A5007055094- Immune Cell Function and Interaction
- Immune cells in cancer
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Oceanographic and Atmospheric Processes
- Genomics and Phylogenetic Studies
- Adenosine and Purinergic Signaling
- Ubiquitin and proteasome pathways
- Microbial metabolism and enzyme function
- Marine and coastal ecosystems
- Cancer-related Molecular Pathways
- Climate variability and models
- Immunotherapy and Immune Responses
- Atmospheric and Environmental Gas Dynamics
- Neonatal Respiratory Health Research
- Histone Deacetylase Inhibitors Research
- HIV Research and Treatment
- Photosynthetic Processes and Mechanisms
- Nanoplatforms for cancer theranostics
- Glycosylation and Glycoproteins Research
- Pluripotent Stem Cells Research
- Light effects on plants
- Galectins and Cancer Biology
- Cellular Mechanics and Interactions
Chongqing University
2025
Guangzhou First People's Hospital
2017-2025
Guangzhou Medical University
2017-2025
South China University of Technology
2025
Southern Medical University
2024
Second Affiliated Hospital of Xi'an Jiaotong University
2021-2024
Zhujiang Hospital
2024
Air Force General Hospital PLA
2024
Fujian Agriculture and Forestry University
2024
First Hospital of Jilin University
2023
Extensive genetic diversity in viral populations within infected hosts and the divergence of variants from existing reference genomes impede analysis deep sequencing data. A de novo population consensus assembly is valuable both as a single linear representation backbone on which intra-host can be accurately mapped. The availability assemblies robustly mapped are crucial to study disease progression, transmission dynamics, evolution. Existing techniques fail assemble ultra-deep sequence data...
Abstract Motivation: Error correction is critical to the success of next-generation sequencing applications, such as resequencing and de novo genome sequencing. It especially important for high-throughput short-read sequencing, where reads are much shorter more abundant, errors frequent than in traditional Sanger Processing massive numbers short with existing error methods both compute memory intensive, yet results far from satisfactory when applied real datasets. Results: We present a novel...
Human leukocyte antigen (HLA) typing at the allelic level can in theory be achieved using whole exome sequencing (exome-seq) data with no added cost but has been hindered by its computational challenge. We developed ATHLATES, a program that applies assembly, allele identification and pair inference to short read sequences, applied it from Illumina platforms. In 15 sets adequate coverage for HLA-A, -B, -C, -DRB1 -DQB1 genes, ATHLATES correctly reported 74 out of 75 pairs an overall...
Massively parallel sequencing offers the possibility of revolutionizing study viral populations by providing ultra deep (tens to hundreds thousand fold coverage) complete genomes. However, differentiation true low frequency variants from errors remains challenging.We developed a software package, V-Phaser 2, for inferring intrahost diversity within populations. This program adds three major new methodologies state art: technique efficiently utilize paired end read data calling phased...
RNA viruses are the causative agents for AIDS, influenza, SARS, and other serious health threats. Development of rapid broadly applicable methods complete viral genome sequencing is highly desirable to fully understand all aspects these infectious as well surveillance pandemic threats emerging pathogens. However, traditional detection rely on prior sequence or antigen knowledge. In this study, we describe sequence-independent amplification samples containing ultra-low amounts coupled with...
Macrophages exhibit phenotypic heterogeneity under both physiological and pathological conditions. Applications targeting M2-like tumor-associated macrophages (TAMs) improve outcome in solid tumors. Considerable differences are detected between leukemia-associated (LAMs) TAMs. However, application to induce M1 characteristics heterogeneous LAMs has not been established. Here we analyzed clinical relevance of macrophage phenotypes human acute myeloid leukemia (AML), studied evolution bone...
Next-generation sequencing (NGS) has the potential to transform discovery of viruses causing unexplained acute febrile illness (UAFI) because it does not depend on culturing pathogen or a priori knowledge pathogen's nucleic acid sequence. More generally, elucidate complete human virome, including that cause no overt symptoms disease, but may have unrecognized immunological developmental consequences. We used NGS identify RNA in blood 195 patients with UAFI and compared them those found 328...
ABSTRACT Human respiratory syncytial virus (RSV) is the leading cause of lower tract disease in infants and young children an important pathogen elderly immunocompromised. While population-wide molecular epidemiology studies have shown multiple cocirculating RSV genotypes revealed antigenic genetic change over successive seasons, little known about extent viral diversity course individual infection, origins novel variants, or effect immune pressure on potential immune-escape mutations. To...
Endothelial activation is a hallmark of the high-glucose (HG)-induced retinal inflammation associated with diabetic retinopathy (DR). However, precisely how HG induces endothelial not fully understood. We hypothesized that HG-induced up-regulation lysyl oxidase (LOX), collagen-cross-linking enzyme, in capillary cells (ECs) enhances subendothelial basement membrane (BM) stiffness, which, turn, promotes EC activation. Diabetic C57BL/6 mice exhibiting 70 and 50% increase intercellular adhesion...
Abstract Tumor-associated macrophages are widely studied in solid tumors. The distribution of lymph node samples was found to be associated with the prognosis lymphoma patients. However, role leukemia and their functional phenotypic characteristics hematopoietic malignancies have not been defined. In this study, we examined a Notch1-induced mouse model T cell acute lymphoblastic (T-ALL). bone marrow (BM) spleen, which proposed as BM spleen leukemia-associated (LAMs), were different during...
The introduction of West Nile virus (WNV) into North America in 1999 is a classic example viral emergence new environment, with its subsequent dispersion across the continent having major impact on local bird populations. Despite importance this epizootic, pattern, dynamics, and determinants WNV spread natural hosts remain uncertain. In particular, it unclear whether encountered barriers to transmission, or an unconstrained manner, if specific lineages were favored over others indicative...
Small RNAs (∼20 to 24 nucleotides) function as naturally occurring molecules critical in developmental pathways plants and animals [1], [2]. Here we analyze small RNA populations from mature rice grain seedlings by pyrosequencing. Using a clustering algorithm locate regions producing RNAs, classified hotspots of generation within the genome. Hotspots here are defined 1 kb which significantly overproduced relative rest were identified facilitate characterization different categories...
Abstract The ubiquitin–proteasome system (UPS) participates in both physiological and pathological processes through the posttranslational regulation of intracellular signal transduction pathways. F-box WD-40 domain protein 11 (Fbxw11) is a component SCF (Skp1–Cul1–F-box) E3 ubiquitin ligase complex. Fbxw11 regulates various pathways, it may have roles tumorigenesis. However, role development leukemia underlying mechanisms remain largely unknown. In this study, expression was aberrantly...
Mesoscale eddies are important to ocean circulation due their roles in the transport of mass, energy, and heat. This study employs a combination data sources initiate statistical analysis eddy spatiotemporal characteristics Bay Bengal (BOB) elucidate sea surface vertical structures impacts on chlorophyll (Chl) distributions. The results suggest that 1237 cyclonic (CEs) 1121 anticyclonic (AEs) were detected 26 years. number two polarities was almost same, most them spread west or southwest...
Messenger ribonucleic acid (mRNA) has emerged as a promising molecular preventive and therapeutic approach that opens new avenues for healthcare. Although the use of delivery systems, especially lipid nanoparticles (LNPs), greatly improves efficiency stability mRNA, mRNA tends to accumulate in liver hardly penetrates physiological barriers reach target site after intravenous injection. Hence, rational design targeting strategies aimed at directing specific tissues cells remains an enormous...
Background Cardiac fibroblasts (CFs) play a vital role in the physiological and pathological processes of heart. Previous studies have demonstrated that high glucose stimulation induces transformation CFs into myofibroblasts, contributing to cardiac fibrogenesis. However, vivo experiments predominantly utilized adult animals, whereas most vitro focused on derived from neonatal animals. The responses different age groups levels remain unclear. This study aimed investigate transcriptional...
The exponential growth of next generation sequencing (NGS) data has posed big challenges to storage, management and archive. Data compression is one the effective solutions, where reference-based strategies can typically achieve superior ratios compared ones not relying on any reference.This paper presents a lossless light-weight algorithm namely LW-FQZip compress FASTQ data. three components given input, i.e., metadata, short reads quality score strings, are first parsed into streams in...
Journal Article Matrix stiffness exerts biphasic control over monocyte–endothelial adhesion via Rho-mediated ICAM-1 clustering Get access Harry A. Scott, Scott Department of Bioengineering, University California Riverside, 900 Avenue, MSE 207, CA 92521, USA Search for other works by this author on: Oxford Academic Google Scholar Boi Quach, Quach Xiao Yang, Yang Soroush Ardekani, Ardekani Andrea P. Cabrera, Cabrera Randall Wilson, Wilson Division Biomedical Sciences, Ilhem Messaoudi-Powers,...
Nucleotides mediate intercellular communication by activating purinergic receptors and take part in various physiological pathological processes. Abnormal signaling plays important roles malignant progression. P2X7, which belongs to the P2X family of receptors, is abnormally expressed types malignancies including leukemia. However, its role molecular mechanism leukemia have not been elucidated. Here, we analyzed correlation between P2X7 expression AML clinical outcome; explored progression...
Macrophages play important roles in both physiologic and pathologic processes arise from successive waves of embryonic adult hematopoiesis. Monocyte-derived macrophages (MOMF) exert distinct functions under conditions, leukemia-associated (LAM) show considerable diversities activation functional phenotype. However, their origin have not been well elucidated. Here we used wild-type CCR2-/- mice to study the monocyte-derived LAM extramedullary tissues models Notch1-induced T-cell acute...