A5033896936- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Biochemical effects in animals
- Neurotransmitter Receptor Influence on Behavior
- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- GABA and Rice Research
- Diet, Metabolism, and Disease
- Eicosanoids and Hypertension Pharmacology
- Pharmacological Receptor Mechanisms and Effects
- Receptor Mechanisms and Signaling
- Biochemical Acid Research Studies
- Neurological Disease Mechanisms and Treatments
- Sleep and Wakefulness Research
- Parkinson's Disease Mechanisms and Treatments
- Free Radicals and Antioxidants
- Biochemical Analysis and Sensing Techniques
- Lipid metabolism and biosynthesis
- Fatty Acid Research and Health
- Sphingolipid Metabolism and Signaling
- Cholesterol and Lipid Metabolism
- Synthesis and biological activity
- Porphyrin and Phthalocyanine Chemistry
- Forensic Toxicology and Drug Analysis
- Nuclear Receptors and Signaling
Institute of Bioorganic Chemistry
2016-2025
Institute of Bioorganic Chemistry
2022-2024
S.P.E.C.I.E.S.
2022
Russian Academy of Sciences
2004-2015
Umeå University
2002-2006
Institute of Molecular Genetics
1988
We reported previously that synthetic amides of polyunsaturated fatty acids with bioactive amines can result in substances interact proteins the endogenous cannabinoid system (ECS). Here we synthesized a series N-acyl-dopamines (NADAs) and studied their effects on anandamide membrane transporter, amidohydrolase (fatty acid amide hydrolase, FAAH) two receptor subtypes, CB(1) CB(2). NADAs competitively inhibited FAAH from N18TG2 cells (IC(50)=19-100 microM), as well binding selective ligand,...
2-arachnadoyl glycerol (2-AG) is one of the most common endocannabinoid molecules with anti-proliferative, cytotoxic, and pro-proliferative effects on different types tumors. Typically, it induces cell death via cannabinoid receptor 1/2 (CB1/CB2)-linked ceramide production. In breast cancer, counterbalanced by sphingosine-1-phosphate, thus mechanisms 2-AG influence proliferation are poorly understood. We evaluated mechanism anti-proliferative action lysophaosphatidylinositol (LPI) in six...
Relevance. The problem of prevention and treatment influenza remains relevant due to the consistently high incidence rate disease in population all age groups, especially children elderly. Antiviral drugs have become most important tools fight against viruses. main is search development new antiviral that do not side effects. aim present study investigate effect a synthetic drug candidate based on adamantane (NMG 1110) its combination with ribavirin reproduction A virus MDCK cell culture....
We reported previously that synthetic amides of polyunsaturated fatty acids with bioactive amines can result in substances interact proteins the endogenous cannabinoid system (ECS). Here we synthesized a series N-acyl-dopamines (NADAs) and studied their effects on anandamide membrane transporter, amidohydrolase (fatty acid amide hydrolase, FAAH) two receptor subtypes, CB1 CB2. NADAs competitively inhibited FAAH from N18TG2 cells (IC50 = 19–100µM), as well binding selective ligand,...
Numerous derivatives of BODIPY (4,4-difluoro-4-borata-3a-azonia-4a-aza-s-indacene) are frequently used as fluorescent probes in modern protein and lipid research. Present studies purpose-synthesised molecules show that the chromophore can form two different ground state dimers, denoted DI DII. In practise exhibits negligible fluorescence emission, but a strong absorption band {ε(477 nm) = 102 000 mol−1 dm3 cm−1}, while DII is with radiative lifetime 20 ns, red-shifted {ε (577 nm)} 26 cm−1}...
Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While inhibits the of cells, LPI is known as stimulant. Despite endocannabinoid receptor crosstalk simultaneous presence microenvironment both molecules, their combined activity has never been studied. We evaluated effect on AEA six human breast lines different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin...
We demonstrate that the ganglioside GM1 in lipid bilayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) exhibits a non-uniform lateral distribution, i.e., enriched regions molecules are formed, which is an argument favour self-aggregation being intrinsic property ganglioside. This was concluded from energy transfer/migration studies BODIPY-labelled gangliosides by means time-resolved fluorescence lifetime and depolarization experiments. Three fluorophore-labelled were synthesized to...
Cholines acylated with unsaturated fatty acids are a recently discovered family of endogenous lipids. However, the data on biological activity acylcholines remain very limited. We hypothesized that containing residues arachidonic (AA-CHOL), oleic (Ol-CHOL), linoleic (Ln-CHOL), and docosahexaenoic (DHA-CHOL) act as modulators acetylcholine signaling system. In radioligand binding assay, showed inhibition in micromolar range both α7 neuronal nAChR overexpressed GH4C1 cells muscle type from...
Abstract N ‐acyl dopamines (NADAs) are bioactive lipids of the endovanilloid family with known cytotoxicity for cancer cells; however, available data on participation endovanilloids in epithelial–mesenchymal transition (EMT) and stemness controversial. This study unveils inhibitory role ‐arachidonoyl dopamine (AA‐DA), a typical representative NADA family, breast cell migration, EMT, stemness. AA‐DA treatment also led to decrease cholesterol biosynthesis gene expressions, addition exogenous...
GPR55 is a non-canonical cannabinoid receptor, important for cancer proliferation. Depending on the ligand, it induces either cell proliferation or death. The objective of study was to establish mechanisms this multidirectional signaling. Using CRISPR-Cas9 system, GPR55, CB1, CB2, and GPR18 receptor knockouts MDA-MB-231 line were obtained. After CB2 knockout, pro-apoptotic activity ligand docosahexaenoyl dopamine (DHA-DA) slightly increased, while pro-proliferative most active synthetic...
Plasma membranes of living cells are compartmentalized into small submicroscopic structures (nanodomains) having potentially relevant biological functions. Despite this, structural features these nanodomains remain elusive, primarily due to the difficulties in characterizing such dynamic entities. It is unclear whether found upper bilayer leaflet transversally registered with those lower leaflet. Experiments performed on larger microscopic domains indicate that coupling between leaflets...
GPR55 is a GPCR of the non-CB1/CB2 cannabinoid receptor family, which activated by lysophosphatidylinositol (LPI) and stimulates proliferation cancer cells. Anandamide, bioactive lipid endocannabinoid, acts as biased agonist induces cell death, but unstable psychoactive. We hypothesized that other endocannabinoids structurally similar compounds, are more hydrolytically stable, could also induce death via activation. chemically synthesized tested set fatty acid amides esters for induction The...
In this paper we have investigated the behaviour of newly synthesised mono-palmitoyl- and dipalmitoyl-phosphatidylethanolamine probes (abbreviated as mPE dPE, respectively) labelled in polar headgroup region by either FL-BODIPY or 564/570-BODIPY fluorophore solubilised lipid systems that exhibit different curvatures. Because bulky BODIPY-groups, monoacyl-form derivatives a conic-like shape, whereas for diacyl is rather cylindrical. A careful analysis time-resolved resonance energy transfer...