A5047002371- Xenotransplantation and immune response
- Animal Genetics and Reproduction
- Organ Transplantation Techniques and Outcomes
- Corneal surgery and disorders
- Pancreatic function and diabetes
- Corneal Surgery and Treatments
- Virus-based gene therapy research
- Transplantation: Methods and Outcomes
- Pancreatic and Hepatic Oncology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic factors in colorectal cancer
- Tissue Engineering and Regenerative Medicine
- Neuroendocrine Tumor Research Advances
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Lung Cancer Research Studies
- Healthcare cost, quality, practices
- Colorectal Cancer Screening and Detection
- Cannabis and Cannabinoid Research
- Ovarian cancer diagnosis and treatment
- Vascular Tumors and Angiosarcomas
- Sperm and Testicular Function
- Gastrointestinal Tumor Research and Treatment
- Cardiac Ischemia and Reperfusion
- Tuberous Sclerosis Complex Research
Medical College of Wisconsin
2024
University of California, Irvine
2020-2023
University of California, Irvine Medical Center
2021-2023
University of Pittsburgh
2013-2020
University of Pittsburgh Medical Center
2015
Our group previously investigated the levels of anti-Gal and anti-nonGal IgM IgG in a cohort 75 healthy humans various backgrounds, found some significant differences related to factors such as age, gender, ABO blood group, diet, vaccination history, geographic location during childhood. We have now expanded our (n = 84) investigate anti-Neu5Gc anti-nonGal/nonNeu5Gc antibodies humans. Anti-nonGal human binding pRBCs pAECs from GTKO/CD46 GTKO/CD46/Neu5GcKO pigs were measured by flow...
Absract Background The impact that the absence of expression NeuGc in pigs might have on pig organ or cell transplantation humans has been studied vitro , but only using red blood cells (p RBC s) and peripheral mononuclear PBMC as target for immune assays. We extended this work various models now report our initial results. Methods we used involve GTKO /hCD46 /hCD46/NeuGcKO aortas corneas, p s, aortic endothelial AEC s), corneal CEC isolated pancreatic islets. investigated effect (i) human...
To investigate whether decellularization using different techniques can reduce immunogenicity of the cornea, and to explore decellularized cornea as a scaffold for cultured corneal endothelial cells (CECs). Transplantation porcine corneas increases graft transparency survival longer periods compared with fresh grafts.Six-month-old wild-type pig were cut into 100-200 µm thickness, then by three methods: 1) 0.1% sodium dodecyl sulfate (SDS); 2) hypoxic nitrogen (N2); 3) hypertonic NaCl....
Abstract Background Old World non‐human primates (OWNHPs) are used for preclinical pig‐to‐NHP studies. However, like pigs, OWNHPs express Neu5Gc, and therefore do not develop natural anti‐Neu5Gc antibodies. New NHPs (NWNHPs) have been reported to Neu5Gc. We investigated the potential of NWNHPs in xenotransplantation research. Methods expression Gal, Sd a antigens on RBCs PBMCs from humans, selected OWNHPs, capuchin monkeys (a NWNHP). Serum anti‐Gal IgM IgG levels were measured by ELISA....
Abstract Background Glutaraldehyde‐fixed bioprosthetic heart valves ( GBHV s) derived from wild‐type WT , genetically unmodified) pigs are widely used clinically for valve replacement. There is evidence that their failure related to an immune response. The use of engineered do not express specific pig antigens may prolong survival. Our aims were determine (i) expression Gal and NeuGc on (aortic pulmonary) pericardium α1,3‐galactosyltransferase gene knockout GTKO ) / N ‐glycolylneuraminic...
The aim of this study was to investigate the distribution antigens other than galactose-α-1,3-galactose (Gal) (non-Gal) recognized by human and rhesus monkey serum antibodies in α-1,3-galactosyltransferase gene-knockout (GTKO) pig cornea.The non-Gal, specifically N-glycolylneuraminic acid (NeuGc), corneas from wild-type (WT) GTKO pigs identified. Corneal sections WT were incubated with or determine immunoglobulin (Ig)M IgG binding corneal tissue means fluorescent microscopy.Strong expression...
In Brief Background Serum (extracellular) histone levels are increased in inflammatory states and the presence of coagulation dysfunction, for example, trauma, chemical/ischemic injury, infection. There is increasing evidence a systemic response associated with pig xenograft nonhuman primate. We evaluated extracellular baboons various xenografts. Methods measured serum histones heterotopic heart (n = 8), life-supporting kidney 5), orthotopic liver 4), artery patch 9) grafts by enzyme-linked...
Purpose: Endothelial keratoplasty (EKP) has become increasingly popular in the treatment of corneal disease. However, global shortage human donor corneas limits clinical transplantation. Genetically engineered (GE) pigs may provide an alternative source for EKP. The aim this study was to evaluate endothelial cells (CECs) from wild-type (WT) and GE pigs. Methods: Density, size CECs, percentage hexagonal (as a measure heterogeneity) were measured by ex vivo confocal microscopy WT different...
Purpose: Pigs expressing neither galactose-α1,3-galactose (Gal) nor N-glycolylneuraminic acid (NeuGc) take xenotransplantation one step closer to the clinic. Our aims were (1) document lack of NeuGc expression on corneas and aortas cultured endothelial cells [aortic (AECs); corneal (CECs)] GTKO/NeuGcKO pigs, (2) investigate whether absence reduced human antibody binding tissues cells. Methods: Wild-type (WT), GTKO, pigs used for study. Human negative controls. Immunofluorescence staining was...
To investigate the effect of Rho-kinase inhibitor, Y27632, on pig corneal endothelial cell (pCEC) culture, and inflammation immune regulation responses human cells to pCECs.pCECs were cultured with/without Y27632 assess proliferation in vitro wound healing assay. The level MCP-1 VEGF pCECs stimulated with TNF-α measured. Proliferation PBMCs pCECs, cytokine production T cells, monocyte migration after stimulation investigated.Y27632 promoted pCEC proliferation, prevented death, enhanced...
Ischemia-reperfusion injury (IRI) is an intrinsic risk associated with liver transplantation. Ex vivo hepatic machine perfusion (MP) emerging organ preservation technique that can mitigate IRI, especially in livers subjected to prolonged warm ischemia time (WIT). However, a method quantify the biological response WIT during MP has not been established. Previous studies used physiologically based pharmacokinetic (PBPK) modeling demonstrate decrease transport and biliary excretion of tracer...
Abstract Background Some patients with acute or acute‐on‐chronic hepatic failure die before a suitable human liver allograft becomes available. Encouraging results have been achieved in such by the transplantation of hepatocyte progenitor cells from fetal tissue. The aim study was to explore survival hepatocytes genetically engineered pigs after direct injection into spleen and other selected sites immunosuppressed baboons monitor immune response metabolic function transplanted hepatocytes....
Abstract Recent reports of long‐term survival after wild‐type ( WT ) pig‐to‐monkey corneal xenotransplantation are encouraging. We experienced the rapid development retrocorneal membranes, a rare complication allotransplantation (although seen in infants and young children). The original specific aim study was to determine factors associated with successful (young) pig transplantation monkeys. However, when it obvious that membranes rapidly developed, our aims became involved its both...
Transplantation of islets into the gastric submucosal space (GSMS) has several advantages (e.g., avoidance instant blood-mediated inflammatory response [IBMIR], ability to biopsy). The aim this study was determine whether endoscopic biopsy islet allografts transplanted GSMS in diabetic pigs can provide histopathological and immunohistochemical information that correlates with clinical course (e.g.,, blood glucose level, insulin requirement). Islet (Group1: 10,000 kIEq /kg [n = 4]; Group2:...
Article Tools CASE REPORTS OPTIONS & TOOLS Export Citation Track Add To Favorites Rights Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/PO.22.00230 JCO Precision Oncology no. 7 (2023) e2200230. Published online January 9, 2023. PMID: 36623237 Rapid and Deep Response to Lorlatinib in Pancreatic High-Grade Neuroendocrine Carcinoma With a Treatment Emergent Novel KANK1-ALK Fusion Farshid Dayyani , MD, PhD1xFarshid DayyaniSearch for by this author; Whayoung...