR) could reduce pain during robot-assisted gait training (RAGT) in burn patients by analyzing the cerebral blood flow (CBF) prefrontal cortex over time using functional near-infrared spectroscopy (fNIRS). The included this complained of a score ≥5 on visual analog scale (VAS) RAGT, which was performed 10 times for 2 weeks. Each session consisted 15 min VR application, with 2-min...

Background. The aldehyde products of lipid peroxidation such as 4-hydroxy-2-hexenal (HHE) might be responsible for the pathogenesis kidney injury. present study was aimed to investigate effects HHE on renal tubular epithelial cells and its signaling mechanisms. Methods. Human proximal (HK-2) were treated with 10 μM HHE. Cell viability examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. fluorescent probe 2′,7′-dichlorofluorescein diacetate used measure...

To investigate effects of tracheostomy tube on the movement hyoid bone and larynx during swallowing by quantitative analysis videofluoroscopic study.19 adult stroke patients with tracheostomies, who met criteria decannulation participated. Serial videofluroscopic studies were done over 14 days before decannulation, within 24 hours after decannulation. The kinematic parameter such as pharyngeal transition time, stage duration, maximal movement, laryngeal prominence obtained 2-D...