A5043787041- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Infection Control in Healthcare
- Bacterial Identification and Susceptibility Testing
- Infection Control and Ventilation
- Medical Device Sterilization and Disinfection
- Listeria monocytogenes in Food Safety
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Streptococcal Infections and Treatments
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Resistance in Bacteria
- Indoor Air Quality and Microbial Exposure
- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 detection and testing
- Bacillus and Francisella bacterial research
- Plant-Microbe Interactions and Immunity
- Bacterial Infections and Vaccines
- Antifungal resistance and susceptibility
- Pneumonia and Respiratory Infections
- Building Energy and Comfort Optimization
- Antimicrobial agents and applications
- Fungal Infections and Studies
- Ocular Infections and Treatments
Universität Hamburg
2009-2023
University Medical Center Hamburg-Eppendorf
2009-2023
Institut für Medizinische Informatik, Biometrie und Epidemiologie
2009-2015
GTx (United States)
2006
The extent of monkeypox virus environmental contamination surfaces is unclear. We examined in rooms occupied by two patients on their fourth hospitalisation day. Contamination with up to 10 5 viral copies/cm 2 inanimate was estimated PCR and the successfully isolated from more than 6 copies. These data highlight importance strict adherence hospital staff recommended protective measures. If appropriate, pre-exposure or early post-exposure vaccination should be considered for individuals at risk.
Virulence of nosocomial pathogen Staphylococcus epidermidis is essentially related to formation adherent biofilms, assembled by bacterial attachment an artificial surface and subsequent production a matrix that mediates interbacterial adhesion. Growing evidence supports the idea proteins are functionally involved in S. biofilm accumulation. We found 1585v overexpression 460 kDa truncated isoform extracellular matrix-binding protein (Embp) necessary for formation. Embp giant...
The presence of coronaviruses on surfaces in the patient environment is a potential source indirect transmission. Manual cleaning and disinfection measures do not always achieve sufficient removal surface contamination. This increases importance automated solutions context final rooms hospital setting. Ozone highly effective disinfectant which, combined with high humidity, an agent against respiratory viruses. Current devices allow continuous nebulization for room humidity as well ozone...
Summary Biofilm formation is essential for S taphylococcus epidermidis pathogenicity in implant‐associated infections. Nonetheless, large proportions of invasive isolates fail to form a biofilm vitro . We here tested the hypothesis that this apparent paradox related existence superimposed regulatory systems suppressing multicellular life style Transposon mutagenesis clinical significant but biofilm‐negative 1585 was used isolate positive mutant carrying Tn 917 insertion sarA , chief...
A universal PCR assay for bacteria and fungi detected meningitis pathogens in 65% of 20 cerebrospinal fluid (CSF) samples from patients with suspected central nervous system (CNS) infections compared to a 35% detection rate by culture and/or microscopy methods. Thus, the can improve diagnosis infective when standard methods provide negative result.
Hospitals are one of the main reservoirs multi-resistant Enterobacterales (MRE). As MRE resistant to most frequently used antibiotics, therapy for patients with infections is challenging. It has been previously described that from hospital wastewater can pass into municipal and even surface water. In this study, we investigated diversity epidemiology in a large tertiary care hospital. Wastewater samples were collected four-day period tested presence 3rd gen. cephalosporins. Representative...
Virulence of the nosocomial pathogen Staphylococcus epidermidis is crucially linked to formation adherent biofilms on artificial surfaces. Biofilm assembly significantly fostered by production a bacteria derived extracellular matrix. However, matrix composition, spatial organization, and relevance specific molecular interactions for integration bacterial cells into multilayered biofilm community are not fully understood. Here we report function novel 18 kDa Small basic protein (Sbp) that was...
Infections associated with indwelling medical devices are a major problem in modern medicine, affecting millions of patients worldwide each year. Staphylococci, especially Staphylococcus aureus and epidermidis, the most characteristic causative organisms isolated context device-related infections. The tight pathogenetic association between foreign-body implantation staphylococcal infection is related to their capability establish multilayered, highly structured biofilms on artificial...
Admission to a room previously occupied by patients carrying environmentally robust pathogens implies an increased risk of acquiring those pathogens. Therefore, 'No-touch' automated disinfection systems, including devices based on UV-C irradiation, are discussed improve terminal cleaning. It is still unclear if clinical isolates relevant behave differently under irradiation compared laboratory strains used in the approval process procedures. In this study we analysed susceptibility well...
Vancomycin-resistant Enterococcus faecium and faecalis (VRE) are a common cause of healthcare-associated infections. Whole genome sequencing-based typing methods yield the highest discriminatory power for outbreak surveillance in hospital. We analysed clonal composition enteric VRE populations at-risk patients over several weeks to characterise population diversity dynamics.Five bone marrow transplant recipients (three colonised with vanA-positive isolates, two vanB-positive isolates)...
To evaluate the effectiveness of automated room decontamination devices, a common aerosolized hydrogen peroxide (aHP) as well recent gaseous ozone-based device, which produces disinfectant reagent without need consumables, were tested under real-life conditions. Twenty-two contaminated surfaces positioned in different areas patient with adjacent bathroom and anteroom. Following process bacteria recovered reduction factors calculated after performing quantitative culture. manufactures...
Worldwide observations revealed increased frequencies of multi-resistant Enterobacterales and resistance genes in hospital wastewater compared to any other type wastewater. Despite the description clonal lineages possibly adapted wastewater, little is known about long term persistence as well evolution these lineages.In this study, isolates different species from a tertiary care were investigated with 2.5 years distance. Whole Genome Sequencing (WGS) gene identification performed for E....
EDIN-B (epidermal cell differentiation inhibitor-B; also termed C3Stau) is an exotoxin of Staphylococcus aureus which ADP-ribosylates and inactivates Rho GTP binding proteins. The gene ( edin-B ) the for exfoliative toxin D etd make up central part a recently described pathogenicity island. Here we evaluated prevalence genetic organization edin-B/etd island in invasive S. isolates, characterized transcription production using artificial constructs transduced strains RN6390 Newman. We found...
Pigmentation, catalase activity and biofilm formation are virulence factors that cause resistance of
Abstract Background Acinetobacter baumannii is an emerging multiresistant Gram-negative rod, which has caused multiple hospital outbreaks. A. can display a high ability to survive on inanimate surfaces. Therefore, cleaning and disinfection important part in the prevention of transmission. No-touch room decontamination performed with increasing frequency reach more standardization cleaning. In this study, we investigated efficacy automated using ozone (Sterisafe™ pro) against under complex...
The potential impact of music-making on air quality around musicians was inferred at the outset SARS-CoV-2 pandemic from measurements individual musical instruments and theoretical considerations. However, it is unclear to what extent playing together in an orchestra under optimal ventilation conditions really increases infection risks for musicians. In this study, changes indoor were assessed by measuring common parameters, i.e., temperature, relative humidity, carbon dioxide, along with...
Antimicrobial materials or surfaces are advertised as part of infection prevention bundles. However, the efficacy such antimicrobial has not been sufficiently investigated in hospitals. In this study, activity examination gloves with light-activated properties against Gram-positive microorganisms was modelling real live conditions.In a standardized experimental set-up dry and realistic contamination, claiming light dependent organisms were tested comparison conventional gloves. All...