Cardiac fibrosis, as a pathological process, plays an important role in various cardiac diseases. microRNA-155 (miR-155) is one of the most miRNAs, and previous studies have shown that it regulatory factor fibrotic However, mechanism by which miR-155 affects myocardial fibrosis remains unclear. In this study, we aim to establish biological function induced diabetes mice. We used normal C57BL/6 wild type (WT) knockout (KO) mice diabetic model intraperitoneal injection streptozotocin, utilized...

Background Chemoimmunotherapy is the first-line therapy for patients with recurrent or metastatic nasopharyngeal carcinoma (NPC) and currently main induction treatment option locoregionally advanced NPC. However, it remains unclear whether combining immunotherapy standard chemotherapy enhances its efficacy. This study aimed to evaluate efficacy, toxicity, survival outcomes of chemoimmunotherapy in

Surgical intervention is the main treatment for a ruptured congenital sinus of Valsalva aneurysm (SVA). However, reports on surgical experience are scarce. We retrospectively analysed cases our centre to summarize 10-year experience.A total 286 patients who were diagnosed with SVA and underwent repair between 2007 2016 identified analysis. Follow-up data (mean ± standard deviation: 49.6 34.9 months) obtained from outpatient department records telephone calls.The SVAs originated right...

REV-ERB agonists have shown antifibrotic effects in the heart and other organs. The function of cardiac fibroblasts remains unstudied. Here, we characterize functional difference mouse embryonic using genetic deletion REV-ERBα ß vitro . We show that α/β double deleted reduced viability proliferation, but increased migration myofibroblasts activation. Thus, has essential cell-autonomous role maintaining them a healthy, quiescent state. also existing agonist SR9009 strongly suppresses...

Abstract Objectives This study was conducted to investigate whether percutaneous closure of ruptured sinus Valsalva aneurysm (SVA) is as safe and effective surgery repair. Background Percutaneous SVA has been becoming an alternative the traditional surgical repair recently. The reports regarding direct comparison these two treatment options are scarce. Methods medical records from institutional database were retrospectively analyzed. A total 134 patients reviewed, including 26 undergoing 108...

The porcine mitral regurgitation (MR) model is a common cardiovascular animal model. Standardized manufacturing processes can improve the uniformity and success rate of model, systematic research evaluate its potential use. In this study, 17 pigs were divided into an experimental group (n=11) control (n=6). We used homemade retractor to cut chordae via left atrial appendage establish MR; underwent sham surgery. animals followed for 30 months after Enlargement fibrosis atrium significant in...

Proton nuclear magnetic resonance ([ 1 H]-NMR) spectroscopy has been used to investigate metabolites in serum and several types of tissue.We NMR explore the differential metabolic profiles from nasopharyngeal carcinoma (NPC) patients.Moreover, with potential as biomarkers for identifying NPC patients were primarily identified.Serum samples collected 40 enrolled participants comprising 20 healthy subjects patients.Samples analyzed using a 600-MHz spectrometer.The [ H]-NMR spectra further...

Background: The early diagnosis of unilateral absence pulmonary artery (UAPA) in children offers an opportunity for effective intervention. Due to the lack clinical evidence, a consensus regarding surgical treatment has yet be reported. aim this study is evaluate effectiveness and safety (PA) reconstruction with "two-segment" technique repair UAPA patients hypertension.

Pulmonary arterial hypertension (PAH) is a chronic and severe cardiopulmonary disorder. Mice are popular animal model used to mimic this disease. However, the evaluation of right ventricular pressure (RVP) pulmonary artery (PAP) remains technically challenging in mice. RVP PAP more difficult measure than left because anatomical differences between heart systems. In paper, we describe stable hemodynamic measurement method its validation using healthy PAH This based on open-chest surgery...

miR-155 is one of the most important miRNAs and plays a very role in numerous biological processes. However, few studies have characterized this miRNA mice under normal physiological conditions. We aimed to characterize vivo by using comparative analysis. In our study, we compared knockout (KO) with C57BL/6 wild type (WT) order conditions many evaluation methods, including reproductive performance analysis, growth curve, ultrasonic estimation, haematological examination, histopathological...

Postinfarction ventricular septal rupture (VSR) is an uncommon but challenging mechanical complication for surgeons. This study analyzed the impacts of size on surgical outcomes in patients with VSR.During a 15-year period, from January 2006 to December 2020, 112 underwent repairs postinfarction VSR. Patient clinical data, including angiographic and echocardiographic findings, operative procedures, early morbidity mortality, survival time were collated. Univariable multivariable analyses...

Background A "boot camp" program is commonly adopted in surgical skills training. Due to a shortage of cardiac surgeons, establishment well-designed training curriculum for surgery residents needed developing countries. Methods We established comprehensive 3-module curriculum, including: (1) the cardiopulmonary bypass technique, (2) coronary artery anastomosis and (3) basic thoracoscopy. Each module was designed one with time 1 week. included theoretical knowledge learning, demonstration by...

Pulmonary arterial hypertension (PAH) is a chronic and severe cardiopulmonary disorder. Mice are popular animal model used to mimic this disease. However, the evaluation of right ventricular pressure (RVP) pulmonary artery (PAP) remains technically challenging in mice. RVP PAP more difficult measure than left because anatomical differences between heart systems. In paper, we describe stable hemodynamic measurement method its validation using healthy PAH This based on open-chest surgery...

Background: Cardiopulmonary bypass is the basis of open heart surgery. Through simulation-based learning, residents may receive structured training cardiopulmonary techniques. Therefore, we built a high-fidelity, tissue-based simulation model for cannulation/decannulation training. Methods: The core whole block cadaveric animal and lung. discarded membrane oxygenators are used as blood reservoirs. tubing suckers recycled from experiments washed reused. To set up model, lung placed into...

Many countries are facing a shortage of cardiac surgeons, who crucial in meeting the demands growing number patients need coronary artery bypass grafting. This situation poses serious challenge, especially China. The purpose this study is to determine whether surgeons suitable for training anastomosis at an earlier stage their career.We divided 12 with no prior experience into senior and junior groups assessment. All trainees received defined period. We performed vivo vitro examinations...

Objective: To investigate the clinicopathological features, treatment and prognosis of duodenal-type follicular lymphoma. Methods: Four cases lymphoma diagnosed at Guangdong General Hospital from 2014 to 2015 with detailed clinical data were included. The histomorphology, immunophenotype, prognoses analyzed. Results: patients' age ranged 51 57 years (mean 54 years), there 2 males females. involved sites gastric fundus in one case, second portion duodenum two terminal ileum case. All patients...

Objective: In-utero correction is an option for treatment of critical congenital heart diseases (CHDs). Fetal cardiac surgery CHDs dependent on the reliable use fetal cardiopulmonary bypass (CPB), but this technology remains experimental. In study, we established CPB models with central and peripheral cannulation to explore differences between two strategies. Methods: Ten sheep 90–110 gestational days were randomized into ( n = 5) groups. All successfully established. At each time point (0,...

Objective To explore for the construction of microRNA-155 (miR-155) gene knockout mouse model by using CRISPR/Cas9 technique. Methods Healthy C57BL/6J mice were selected. First, target sequence genome was amplified and sequenced to identify miR-155 loci. Then Cas9 vector (Cas9 RNA) targeting single guide RNA (sgRNA) designed constructed. After in-vitro transcription, sgRNA injected into fertilized eggs which cultured in vitro. The qualified embryos transplanted fallopian tubes...

<h3>Background</h3> Alterations in T-lymphocyte homeostasis have been suggested to play a key role the pathogenesis of SLE. Autophagy is now emerging as core player development and functioning immune system. <h3>Objectives</h3> we investigated autophagic behavior T cells from patients with <h3>Methods</h3> Thirty SLE twenty-five healthy subjects matched for gender age were recruited. The levels mRNA encoding ATG5, ATG7, Beclin-1 LC3 was determined by quantitative real-time polymerase chain...

Objective To compare the effects between dual-cycle intensive training mode (DCITM) of teaching-in vitro-in vivo-summary-in vivo-assessment and single-cycle basic (BTM) on graduate students majoring in cardiovascular surgery. Methods Eight surgery received coronary artery bypass vascular anastomosis under BTM or DCITM. Then were evaluated by scoring experts. Besides, SPSS 17.0 statistical software was used to analyze evaluation results. Results Compared with who BTM,...