A5017621937- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- Viral Infections and Vectors
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Milk Quality and Mastitis in Dairy Cows
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Insect Pest Control Strategies
- T-cell and Retrovirus Studies
- Hepatitis Viruses Studies and Epidemiology
- Streptococcal Infections and Treatments
- Water Treatment and Disinfection
- Metabolism, Diabetes, and Cancer
- Hepatitis B Virus Studies
- Research on Leishmaniasis Studies
- Virus-based gene therapy research
- Infectious Diseases and Mycology
- Veterinary Oncology Research
- Coccidia and coccidiosis research
- Livestock and Poultry Management
- Diet, Metabolism, and Disease
University of Veterinary and Animal Sciences
2016-2025
Iowa State University
2014-2025
King Edward Medical University
2023
Abstract Hepatitis E is a global health concern. virus (HEV) infection endemic in Pakistan. HEV has four genotypes: HEV-1 through HEV-4. The genotypes and HEV-2 are associated with humans, especially countries poor sanitation. - 3 HEV-4 zoonotic human takes place by consuming undercooked meat or being contact animals. present study was designed to ascertain the presence of Southern Punjab region First, blood samples (n = 50) were collected from patients suspected hepatitis Multan District....
Lactuca sativa have been shown to the potential act as antidiabetic drugs, using diabetic rats subjects in research that are currently being conducted. In treatment of hyperglycemia and prevention metabolic problems associated with diabetes, these herbs be effective. The utilization for diabetes has employed a considerable amount time, findings lend credence approach. One possible reason reported effects could Lactucasativa can enhance activity pancreatic β-cells islets Langerhans increase...
Influenza A virus (IAV) surveillance using pre-weaning oral fluid samples from litters of piglets was evaluated in four ˜12 500 sow and IAV-vaccinated, breeding herds. Oral were collected 600 serum their dams at weaning. Litter tested for IAV by isolation, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), RT-PCR subtyping sequencing. Commercial nucleoprotein (NP) enzyme-linked immunosorbent assay (ELISA) kits NP isotype-specific assays (IgM, IgA IgG) used to...
Reports of a mass die-off ∼350 elephants (Loxodonta africana) in northern Botswana over period two months (May–June 2020), has fuelled speculation and concern regarding the cause. Although area which these mortalities occurred is not protected considered hotspot for human–elephant conflict poaching, both malicious poisoning poaching are unlikely to have played role as other species were affected, elephant carcasses found with tusks intact. In absence confirmed cause we sought identify lines...
Background Avian influenza virus (AIV) infections occur naturally in wild bird populations and can cross the wildlife-domestic animal interface, often with devastating impacts on commercial poultry. Migratory waterfowl shorebirds are natural AIV reservoirs carry along migratory pathways, without exhibiting clinical signs. However, these species rarely inhabit poultry farms, so transmission into domestic birds likely occurs through other means. In many cases, human activities thought to...
Repeated cases of low pathogenic influenza A/H9N2 virus (IAV/H9N2) have been reported in commercial chickens since its emergence 1998 Pakistan. However, recently increased mortality and severe respiratory complications under field conditions noticed, suggesting concomitant infections with viral and/or bacterial pathogens. Therefore, the present study aimed to investigate presence IAV/H9N2 coinfecting multiple pathogens broiler chicken flocks. We surveyed 60 flocks signs from March through...
The present study was designed to evaluate the utility of environmental samples for convenient but accurate detection avian influenza virus (AIV) in commercial poultry houses. First, from AIV-negative layer facilities were spiked with an H5N2 low pathogenic AIV and evaluated their effect on viral RNA immediately or after incubation at -20 C, 4 22 37 C 24, 48, 72 hr. Second, Swiffer pads, drag swabs, boot cover swabs efficiency collecting feces water LPAIV under a condition simulated facility...
The present study evaluated the potential utility of feather samples for convenient and accurate detection avian influenza virus (AIV) in commercial poultry. Feather were obtained from AIV-negative layer facilities Iowa, USA. feathers spiked with various concentrations (106 to 100) a low pathogenic strain H5N2 AIV using nebulizing device viral RNA real-time RT-PCR assay immediately or after incubation at -20, 4, 22, 37 °C 24, 48, 72 h. Likewise, cell culture medium without prepared used...
A Sahiwal breed heifer calf of ~ 5-month age was presented with numerous warts varying sizes principally on head, face and neck. The history clinical examination were suggestive bovine cutaneous papillomatosis. animal treated medically an alternative medicine: Thuja occidentalis 30X. regressed completely after two months treatment occidentalis, suggesting this medicine can be used to treat in bovines successfully.
The surveillance of migratory waterbirds (MWs) for avian influenza virus (AIV) is indispensable the early detection a potential AIV incursion into poultry. Surveying infections and subtypes in understudied MW species could elucidate their role ecology. Oropharyngeal-cloacal (OPC) swabs were collected from non-mallard MWs between 2006 2011. OPC (n = 1158) that molecularly tested positive (Cts ≤ 32) but negative H5 H7 selected isolation (VI). samples evenly represented birds all four North...
Pure, potent and efficacious vaccines could help in the control of Newcastle disease (ND). The present study was designed to evaluate thermo-stability a live-attenuated ND virus vaccine containing Mukteswar strain genetically characterize seed virus. Moreover, presence extraneous agents (Fowl adenovirus, Mycoplasma, Salmonella Pullorum, Gallinarum) assessed using polymerase chain reactions (PCR) optimized for detection panel. evaluated its potency efficacy after storage at 4°C, 25°C 37°C 36,...
Trypanosoma evansi is an important hemoparasite of a variety animal species worldwide. This parasite threat to the health domestic animals as well wild animals, particularly those managed in captivity. The current study investigated presence T. captive tigers (Panthera tigris tigris) and lions leo) Pakistan. In total, 24 blood samples from 11 3 (n = 14) were collected during course roughly yr (2016-2018). Eighteen subjected both microscopic molecular evaluation for evansi; remaining 6...
The present study was aimed to detect C. perfringens and identify its toxins in mutton samples collected from Lahore City the Punjab Province of Pakistan. A total 40 minced non‑minced were local butcher retail shops representing four areas city. subjected ELISA for detection alpha, beta epsilon toxins. simultaneously processed bacterial isolation. isolates confirmed both by biochemical testing a multiplex PCR targeting toxin genes perfringens. While 10% (4/40) positive alpha toxins, 17.5%...
The present study was conducted to assess the potential vector role of feedstuffs for area spreading avian influenza virus (AIV). Firstly, feed samples were collected from commercial poultry facilities that experienced highly pathogenic (H5N2) in 2014−2015 AIV testing by a real-time RT−PCR specific viral matrix gene. Secondly, materials obtained an AIV-negative farm spiked with various concentrations low H5N2. Virus-spiked cell culture media prepared same manner and used comparison. tested...
Eight essential oils: Zingiber officinale, Curcuma longa, Eucalyptus globulus, Syzygium aromaticum, Nigella sativa, Elettaria cardamomum, Cinnamomum verum and Cuminum cyminum were evaluated for antifungal activity against Ochratoxin A (OTA)-producing Aspergillus parasiticus (n = 3) using grains as a substrate.Minimum inhibitory concentration (MIC) log reduction assay used to evaluate the activity.Both highest zone of inhibition (24.00±2.00mm) least minimum (1.04±0.45µg/mL) recorded C....
The therapeutic potency of plants has been known for ages and the ability to divulge their biological activity is an area great interest.Citrus lemon traditionally used as antioxidant, antibacterial anti-inflammatory agent.This study intended determine potential role peel oil in neurobehavioral function oxidative stress.Rats were treated with at a dose 0.7, 1.4, 2.1, 2.7, 3.5 g/kg 14 days.Results showed that low doses antidepressant anxiolytic activity.Muscular strength was also improved...
Thirty puppies were randomly divided in to 3 groups for comparative evaluation of two commercial CPV vaccines. Each group was further subdivided < 6 months and -old either vaccinated with a monovalent vaccine: Primodog, combination Duramune or maintained as non-vaccinated control. Humoral immune response determined by Hemagglutination Inhibition (HAI) on 21 35 -days after vaccination. The geometric mean titer (GMT) induced Duramune, post-vaccination GMT 73.3 137.2, respectively....
Finding alternate ways to relieve animals from the ectoparasites is one of most important aspects under One-Health concept. Herbal ectoparasiticides offer a promising chemical ectoparasiticides. The present study was designed evaluate ectoparasiticide efficacy Nimboliâ (Neem: Azadirachta indica extract) in various animal species, keeping view objectives One-Health. also compared with commercial Tagafon (Hydroxyethyl Phosporic Acid). Various infested ticks, fleas, lice, flies, and maggots...