A5046821470- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Pineapple and bromelain studies
- Protein Hydrolysis and Bioactive Peptides
- Nanocomposite Films for Food Packaging
- Biochemical and Structural Characterization
- Electrochemical sensors and biosensors
- Seaweed-derived Bioactive Compounds
- Carbohydrate Chemistry and Synthesis
- Phytase and its Applications
- Advanced Drug Delivery Systems
- Transgenic Plants and Applications
- Food composition and properties
- Bacterial biofilms and quorum sensing
- Microbial Metabolic Engineering and Bioproduction
- Click Chemistry and Applications
- Mesoporous Materials and Catalysis
- Proteins in Food Systems
- Protein Interaction Studies and Fluorescence Analysis
- biodegradable polymer synthesis and properties
- Algal biology and biofuel production
- Synthesis and biological activity
- Papaya Research and Applications
- Polysaccharides Composition and Applications
Voronezh State University
2016-2025
Marine Hydrophysical Institute
2020-2025
Sevastopol National Technical University
2022-2024
Voronezh State Medical Academy named after N.N. Burdenko
2022
Abstract Biofilms, the communities of surface-attached bacteria embedded into extracellular matrix, are ubiquitous microbial consortia securing effective resistance constituent cells to environmental impacts and host immune responses. Biofilm-embedded generally inaccessible for antimicrobials, therefore disruption biofilm matrix is potent approach eradicate biofilms. We demonstrate here destruction Staphylococcus aureus epidermidis biofilms with Ficin, a nonspecific plant protease. The...
Chitosan, the product of chitin deacetylation, is an excellent candidate for enzyme immobilization purposes. Here we demonstrate that papain, endolytic cysteine protease (EC: 3.4.22.2) from Carica papaya latex immobilized on matrixes medium molecular (200 kDa) and high (350 weight chitosans exhibits anti-biofilm activity increases antimicrobials efficiency against biofilm-embedded bacteria. Immobilization in glycine buffer (pH 9.0) allowed adsorption up to 30% total protein (mg g chitosan−1)...
This study explores various methods for the covalent immobilization of cysteine proteases (ficin, papain, and bromelain). Covalent involves formation bonds between enzyme a carrier or molecules themselves without using crosslinking agent. process enhances stability allows creation preparations with specific controlled properties. The objective this is to evaluate impact under different conditions on proteolytic activity enzymes. most favorable results were achieved by immobilizing ficin...
The viability and surface morphology of human erythrocytes upon interaction with oligochitosan (OCH), having a molecular weight (MW) 6.2-15.4 kDa degree acetylation (DA) 1-2%, N-reacetylated OCH (ROCH) 6.4-14.3 MW 24-30% DA were studied in isotonic saline phosphate buffer pH 7.4. It was shown that the use caused high hemolysis irreversible transformation erythrocytes. Thus, 6.2 1% DA, used at 0.01% concentration, induced erythrocytes, their did not exceed maximal value 60%. Among...
This work aims to synthesize graft copolymers of chitosan and N-vinylimidazole (VI) with different compositions be used as matrices for the immobilization cysteine proteases-bromelain, ficin, papain. The are synthesized by free radical solution copolymerization a potassium persulfate-sodium metabisulfite blend initiator. have relatively high frequency grafting yields. All water-soluble, their solutions characterized DLS laser Doppler microelectrophoresis. self-assembled in aqueous solutions,...
This study investigates the features of interactions between cysteine proteases (bromelain, ficin, and papain) a graft copolymer carboxymethyl cellulose sodium salt with N-vinylimidazole. The objective is to understand influence this on proteolytic activity stability enzymes. enzymes were immobilized through complexation carrier. interaction mechanism was examined using Fourier-transform infrared spectroscopy flexible molecular docking simulations. findings reveal that interact functional...
Briefly, 2-(4-Acetamido-2-sulfanilamide) chitosan, which is a chitosan water-soluble derivative, with molecular weights of 200, 350, and 600 kDa, was successfully synthesized. The immobilization ficin, papain, bromelain carried out by complexation these polymers. interaction mechanism 2-(4-acetamido-2-sulfanilamide) bromelain, papain studied using FTIR spectroscopy. It found that the hydroxy, thionyl, amino groups were involved in process. Molecular docking research showed all acid residues...
Micro- and nanoparticles of chitosan carboxymethyl were synthesized, both with without ascorbic acid. Methods developed to form complexes between these micro- plant proteases-ficin, papain, bromelain. It was demonstrated that the activity cysteine protease higher compared those nanoparticles. Additionally, ficin, bromelain synthesized in presence acid exhibited greater proteolytic than formed particles prepared Molecular docking studies revealed amino residues primarily interact through...
Enzyme immobilization on various carriers represents an effective approach to improve their stability, reusability, and even change catalytic properties. Here, we show the mechanism of interaction cysteine protease bromelain with water-soluble derivatives chitosan-carboxymethylchitosan,
The present work is devoted to research on the interaction between carboxymethyl cellulose sodium salt and its derivatives (graft copolymer of N,N-dimethyl aminoethyl methacrylate) with cysteine protease (ficin). was studied by FTIR flexible molecular docking, which have shown conjugates' formation both matrices. proteolytic activity assay performed azocasein demonstrated that specific activities all immobilized ficin samples are higher in comparison those native enzyme. This due modulation...
In the last decade, Ficin, a proteolytic enzyme extracted from latex sap of wild fig tree, has been widely investigated as promising tool for treatment microbial biofilms, wound healing, and oral care. Here we report antibiofilm properties immobilized on soluble carboxymethyl chitosan (CMCh) CMCh itself. Ficin was with molecular weights either 200, 350 or 600 kDa. Among them, carrier weight 200 kDa bound maximum amount enzyme, binding up to 49% total protein compared 19-32% other CMChs....