A5100695515- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Microfluidic and Bio-sensing Technologies
- Nanopore and Nanochannel Transport Studies
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Microfluidic and Capillary Electrophoresis Applications
- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Gold and Silver Nanoparticles Synthesis and Applications
- Wireless Body Area Networks
- Carcinogens and Genotoxicity Assessment
- Heat shock proteins research
- Neuroscience and Neural Engineering
- Endoplasmic Reticulum Stress and Disease
- S100 Proteins and Annexins
- Micro and Nano Robotics
- Sepsis Diagnosis and Treatment
- Nanofabrication and Lithography Techniques
- Biosensors and Analytical Detection
- DNA Repair Mechanisms
- RNA Research and Splicing
- Immune Response and Inflammation
- Retinal Development and Disorders
- 3D Printing in Biomedical Research
Beijing Institute of Technology
2020-2025
First Affiliated Hospital of Zhengzhou University
2023
Zhengzhou University
2023
Peking University
2020
National Health and Family Planning Commission
2020
Harbin Medical University
2019
First Affiliated Hospital of Harbin Medical University
2019
Jiangsu University
2018
A CRISPR-based technique achieves projection- and function-specific gene editing in the rat brain.
Micromanipulation is crucial for operating and analyzing microobjects in advanced biomedical applications. However, safe, low-cost, multifunctional micromanipulation bio-objects across scales modalities remains inaccessible. Here, we propose a versatile method driven by acoustic gas-liquid-solid interactions, named μSonic-hand. The bubble contained at the end of micropipette surrounding liquid form gas-liquid multiphase system susceptible to waves. Driven piezoelectric transducer,...
Using multiple parallel folding to aid the DOS into TDN, which can respond epithelial cell adhesion molecules (EpCAM) for a controlled conformational change.
In recent decades, cell immobilization using microfluidic chips has facilitated significant advancements in biological analyses at the single-cell level. However, efficient capture of multiple cells as a cluster adjustable quantities for cell–cell interaction not been achieved. this paper, aiming to monitor level, we proposed novel method on basis passive hydrodynamics so that different patterns could be generated. Experiments were conducted characterize key geometric parameters chip...
Synthetic membrane nanopores made of DNA are promising systems to sense and control molecular transport in biosensing, sequencing, synthetic cells. Lumen-tunable nanopore like the natural ion channels systematically increasing lumen size have become long-standing desires developing nanopores. Here, we design a triangular with large tunable lumen. It allows in-situ transition from expanded state contracted without changing its stable shape, vice versa, which specific bindings as stimuli...
Aims Stress granules (SGs) are transient cytoplasmic mRNA and protein complexes that form in eukaryotic cells under stress. SGs related to multiple diseases, but there no reports of the existence atrial fibrillation (AF). Methods results Cell models AF were established by field stimulation at 600 times per minute. HL-1 cells, cardiomyocytes cardiac fibroblasts transfected with G3BP1-cDNA plasmid Lipofectamine 2000. The presence was detected immunofluorescence analysis against...
DNA origami-engineered nanostructures are widely used in biomedical applications involving transmembrane delivery. Here, we propose a method to enhance the capability of origami sheets by changing their configuration from two-dimensional three-dimensional. Three designed and constructed, including rectangular sheet, tube, tetrahedron. The latter two variants sheet with three-dimensional morphologies achieved through one-step folding multi-step parallel separately. design feasibility...
Sepsis is a clinical syndrome with high mortality. Subtype identification in sepsis meaningful for improving the diagnosis and treatment of patients. The purpose this research was to identify subtypes using RNA-seq datasets further explore key genes that were deregulated during development sepsis.The GSE95233 GSE13904 obtained from Gene Expression Omnibus database. Differential analysis gene expression matrix performed between patients healthy controls. Intersection differentially expressed...
<title>Abstract</title> Synthetic membrane nanopores made of DNA are promising systems to sense and control molecular transport in biosensing, sequencing, synthetic cells. Dynamically gating cargo like the natural ion channels systematically increasing lumen size have become long-standing desires developing nanopores. Here, we design a triangular nanopore with large dynamically-gated lumen. It can switch between expanded contracted states without changing its stable shape, whereby specific...
Abstract-The mechanical characterization of cell is important for knowing the physiological state and studying diseases organism. While many approaches are available measuring cellular elasticity, distinguishing stiffness variation among different areas still a challenge. In this paper, we reported method to modify spherical atomic force microscopy (AFM) tip accurate measurement Young's modulus in several on single adherent living cancer cells (Hela cells). The micrometer size spheres were...
In the biomicromanipulation field, single-cell manipulation is receiving attention due to its potential applications in many aspects of biomedicine. Cell injection, as a typical cell micromanipulation technique, devoted introducing foreign substances into living cells by penetrating fine micropipette membranes. Direct access intracellular environment, especially nucleus, provides an ideal way gain insight dynamic processes cells. This paper presents comparison controlled penetrations two...