A5102990884- Physiological and biochemical adaptations
- Spaceflight effects on biology
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Reproductive Health and Technologies
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Arctic and Antarctic ice dynamics
- Extracellular vesicles in disease
- Colorectal Cancer Treatments and Studies
- High Altitude and Hypoxia
- DNA Repair Mechanisms
- Ferroptosis and cancer prognosis
- Trauma and Emergency Care Studies
- Genomics and Chromatin Dynamics
- Drug Transport and Resistance Mechanisms
- Microtubule and mitosis dynamics
- Traffic and Road Safety
- RNA Research and Splicing
- FOXO transcription factor regulation
- Cancer Research and Treatments
- Hedgehog Signaling Pathway Studies
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
Princess Margaret Cancer Centre
2018-2021
University Health Network
2016-2021
Hong Kong Baptist University
2017-2020
University of Toronto
2010-2013
This paper is the first report of robotic intracytoplasmic sperm injection (ICSI). ICSI a clinical procedure performed worldwide in fertility clinics, requiring pick-up single and insertion it into an oocyte (i.e., egg cell). Since its invention 20 years ago, has been conducted manually by handful highly skilled embryologists; however, success rates vary significantly among clinics due to poor reproducibility inconsistency across operators. We leverage our work cell realize aim ultimately,...
Abstract In embryonic stem cells, promoters of key lineage-specific differentiation genes are found in a bivalent state, having both activating H3K4me3 and repressive H3K27me3 histone marks, making them poised for transcription upon loss H3K27me3. Whether cancer-initiating cells (C-ICs) have similar epigenetic mechanisms that prevent lineage commitment is unknown. Here we show colorectal C-ICs (CC-ICs) maintained stem-like state through mechanism. Disruption the inhibition H3K27...
Abstract Purpose: Cancer-initiating cells (C-IC) have been described in multiple cancer types, including colorectal cancer. C-ICs are defined by their capacity to self-renew, thereby driving tumor growth. were initially thought be static entities; however, recent studies determined these dynamic and influenced microenvironmental cues such as hypoxia. If hypoxia drives the formation of C-ICs, then therapeutic targeting could represent a novel means target C-ICs. Experimental Design:...
Human stanniocalcin-1 (STC1) is a paracrine factor associated with inflammation and carcinogenesis. The role of STC1 in the pro- anti-inflammatory functions differentiating macrophage, however, not clear. In this study, our data showed that phorbol 12-myristate 13-acetate (PMA) treatment induced human leukemia monocytic cells (ThP-1) differentiation to M0 macrophages. was accompanied by significant increase mRNA expression levels STC1, pro-inflammatory cytokine TNFα, markers, CD163 & CD206....
Stanniocalcin-1 (STC1) is a paracrine factor associated with inflammation and carcinogenesis. Using clinicopathological data, we previously reported that greater expression of STC1 in hepatocellular carcinoma (HCC) was significantly correlated smaller tumor size. The underlying mechanism on the correlation not known. In this study, using metastatic HCC cell-line (MHCC-97L, P) lentiviral vector mediated-STC1 overexpression, inoculation STC1-overexpressing MHCC-97L (S1) cells nude mice...
Human stanniocalcin-1 (STC1) is a glycoprotein known to participate in inflammation and tumor progression. However, its role cancer-macrophage interaction at the environment not known. In this study, co-culture of human metastatic hepatocellular carcinoma cell line (MHCC97L) stably transfected with control vector (MHCC97L/P), or STC1-overexpressing (MHCC97L/S1) leukemia monocytic (THP-1) was conducted. We reported that MHCC97L/S1 suppressed migratory activity THP-1. Real-time PCR analysis...
Abstract Despite advances in treatment, Colorectal cancer (CRC) remains a leading cause of related deaths North America. Most CRCs contain subset self-renewing colon cancer-initiating cells (CC-ICs) responsible for tumor initiation and maintenance. CC-ICs are relatively chemoresistant as compared to the bulk cells, however mechanisms this chemoresistance poorly understood. An increasing amount evidence suggests that epigenetic regulators may be playing central role driving CC-IC...
Abstract Human stanniocalcin-1 (STC1) is a paracrine factor associated with inflammation and carcinogenesis. The role of STC1 in the pro- anti-inflammatory functions differentiating macrophage, however, not clear. In this study, our data showed that PMA treatment induced human leukemia monocytic cells (ThP-1) differentiation to M0 macrophages. was accompanied by significant increase mRNA expression levels STC1, pro-inflammatory cytokine TNFα, markers, CD163 & CD206. An intermitted...