A5056392683- Pregnancy and preeclampsia studies
- Drug Transport and Resistance Mechanisms
- Pregnancy and Medication Impact
- Reproductive System and Pregnancy
- Pharmacological Effects and Toxicity Studies
- Birth, Development, and Health
- Adenosine and Purinergic Signaling
- Amino Acid Enzymes and Metabolism
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- Estrogen and related hormone effects
- Prenatal Substance Exposure Effects
- Ion Transport and Channel Regulation
- Neonatal Respiratory Health Research
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
- Click Chemistry and Applications
- Reproductive Health and Contraception
- Gout, Hyperuricemia, Uric Acid
- Barrier Structure and Function Studies
- Preterm Birth and Chorioamnionitis
- Connexins and lens biology
- Single-cell and spatial transcriptomics
- Pharmaceutical studies and practices
- Heart Failure Treatment and Management
Keio University
2015-2024
Yokohama University of Pharmacy
2020-2021
Daiichi Sankyo (United Kingdom)
2019
We evaluated the contribution of organic anion transporting polypeptide 2A1 (OATP2A1/SLCO2A1), a high-affinity carrier for prostaglandins (PGs), to parturition process. At gestational day (GD) 15.5, OATP2A1 is co-localized with 15-hydroxy-PG dehydrogenase in mouse placental junctional zone and facilitates PG degradation by delivering PGs cytoplasm. Slco2a1 (+/-) females mated (-/-) males frequently showed elevated circulating progesterone at GD18.5 delayed parturition. Progesterone receptor...
The aims of this study are to quantify the protein levels nucleoside transporters in placental microvillous membranes (MVMs) and clarify contributions these ribavirin uptake at barrier. Placental MVMs human rat expressed equilibrative transporter (ENT) 1 protein, whereas expression ENT2 was obscure. Maternal-to-fetal transfer [3H]ribavirin rats much higher than that [14C]sucrose. by trophoblast TR-TBT 18 d-1 cells, which functionally express both ENT1 proteins, saturable, significantly...
Two types of systems are used in ex vivo human placental perfusion studies to predict fetal drug exposures, that is, closed with recirculation the maternal and buffer open using a single-pass mode without recirculation. The fetal/maternal (F:M) ratio metformin, cationic crosses placenta, is consistent reported an system but not system. In present study, we aimed develop pharmacokinetic (PK) model transplacental transfer metformin exposure resolve apparent inconsistency between systems....
Multidrug resistance protein 1 (MDR1) is located at the interface between two syncytiotrophoblast layers in rodent placenta, and may influence fetal drug distribution. Here, we quantitatively compare functional impact per single MDR1 molecule of placental barrier blood-brain mice.MDR1A MDR1B proteins were quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Paclitaxel or digoxin was continuously administered to pregnant Mdr1a-/-/Mdr1b-/- wild-type mice, concentrations...
Tadalafil, a phosphodiesterase 5 (PDE5) inhibitor, is candidate therapeutic agent for fetal growth restriction and hypertensive disorders of pregnancy. In this study, we elucidated the transfer tadalafil in comparison with that sildenafil, first PDE5 inhibitor to be approved. We also examined contributions multidrug resistance protein 1 (MDR1) breast cancer (BCRP) transfer. Tadalafil or sildenafil was administered wild-type, Mdr1a/b-double-knockout Bcrp-knockout pregnant mice by continuous...
Background: Milademetan (DS-3032b) is a small-molecule MDM2 inhibitor that activates p53 and induces apoptosis by disrupting the MDM2-p53 interaction. has demonstrated single-agent antileukemic activity in preclinical clinical studies AML. AZA, hypomethylating agent, used to treat AML myelodysplastic syndromes upregulating epigenetically silenced genes cells. We conducted study of milademetan combination with AZA because both agents modulate gene expression related cell growth or apoptosis....
Equilibrative nucleoside transporters (ENTs) 1 and 2 reportedly accept fluorouracil as a substrate. Here, we evaluated ENT1/2 expression at the messenger RNA (mRNA), protein, functional levels in panel of four triple-negative breast cancer (TNBC) cell lines, BT-549, Hs578T, MDA-MB-231, MDA-MB-435, examined relationship observed profiles to sensitivity. Nitrobenzylthioinosine (NBMPR) 0.1 μM inhibits only ENT1, while dipyridamole 10 or NBMPR 100 both ENT1 ENT2. We found that uptake [3...
This study aimed to investigate the meaning of sound environment for children with special needs. We conducted action research on room acoustics in a child development support center. First, we investigated actual conditions through acoustic measurement, behavioral observation, and interviews teachers. Results revealed loud voices, example, crying screaming as daily sounds. The teachers had awareness problems environment. In addition, it was observed that teacher personally engaged their...
Digoxin is used as first-line therapy to treat fetal supraventricular tachycardia; however, because of the narrow therapeutic window, it essential estimate digoxin exposure in fetus. The data from ex vivo human placental perfusion study are predict noninvasively, but fetal-to-maternal concentration (F:M) ratios observed studies were much lower than those vivo. In present study, we developed a transplacental pharmacokinetic model using previously reported data. consists maternal intervillous,...
Breast cancer resistance protein (BCRP) is expressed by brain capillary endothelial cells and at the interface between two placental syncytiotrophoblast layers in rodents serves to suppress drug distribution fetus. The purpose of present study determine compare apparent impact a single BCRP molecule on transfer maternal blood-brain barrier pregnant mice different gestation ages. was quantified liquid chromatography-tandem mass spectrometry. Genistein or dantrolene continuously administered...