A5022136580- Abdominal Surgery and Complications
- Microbial Natural Products and Biosynthesis
- Antifungal resistance and susceptibility
- Parasitic infections in humans and animals
- Cardiac, Anesthesia and Surgical Outcomes
- Porphyrin and Phthalocyanine Chemistry
- Plant-Microbe Interactions and Immunity
- Microbial Metabolism and Applications
- Congenital Anomalies and Fetal Surgery
- Marine Sponges and Natural Products
- Enzyme Production and Characterization
- Plant Pathogens and Fungal Diseases
- Enzyme Catalysis and Immobilization
- Colorectal Cancer Surgical Treatments
- Abdominal Trauma and Injuries
- Muscle and Compartmental Disorders
- Bacterial biofilms and quorum sensing
- Global Health and Surgery
- Multiple and Secondary Primary Cancers
- Parasitic Diseases Research and Treatment
- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- Glycosylation and Glycoproteins Research
- Escherichia coli research studies
- Essential Oils and Antimicrobial Activity
University of Belgrade
2012-2024
Military Medical Academy
2012
Institute of Molecular Genetics and Genetic Engineering
2005-2009
Sportska Akademija Beograd
1997
Bacterial infections have become increasingly difficult to treat due the occurrence of antibiotic-resistant strains. A promising strategy increase efficacy therapy is combine antibacterials with agents that decrease pathogen virulence via modulation quorum sensing (QS). Lactonases inhibit acylated homoserine lactone (AHL)-mediated QS in Gram-negative bacteria, including leading nosocomial Pseudomonas aeruginosa . Here we describe characteristics heterologously expressed YtnP lactonase from...
Abstract The adaptability of halophytes to increased soil salinity is related complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used analyze the bacterial communities in endorizosphere indigenous succulent Salicornia europaea , Suaeda maritima Camphorosma annua from natural salt marshes Slano Kopovo (Serbia). 16 S rDNA analyses gave, for first time, insight into composition endophytic S. C. . We have...
The aim of this study was to improve production pentaene 32,33‐didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain obtain quantities sufficient for depth analysis antimicrobial properties. Through classical medium optimization conditions stable growth, DDHR within 7 days incubation established. Yields 215 mg l−1 were achieved shake flask experiments complex with mannitol as the primary carbon source. had poor antibacterial activity minimal inhibitory concentrations (MIC)...
Abstract A complete, integrated process for the production of an innovative formulation penicillin G acylase from Providencia rettgeri (rPAC P.rett )of industrial applicability is reported. In order to improve yield rPAC, clone LN5.5, carrying four copies pac gene into genome Pichia pastoris , was constructed. The proteinase activity recombinant strain reduced by knockout PEP4 encoding A, resulting in increased rPAC approximately 40% (3.8 U/mL vs. 2.7U/mL produced LN5.5 flask). high cell...
The aim of this study was to address the toxicity recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range fungal pathogens and its potential control plant diseases.The antifungal activity DDHR in vitro examined against common human pathogenic fungi using broth microdilution assay disk diffusion assay. Minimum inhibitory concentrations ranged from 12·5 35 μg ml(-1) . A radial growth inhibition showed that inhibited mycelia growth, inducing mycelial necrosis...
Penicillin G acylase (PAC) is one of the most widely used enzymes in industrial synthesis semi-synthetic antibiotics. The Providencia rettgeri pac gene was expressed to a level 2.7 U/ml using Pichia pastoris expression system. recombinant enzyme purified and its glycosylation status determined. It found that both subunits (? ?) were N-glycosylated, while ?-subunit also contained O-glycans. observed rPACP.rett. stable wide range pH, which, addition previously proved high thermostability,...
Herbicides are integral to agricultural weed management but can adversely affect non-target organisms, soil health, and microbiome. We investigated the effects of herbicides on total bacterial community composition using 16S rRNA gene amplicon profiling. Further, we aimed identify herbicide-tolerant bacteria with plant growth-promoting (PGP) capabilities as a mitigative strategy for these negative effects, thereby promoting sustainable practices. A analysis explored long-term S-metolachlor...
In exploring a growing demand for innovative approaches to tackle emerging and life threatening fungal diseases, we identified long-chain 4-aminoquinoline (4-AQ) derivatives as new class of anti-virulence agents. For the first time, demonstrated that 4-AQs effectively prevent filamentation Candida albicans, key virulence trait, under multiple triggering conditions. Selected 4-AQ inhibited filament formation in zebrafish model disseminated candidiasis at 1.56 µM, with no toxicity up 50 µM....
Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into biosynthesis these gained after genome sequencing and identification genes involved. Analysis draft revealed that it is closely related several strains S. griseus. Comparative analyses secondary metabolite biosynthetic gene clusters, as well those presumably involved in acid biosynthesis, allowed an unusual cluster C12-2, which could be identified...
Candidiasis caused by Candida albicans is one of the most common fungal infections in modern society. The limited arsenal clinical drugs, their side effects and emerging resistance, largely contribute to low efficacy current antifungal therapies. morphogenetic yeast-to-hyphae transition key virulence feature for establishment local systemic C. infections. We show that a delfinidin-rich fruit extract from Aristotelia chilensis ([Molina], Stuntz) (maqui berry) inhibits filamentation albicans,...
In this paper we describe the effect of kanamycin resistance gene (Kmr) on 2?m-based plasmid maintenance in Saccharomyces cerevisiae. The influence marker loss stable model-vectors proved to be constant, as well independent carbon source and culture growth rates. strains for GALUAS - driven heterologous protein production introduction Kmr resulted curing yeast episomal (YEp) from population a small number generations. Application selective pressure strain producing recombinant penicillin G...