A5051684590- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Yersinia bacterium, plague, ectoparasites research
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Infective Endocarditis Diagnosis and Management
- Bacterial Genetics and Biotechnology
- Streptococcal Infections and Treatments
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Pharmacological Effects of Natural Compounds
- Advanced Fluorescence Microscopy Techniques
- Bacillus and Francisella bacterial research
- Plant-based Medicinal Research
- Whipple's Disease and Interleukins
- Biochemical and Structural Characterization
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Resistance in Bacteria
- Advanced Electron Microscopy Techniques and Applications
- Transgenic Plants and Applications
- Bone and Dental Protein Studies
- Toxin Mechanisms and Immunotoxins
- Leptospirosis research and findings
- Enterobacteriaceae and Cronobacter Research
- Mycobacterium research and diagnosis
Universität Hamburg
2013-2023
University Medical Center Hamburg-Eppendorf
2013-2023
Institute of Medical Microbiology and Hygiene
2007
Early and adequate antimicrobial therapy has been shown to improve the clinical outcome in bloodstream infections (BSI). To provide rapid pathogen identification for targeted treatment, we applied matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry fingerprinting bacteria directly recovered from blood culture bottles. A total 304 aerobic anaerobic cultures, reported positive by a Bactec 9240 system, were subjected parallel differential centrifugation with...
Five Klebsiella pneumoniae isolates with reduced susceptibility to tigecycline (MIC, 2 microg/ml) were analyzed. A gene homologous ramR of Salmonella enterica was identified in pneumoniae. Sequencing the nonsusceptible strains revealed deletions, insertions, and point mutations. Transformation mutants wild-type genes, but not mutant restored repressed overexpression ramA acrB. Thus, this study reveals a molecular mechanism for resistance
In 2011 northern Germany experienced a large outbreak of Shiga-Toxigenic Escherichia coli O104:H4. The amount samples sent to microbiology laboratories for epidemiological assessment highlighted the importance fast and inexpensive typing procedures. We have therefore evaluated applicability MALDI-TOF mass spectrometry based strategy strain identification.Specific peaks in strain's spectrum were identified by comparative analysis archived pre-outbreak spectra that had been acquired routine...
Hypertoxigenic Streptococcus pyogenes emm1 lineage M1
An outbreak caused by Escherichia coli serotype O104:H4 strains has been affecting northern Germany since May 2011, with 3,222 patients infected and 39 of them dead 18 June ([4][1]). Around 25% the developed hemolytic-uremic syndrome (HUS) ([1][2], [3][3]), a rate which is much higher
Whipple's disease (WD) is a rare infection with Tropheryma whipplei that fatal if untreated. Diagnosis challenging and currently based on invasive sampling. In case of WD diagnosed from kidney biopsy, we observed morphologically-intact bacteria within the glomerular capsular space tubular lumens. This raised questions whether renal filtration common in polymerase chain reaction (PCR) testing urine might serve as diagnostic test for WD.We prospectively investigated samples 12 newly-diagnosed...
Staphylococcus aureus is among the most common pathogens isolated from blood cultures in Ghana; yet epidemiology of infections rural settings poorly described. This study aims to investigate antimicrobial susceptibility and clonal diversity S. causing bloodstream two hospitals Ashanti Region, Ghana. Blood were performed for all febrile patients (≥37.5 °C) on hospital admission. Antibiotic testing isolates was carried out by VITEK 2 system. Multiplex polymerase chain reaction (PCR) used...
Type III secretion systems (T3SSs) are essential virulence factors of numerous bacterial pathogens. Upon host cell contact the T3SS machinery—also named injectisome—assembles a pore complex/translocon within membranes that serves as an entry gate for effectors. Whether and how translocons physically connected to injectisome needles, whether their phenotype is related level effector translocation which target trigger formation have remained unclear. We employed superresolution fluorescence...
Abstract The resolution achievable with the established super-resolution fluorescence nanoscopy methods, such as STORM or STED, is in general not sufficient to resolve protein complexes even individual proteins. Recently, minimal photon flux (MINFLUX) has been introduced that combines strengths of STED and can achieve a localization precision less than 5 nm. We generally applicable workflow for MINFLUX imaging applied it first time bacterial molecular machine situ , i.e., injectisome...
We isolated a clinical Escherichia coli strain with an antimicrobial resistance phenotype characteristic for the expression of AmpC beta-lactamase. Molecular methods revealed novel, plasmid-localized variant CMY-2 substitution valine 231 serine (V231S), which was designated CMY-42. Like CMY-2-like beta-lactamase CMY-30, carrying V231G, CMY-42 displayed increased activity toward expanded spectrum cephalosporins. This finding supports hypothesis that bulky side chain at position (Ambler's 211)...
Pathogenic Yersinia spp. translocate the effectors YopT, YopE, and YopO/YpkA into target cells to inactivate Rho family GTP-binding proteins block immune responses. Some also secrete protein activator cytotoxic necrotizing factor-Y (CNF-Y), but it has been unclear how bacteria may benefit from activation. We show here that CNF-Y increases Yop translocation in enterocolitica-infected up 5-fold. strongly activated RhoA delayed time Rac1 Cdc42, when individually expressed, constitutively active...
Background: Globally, Staphylococcus aureus is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance S. to locally available antibiotics increasing but the molecular basis population structure in particular chronic wounds are poorly described. However, this information essential understand underlying mechanisms spread resistant clones. We therefore subjected 28 isolates from infected rural area Ghana whole genome sequencing....
Yersinia enterocolitica employs a type three secretion system (T3SS) to translocate immunosuppressive effector proteins into host cells. To this end, the T3SS assembles translocon/pore complex composed of translocator YopB and YopD in cell membranes serving as an entry port for effectors. The translocon is formed -containing pre-phagosomal compartment that connected extracellular space. As phagosome matures, membrane damage it causes are recognized by cell-autonomous immune system. We...
Staphylococcus epidermidis is a leading pathogen in implant-associated hospital infections. The pathogenesis critically depends on bacterial binding to ECM components, specifically fibronectin (Fn). cell surface-localized, 1-MDa extracellular matrix protein (Embp) essentially characterized by 10 F- and 40 FG-repeats. These repetitive units, each two α-helical bundles, organize themselves rigid, elongated form. Embp binds preferentially surface-localized but not soluble Fn, with both...
Many gram-negative bacteria including pathogenic Yersinia spp. employ type III secretion systems to translocate effector proteins into eukaryotic target cells. Inside the host cell manipulate cellular functions benefit of bacteria. To better understand control during interaction, sensitive and accurate assays measure translocation are required. We here describe application an assay based on fusion a enterocolitica protein fragment (Yersinia outer protein; YopE) with TEM-1 beta-lactamase for...
Abstract Type 3 secretion systems (T3SS) are essential virulence factors of numerous bacterial pathogens and inject effector proteins into host cells. The needle-like T3SS machinery consists more than 20 components, has a length around 100 nm diameter up to 30 according EM studies. Its intrabacterial components highly dynamic in permanent exchange with other structures. Therefore, temporally spatially resolved visualization the using fluorescence microscopy techniques been challenging. In...
Abstract Purpose Hypertoxigenic Streptococcus pyogenes emm 1 lineage M1 UK has recently been associated with upsurges of invasive infections and scarlet fever in several countries but whole-genome sequencing surveillance data from Germany is lacking. We here aimed at exploring recent isolates our laboratory a German tertiary care center for the presence . Methods Whole-genome was employed to characterize collection 47 consecutive non-copy recovered blood cultures (n = 21) tissue samples 26)...
Abstract Yersinia enterocolitica employs a type three secretion system (T3SS) to translocate immunosuppressive effector proteins into host cells. To this end, the T3SS assembles translocon/pore complex composed of translocator YopB and YopD in cell membranes serving as an entry port for effectors. The translocon is formed -containing pre-phagosomal compartment that connected extracellular space. As phagosome matures, membrane damage it causes are recognized by cell-autonomous immune system....
Many gram-negative bacteria including pathogenic Yersinia spp. employ type III secretion systems to translocate effector proteins into eukaryotic target cells. Inside the host cell manipulate cellular functions benefit of bacteria. To better understand control during interaction, sensitive and accurate assays measure translocation are required. We here describe application an assay based on fusion a enterocolitica protein fragment (Yersinia outer protein; YopE) with TEM-1 beta-lactamase for...