A5071783406- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Cell death mechanisms and regulation
- Black Holes and Theoretical Physics
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Algebraic structures and combinatorial models
- Phagocytosis and Immune Regulation
- Noncommutative and Quantum Gravity Theories
- PARP inhibition in cancer therapy
- Nuclear physics research studies
- Quantum Chromodynamics and Particle Interactions
- History and Theory of Mathematics
- Nonlinear Waves and Solitons
- Mathematical and Theoretical Analysis
- Synthesis and Characterization of Heterocyclic Compounds
- Particle physics theoretical and experimental studies
- Mathematics and Applications
- Nitrogen and Sulfur Effects on Brassica
- Rare-earth and actinide compounds
- Plant Virus Research Studies
- Genomics, phytochemicals, and oxidative stress
- Mathematics Education and Teaching Techniques
State Key Laboratory of Genetic Engineering
2019-2024
Fudan University
2019-2024
Pudong Medical Center
2023-2024
Shenyang Pharmaceutical University
2022-2024
Hunan Agricultural University
2024
Zhongshan Hospital
2019-2023
University of Chinese Academy of Sciences
2022
Chinese Academy of Sciences
2022
Institute of Theoretical Physics
2022
Wuhan University
2019-2020
For over two decades, the development of B-cell lymphoma-2 (Bcl-2) family therapeutics has primarily focused on anti-apoptotic proteins, resulting in first-in-class drugs called BH3 mimetics, especially for Bcl-2 inhibitor Venetoclax. The pro-apoptotic protein Bcl-2-associated X (BAX) plays a crucial role as executioner mitochondrial regulated cell death, contributing to organismal development, tissue homeostasis, and immunity. dysregulation BAX is closely associated with onset progression...
The compact CRISPR/Cas9 system, which can be delivered with their gRNA and a full-length promoter for expression by single adeno-associated virus (AAV), is promising platform therapeutic applications. We previously identified SauriCas9 that displays high activity requires simple NNGG PAM, but the specificity moderate. Here, we three Cas9 orthologs, Staphylococcus lugdunensis (SlugCas9), lutrae (SlutrCas9) haemolyticus (ShaCas9), mammalian genome editing. Of these SlugCas9 recognizes PAM...
The RNA-guided CRISPR/Cas9 system is a powerful tool for genome editing, but its targeting scope limited by the protospacer-adjacent motif (PAM). To expand target scope, it crucial to develop CRISPR toolbox capable of recognizing multiple PAMs. Here, using GFP-activation assay, we tested activities 29 type II-C orthologs closely related Nme1Cas9, 25 which are active in human cells. These recognize diverse PAMs with variable length and nucleotide preference, including purine-rich,...
Abstract Due to the tolerance of mismatches between gRNA and targeting sequence, base editors frequently induce unwanted Cas9-dependent off-target mutations. Here, develop models predict such off-targets, we design gRNA-off- target pairs for adenine (ABEs) cytosine (CBEs) stably integrate them into human cells. After five days editing, obtain valid efficiency datasets 54,663 55,727 off-targets ABEs CBEs, respectively. We use train deep learning models, resulting in ABEdeepoff CBEdeepoff,...
Broccoli (Brassica oleracea var. italica Plenck) is an important vegetable crop, as it rich in health-beneficial glucosinolates (GSLs). However, the genetic basis of GSL diversity Brassicaceae remains unclear. Here we report a chromosome-level genome assembly broccoli generated using PacBio HiFi reads and Hi-C technology. The final 613.79 Mb size, with contig N50 14.70 Mb. profile content analysis different B. varieties, combined phylogenetic tree analysis, sequence alignment, construction...
A bstract In this note we revisit the problem of explicitly computing tree-level scattering amplitudes in various theories any dimension from worldsheet formulas. The latter are known to produce cubic-tree expansion tree with kinematic numerators automatically satisfying Jacobi-identities, once half-integrand on is reduced logarithmic functions. We review a natural class functions called “Cayley functions”, which one-to-one correspondence labelled trees, and expansions half-integrands onto...
Abstract Clustered regularly interspaced short palindromic repeat (CRISPR)/SaCas9 is the most popular tool for in vivo genome editing due to its high efficiency and small genome. The authors previously developed four SaCas9 orthologs as genome‐editing tools. Here, expand targeting scope, they investigate diversity of protospacer adjacent motifs (PAMs) by screening a list 16 orthologs, twelve which display activity mammalian cells. They recognize five types PAMs: NNGRRT, NNGRRR, NNGRC, NNGA,...
Due to different nucleotide preferences at target sites, no single Cas9 is capable of editing all sequences. Thus, this highlights the need establish a repertoire covering sequences for efficient genome editing. Cas9s with simple protospacer adjacent motif (PAM) requirements are particularly attractive allow wide range editing, but identification such from thousands in public database challenge. We previously identified PAMs 16 SaCas9 orthologs. Here, we compared PAM-interacting (PI) domains...
Tree-level scattering amplitudes for gravitons, gluons, and Goldstone particles in any dimensions are strongly constrained by basic principles, they intimately related to each other via various relations. We study two types of ``universal expansions'' with respect gauge bosons bosons; the former expresses tree Einstein gravity (Yang-Mills) as linear combinations single-trace Einstein-Yang-Mills (Yang-Mills-${\ensuremath{\phi}}^{3}$) coefficients given Lorentz products polarizations momenta,...
A bstract All tree-level amplitudes in Einstein-Yang-Mills (EYM) theory and gravity (GR) can be expanded terms of color ordered Yang-Mills (YM) ones whose coefficients are polynomial functions Lorentz inner products constructed by a graphic rule. Once the gauge invariance condition any graviton is imposed, expansion tree level EYM or amplitude induces nontrivial identity between YM amplitudes. Being different from traditional Kleiss-Kuijf (KK) Bern-Carrasco-Johansson (BCJ) relations, induced...
Symmetries of Einstein-Yang-Mills (EYM) amplitudes, together with the recursive expansions, induce nontrivial identities for pure Yang-Mills amplitudes. In previous work [1], we have already proven that induced from tree level single-trace EYM amplitudes can be precisely expanded in terms BCJ relations. this paper, extend discussions to those all multi-trace Particularly, establish a refined graphic rule and then show fully decomposed
Abstract The CRISPR/Cas9 system derived from Streptococcus pyogenes (SpCas9) provides unprecedented genome editing capabilities, but the potential for off-target mutations limits its application. In addition to NGG protospacer adjacent motif (PAM), are also associated with noncanonical PAMs, which have not yet been systematically evaluated. Here, we developed a highly sensitive approach that allows analyzing PAM sequences in human cells, and identified multiple alternative PAMs recognized by SpCas9.
Many clustered regularly interspaced short palindromic repeat and CRISPR-associated protein 12b (CRISPR-Cas12b) nucleases have been computationally identified, yet their potential for genome editing remains largely unexplored. In this study, we conducted a GFP-activation assay screening 13 Cas12b mammalian editing, identifying five active candidates. Candidatus hydrogenedentes (ChCas12b) was found to recognize straightforward WTN (W=T or A) proto-spacer adjacent motif (PAM), thereby...
Abstract The compact CRISPR/Cas9 system, which can be delivered by adeno-associated virus (AAV), is a promising platform for therapeutic applications. However, current Cas9 nucleases have limited activity, targeting scope and specificity. Here, we identified three SaCas9 orthologs, Staphylococcus lugdunensis (SlugCas9), lutrae (SlutrCas9) haemolyticus (ShaCas9), mammalian genome editing. Interestingly, SlugCas9 recognizes simple NNGG PAM displays comparable activity to SaCas9. We further...
Abstract Autosomal dominant diseases can be treated by allele-specific disruption of mutant alleles. If the missense mutation generates a novel protospacer-adjacent motif (PAM), CRISPR-Cas9 distinguish alleles from wild-type PAM-specific approach. Therefore, it is crucial to develop CRISPR toolbox capable recognizing multiple PAMs. Here, using GFP-activation assay, we tested activities 29 type II-C orthologs closely related Nme1Cas9, 25 which are active in human cells. These recognize...
Received 20 November 2003DOI:https://doi.org/10.1103/PhysRevC.68.069901©2003 American Physical Society
Abstract Base editors, including adenine base editors (ABEs) and cytosine (CBEs), are valuable tools for introducing point mutations, but they frequently induce unwanted off-target mutations. Here, we performed a high-throughput gRNA-target library screening to measure editing efficiencies at integrated genomic off-targets obtained datasets of 48,632 52,429 ABE CBE, respectively. We used the train deep learning models, resulting in ABEdeepoff CBEdeepoff which can predict off-targets. These...