A5027725425- Peripheral Artery Disease Management
- Vascular Procedures and Complications
- Aortic aneurysm repair treatments
- Sperm and Testicular Function
- Cerebrovascular and Carotid Artery Diseases
- Reproductive Biology and Fertility
- Venous Thromboembolism Diagnosis and Management
- Diagnosis and Treatment of Venous Diseases
- Cardiac, Anesthesia and Surgical Outcomes
- Atherosclerosis and Cardiovascular Diseases
- Lipoproteins and Cardiovascular Health
- Aortic Disease and Treatment Approaches
- Angiogenesis and VEGF in Cancer
- Renal and related cancers
- Cardiac Valve Diseases and Treatments
- Cardiovascular Health and Disease Prevention
- Cell Adhesion Molecules Research
- Systemic Lupus Erythematosus Research
- Stroke Rehabilitation and Recovery
- Coronary Interventions and Diagnostics
- Lymphatic System and Diseases
- Diabetic Foot Ulcer Assessment and Management
- Adenosine and Purinergic Signaling
- Acute Ischemic Stroke Management
- Microbial metabolism and enzyme function
University of Turku
2016-2025
Turku University Hospital
2016-2025
Satakunta Central Hospital
2020-2025
University of Helsinki
2024
King Faisal Specialist Hospital & Research Centre
2024
University Hospital of Bern
2024
University of Bern
2024
Turku University of Applied Sciences
2024
Helsinki University Hospital
2024
Kainuun keskussairaala
2020
The quantitative effects of ethane dimethane sulfonate (EDS), a Leydig cell toxin, on apoptosis in adult rat seminiferous epithelium were studied by the improved transillumination method. Nonradioactive situ end labeling fragmented DNA squash preparations revealed significant increases apoptotic cells stages II-XI, whereas controls showed 0.5-2.3 cells/mm tubule. Seven days post-EDS treatment, highest numbers scored VIIab and VIIcd (74.7 +/- 23.8 61.3 16.0 cells/mm, respectively). suppressed...
beta-Nerve growth factor (NGF) is expressed in spermatogenic cells and has testosterone-downregulated low-affinity receptors on Sertoli suggesting a paracrine role the regulation of spermatogenesis. An analysis stage-specific expression NGF its low affinity receptor during cycle seminiferous epithelium rat revealed mRNA protein at all stages cycle. Tyrosine kinase (trk) encoding an essential component high-affinity was also present stages. In contrast, only found VIIcd VIII cycle, sites...
Activin and inhibin are members of the transforming growth factor-beta (TGF beta) gene family. They expressed in various organ systems, where they possess regulatory functions. Inhibin, activin, TGF beta have been reported to also be adult rat testis. We studied vitro action these factors on premitotic premeiotic DNA synthesis during seminiferous epithelial cycle. Two-millimeter tubule segments were isolated by transillumination-assisted microdissection from stages V, VIIa, VIII-IX, I cycle...
Inguinal incision is the most common vascular surgery and associated with a high rate of surgical site infections (SSIs). The objective this study was to determine whether intradermal suture leads lower SSI compared metal staples. A multicentre, open label, superiority randomised controlled trial conducted from March 2018 until November 2021 in three Finnish hospitals (ClinicalTrials.gov ID: NCT03468621). Patients scheduled elective isolated exposure femoral vessels longitudinal incision,...
To address the possibility that stem cell factor (SCF) is a paracrine regulator of germ development in adult rat testis, stage-specific distribution SCF messenger RNA (mRNA) was investigated with Northern blot and situ hybridization analyses. The highest levels mRNA were found stages II–VI seminiferous epithelial cycle, whereas lowest VII–VIII. Intermediate detected IX–XIV–I cycle. expression gene to be developmentally regulated, pattern followed process Sertoli proliferation differentiation...
Dioxins, e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), use the aryl hydrocarbon receptor (AHR)/aryl nuclear translocator (ARNT) complex to mediate their toxic actions. In addition interaction with environmental pollutants, several transcription factors, steroid receptors, and growth factors are capable interacting AHR/ARNT complex, which suggests a constitutive role for complex. The testis has been reported be among most sensitive organs TCDD exposure. Our experiments revealed...
Orexins, hypothalamic neuropeptides initially involved in the control of food intake and sleep-wake cycle, have recently emerged as pleiotropic regulators different biological systems, including reproductive axis. Besides central actions, peripheral expression functions orexins been reported, prepro-orexin orexin type-1 receptor mRNAs detected testis. However, pattern actions male gonad remain mostly unexplored. In this study, we report analyses on testicular mRNA orexin-A immunoreactivity...
Summary Two techniques have been combined for quantification of apoptotic germ cells in defined stages the cycle seminiferous epithelium: improved transillumination method and nonradioactive in‐situ end‐labelling DNA (ISEL). Segments rat tubules were squashed between a microscope slide coverslip, stage identified under phase‐contrast microscope. After fixation, detected by ISEL scored per 1 mm tubule. In normal testis found all stages, highest frequency occurring XII–XIV (19 cells/mm)....
Rationale: Purinergic signaling plays an important role in inflammation and vascular integrity, but little is known about purinergic mechanisms during the pathogenesis of atherosclerosis humans. Objective: The objective this study to markers a cohort patients with peripheral artery disease. Methods Results: Plasma ATP ADP levels serum nucleoside triphosphate diphosphohydrolase-1 (NTPDase1/CD39) ecto-5′-nucleotidase/CD73 activities were measured 226 stable disease admitted for nonurgent...
Abstract Inflammation plays an important role in the development of atherosclerosis and its complications. Because folate receptor β (FR-β) is selectively expressed on macrophages, FR targeted imaging agent could be useful for assessment atherosclerotic inflammation. We investigated aluminum fluoride-18-labeled 1,4,7-triazacyclononane-1,4,7-triacetic acid conjugated ( 18 F-FOL) detection plaque studied plaques mice, rabbits, human tissue samples using F-FOL positron emission...
A low ankle-brachial index (ABI) has been linked to systemic inflammation and an elevated risk of cardiovascular events, most notably myocardial infarction stroke. Intracranial aneurysms (IAs) share similar factors with other diseases. However, the association between ABI IAs not sufficiently investigated. Our objective was investigate potential connection values prevalence unruptured IAs.
Background Venous leg ulcers (VLUs) affect approximately 1% of the adult population and incur significant morbidity healthcare costs. Endovenous interventions, such as endovenous laser ablation (EVLA) ultrasound-guided foam sclerotherapy (UGFS), have shown benefits in treating venous insufficiency patients with VLUs. However, effect specifically targeting sub-ulcer plexus remains poorly understood. Objective The FINNULCER trial is investigating addition to conventional treatment (EVLA +...
The functional significance of the first intron Col1a1 gene in regulation type I collagen synthesis remains uncertain. A previous study mice established that a mutated allele lacked large fraction intron, but retained sequences required for normal splicing, was subject to an age- and tissue-dependent decrease expression. In this study, we report homozygous deletion are predisposed dissection rupture aorta during their adult life. Aortic not detected autopsies heterozygous animals or...
Changes in the level of gene transcript heat shock protein (hsp)60, a mitochondrial chaperonin, during cycle rat seminiferous epithelium and its cellular localization were studied. The showed cell type-specific expression hsp60. Immunostaining adult testis revealed Sertoli Leydig cells. In germ cells, mitochondria spermatogonia early primary spermatocytes immunoreactive for Mitochondria all other types completely negative Stage-specific hsp60 was determined from pooled segments...
Abstract Given the important role of inflammation and potential association leukocyte trafficking-associated adhesion molecule vascular protein 1 (VAP-1) with atherosclerosis, this study examined whether functional VAP-1 is expressed in atherosclerotic lesions and, if so, it could be targeted by positron emission tomography (PET). First, immunohistochemistry revealed that localized to endothelial cells intra-plaque neovessels human carotid endarterectomy samples from patients recent ischemic...
Critical limb ischemia (CLI) is the advanced stage of peripheral artery disease (PAD) and associated with an extremely poor clinical outcome. In order to understand possible role circulating cytokines outcome CLI we compared cytokine profile patients against intermittent claudication (IC). The levels 48 were examined in 226 consecutive admitted for elective, non-urgent, invasive treatment IC or CLI. PAD patient cohort was evenly distributed between subjects (46.5%) (53.5%). As accustomed...
Clinical management of critical limb-threatening ischaemia (CLTI) is focused on prevention and treatment atherosclerotic arterial occlusions. The role microvascular pathology in disease progression still largely unspecified more importantly not utilized for treatment. aim this explorative study was to characterize the microvasculature CLTI pathology.
Patients needing surgery for peripheral arterial disease (PAD) represent a severe form of atherosclerosis with an overall 5-yr mortality 30% after revascularisation. The aetiology poor post-operative clinical outcome in these high-risk patients is not fully established. Obstructive sleep apnoea (OSA) associated and independent risk factor fatal nonfatal cardiac events. Here, we determine the prevalence undiagnosed OSA homogenous group PAD undergoing subinguinal surgical 82 consecutive (mean...
Rationale: Ischemic injuries remain a leading cause of mortality and morbidity worldwide, restoration functional blood perfusion is vital to limit tissue damage support healing. Objective: To reveal novel role macrophages in reestablishment following ischemic injury that can be targeted improve restoration. Methods Results: Using intravital microscopy hindlimb muscle mice, confocal human tissues from amputated legs, we found accumulated perivascularly muscles, where they expressed high...
The effects of FSH on stage-specific apoptosis and DNA synthesis in the adult rat seminiferous epithelium were studied vitro. Seminiferous tubular segments from stages I, V, VIIa, VIII-IX cultured for 24, 48, 72 h different concentrations FSH. Apoptotic cells detected by situ end labeling strands quantified squash preparations. After 48 culture, a concentration 2 ng/ml prevented early (steps 1-3) spermatids. In stage tubules h, decreased pachytene spermatocytes. An apoptotic type cell death...