A5002087625- Entomopathogenic Microorganisms in Pest Control
- Plant-Microbe Interactions and Immunity
- Insect symbiosis and bacterial influences
- Plant Pathogens and Fungal Diseases
- Nematode management and characterization studies
- Insect Resistance and Genetics
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Metabolism and Genetic Disorders
- Insect and Arachnid Ecology and Behavior
- Mycorrhizal Fungi and Plant Interactions
- Mitochondrial Function and Pathology
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Protein Hydrolysis and Bioactive Peptides
- Transgenic Plants and Applications
- Ginseng Biological Effects and Applications
- Cardiovascular Issues in Pregnancy
- Fungal Biology and Applications
- Congenital Heart Disease Studies
- Mycotoxins in Agriculture and Food
- Enzyme-mediated dye degradation
- Microbial Metabolites in Food Biotechnology
- Coronary Artery Anomalies
- Vascular anomalies and interventions
Zhejiang Sci-Tech University
2020-2025
Zhejiang University
2014-2021
Institute of Microbiology
2017-2018
Guangdong General Hospital
2014-2015
Guangdong Academy of Medical Sciences
2014-2015
Significance Recently emerged fungal diseases are contributing toward global declines in wildlife, but the mechanisms for emergence of new pathogenic fungi remain mysterious. The entomopathogen Metarhizium robertsii evolved from plant symbionts, and here we report that this host shift was facilitated by 18 horizontal gene transfers (HGTs). necessity breaching cuticular barriers selected retention these genes, as 12 up-regulated during penetration, their collective actions indispensable...
Summary M etarhizium robertsii has been used as a model to study fungal pathogenesis in insects, and its pathogenicity many parallels with plant mammal pathogenic fungi. MAPK (Mitogen‐activated protein kinase) cascades play pivotal roles cellular regulation fungi, but their functions have not characterized . In this study, we identified the full complement of cascade components dissected regulatory pathogenesis, conidiation stress tolerance. The nine Fus3, Hog1 Slt2‐ are all involved...
Systematic gene disruption is a direct way to interrogate fungal genome functionally characterize the full suite of genes involved in various biological processes. Metarhizium robertsii extraordinarily versatile, and it pathogen arthropods, saprophyte beneficial colonizer rhizospheres. Thus, M. can be used as representative simultaneously study several major lifestyles that are not shared by "model" fungi Saccharomyces cerevisiae Neurospora crassa; systematic genetic analysis will benefit...
Histone deacetylation normally decreases the gene expression in organisms. By genome-wide deletions of epigenetic regulators entomopathogenic fungus Metarhizium robertsii, unexpected activations orphan secondary metabolite genes have been found upon disruption a histone acetyltransferase (HAT) Hat1. This led to characterization 11 new natural products, including eight isocoumarin derivatives meromusides A-H and two nonribosomal peptides meromutides A B. Therefore, HAT represents approach...
Abstract Metarhizium robertsii is a versatile fungus with saprophytic, plant symbiotic and insect pathogenic lifestyle options. Here we show that M. mediates the saprophyte-to-insect pathogen transition through modulation of expression membrane protein, Mr-OPY2. Abundant Mr-OPY2 protein initiates appressorium formation, prerequisite for infection, whereas reduced production elicits saprophytic growth conidiation. The precise regulation achieved via alternative transcription start sites....
The ecological importance of the duplication and diversification gene clusters that synthesize secondary metabolites in fungi remains poorly understood. Here, we demonstrated subsequent a cluster produced two polyketide synthase cosmopolitan fungal genus Metarhizium. Diversification occurred promoter regions exon-intron structures Pks paralogs (Pks1 Pks2). These genes have distinct expression patterns, with Pks1 highly expressed during conidiation Pks2 infection. Different upstream signaling...
As a type of multicopper oxidase, laccases play multiple biological roles in entomopathogenic fungi, enhancing their survival, development, and pathogenicity. However, the mechanisms by which operate these fungi remain under-researched. In this study, we identified two laccase-encoding genes, Mr-lac3 Mr-lcc2, from Metarhizium robertsii, both are highly expressed during conidiation. Knocking out Mr-lcc2 resulted significant increase conidial yields M. robertsii. Furthermore, relative...
Coix seed is a valuable crop with both medicinal and edible uses, significantly impacting economic medical sectors. However, its contamination fungi mycotoxins poses serious health risks when used medicinally. Thus, identifying in controlling fungal toxins crucial. This study employs high-throughput sequencing to detect samples analyze their correlation mycotoxin content. Our findings showed no infection from filling maturity, but diverse community was present mature seeds, the relative...
The aim of the present study was to explore toxic effects and underlying mechanisms nickel ions during therapeutic nickel‑based alloy‑treatment in congenital heart disease by investigating metal‑induced cytotoxicity human monocyte‑derived macrophage cell line THP‑1. THP‑1 cells were treated with NiCl2·6H2O (25, 50, 100, 200, 400 800 µM) for 24, 48 72 h, respectively. MTT applied detect proliferation following NiCl2 treatment. Apoptosis quantified using flow cytometry. Illumina sequencing...
Summary Metarhizium robertsii occupies a wide array of ecological niches and has diverse lifestyle options (saprophyte, insect pathogen plant symbiont), that renders it an unusually effective model for studying genetic mechanisms fungal adaptation. Here over 20,000 M. T‐DNA mutants were screened in order to elucidate mechanism by which replicates persists niches. About 287 conidiation, colony sectorization or pathogenicity loci, many have not been reported other fungi identified. By...
Nigrospora nonsegmented RNA virus 1 (NoNRV1) has been reported previously in the fungus oryzae, but its biological effects on host are unknown. In this work, we isolated a strain 9-1 of N. oryzae from chrysanthemum leaf and identified NoNRV1 infection strain. The genome sequence here is highly homologous to that isolate HN-21 reported; thus, tentatively designated newly as NoNRV1-ZJ. Drug treatment with Ribavirin successfully removed NoNRV1-ZJ 9-1, which provided us an ideal control...
Siderophores are small molecular iron chelators and participate in the multiple cellular processes fungi. In this study, biosynthesis gene clusters of coprogens dimerumic acids were identified by transcriptional level differences genes related to deficiency conditions Metarhizium robertsii. This leads characterization new coprogen metachelin C (1) five known siderophores A (2), A-CE (3), B (4), acid 11-mannoside (5), (6). The structure was elucidated NMR spectroscopy HR-ESI-MS analysis....
Fungal pathogens widely infect plants and fruits, causing various diseases reducing their yield quality. Biological control provides a good strategy for improving crop productivity quality as well the use of potentially environmentally hazardous chemical fertilizers pesticides. In this study, antagonistic functional components strain Bacillus velezensis 1-10, isolated from acidic soil tea gardens were investigated. B. 1-10 inhibited growth four fungal based on plate confrontation tests...
Aspergillus flavus is a saprophytic soil fungus that infects and contaminates seed crops with the highly carcinogenic aflatoxin, which brings health hazards to animals humans. In this study, bacterial strains B1 B2 isolated from rhizosphere of camellia sinensis had significant antagonistic activities against A. flavus. Based on phylogenetic analysis 16SrDNA gene sequence, were identified as Bacillus tequilensis velezensis, respectively. addition, transcriptome showed some genes related...
Saponins are kinds of antifungal compounds produced by Panax notoginseng to resist invasion pathogens. Ilyonectria mors-panacis G3B was the dominant pathogen inducing root rot P. notoginseng, and abilities detoxify saponins were key infect successfully. To research molecular mechanisms detoxifying in I. G3B, we used high-throughput RNA-Seq identify 557 1519 differential expression genes (DEGs) with treatments for 4H (Hours) 12H compared no treatments, respectively. Among these DEGs, found 93...
Objective To evaluate the efficacy and experience in right ventricular pacing-percutaneous balloon aortic valvuloplasty (RVP-PBAV) for congenital stenosis (AS). Method A total of sixteen children with AS accepted treatment RRVP-PBAV. The patients were at ages 6 months to 15 years, their median age was 5.4 years. Their body weight between 8.5 59.0 kg, average (22.3±16.5) kg. gradient pressure across valve measured all regurgitation observed. follow-up time ranged from 1 month 5.5...
The present study aimed to specifically transform stevioside (ST) into rubusoside (RS) through bioconversion with high efficiency, seeking endow steviol glycosides (SGs) subtle flavours for commercial acceptability.An endophytic bacterium named Lelliottia LST-1 was screened and confirmed convert ST RS, reaching a conversion rate of 75.4% after response surface optimization. Phylogenetic analysis combined complete genome sequencing demonstrated that also presumed be new species. To further...