A5083412877- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Cancer, Hypoxia, and Metabolism
- Genetic Syndromes and Imprinting
- Angiogenesis and VEGF in Cancer
- Renal Diseases and Glomerulopathies
- Neuroendocrine regulation and behavior
- Cancer Treatment and Pharmacology
- Eicosanoids and Hypertension Pharmacology
- Cellular Mechanics and Interactions
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
- Coronary Interventions and Diagnostics
- Atherosclerosis and Cardiovascular Diseases
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Biomedical Research and Pathophysiology
- Chronic Kidney Disease and Diabetes
- Congenital heart defects research
- Kruppel-like factors research
- MicroRNA in disease regulation
- Neuroscience of respiration and sleep
- Organic and Inorganic Chemical Reactions
- interferon and immune responses
- Developmental Biology and Gene Regulation
University of Sheffield
2013-2024
Queen Mary University of London
2023-2024
William Harvey Research Institute
2023-2024
The General Hospital of Heraklion "Venizeleio-Pananio"
2023
Insigneo
2017-2022
Hellenic Mediterranean University
2022
Centre for Life
2019
Georgia Institute of Technology
2017
Ludwig-Maximilians-Universität München
2017
German Centre for Cardiovascular Research
2017
Objective— Atherosclerosis develops near branches and bends of arteries that are exposed to low shear stress (mechanical drag). These sites characterized by excessive endothelial cell (EC) proliferation inflammation promote lesion initiation. The transcription factor HIF1α (hypoxia-inducible 1α) is canonically activated hypoxia has a role in plaque neovascularization. We studied the influence on activation contribution this noncanonical pathway Approach Results— Quantitative polymerase chain...
ABSTRACT Endothelial cell (EC) sensing of fluid shear stress direction is a critical determinant vascular health and disease. Unidirectional flow induces EC alignment homeostasis, whereas bidirectional has pathophysiological effects. ECs express several mechanoreceptors that respond to flow, but the mechanism for poorly understood. We determined, by using in vitro systems magnetic tweezers, β1 integrin key sensor force because it activated unidirectional, not bidirectional, shearing forces....
Hypoxia followed by reoxygenation promotes inflammation activating nuclear factor κB transcription factors in endothelial cells (ECs). This process involves modification of the signaling intermediary tumor necrosis receptor-associated 6 with polyubiquitin chains. Thus, cellular mechanisms that suppress ubiquitination are potential therapeutic targets to reduce hypoxic tissues.In this study, we tested hypothesis activation response hypoxia-reoxygenation can be influenced Cezanne, a...
Endothelial cell (EC) sensing of disturbed blood flow triggers atherosclerosis, a disease arteries that causes heart attack and stroke, through poorly defined mechanisms. The Notch pathway plays central role in vessel growth homeostasis, but its potential has not been previously studied. Here, we show using porcine murine cultured human coronary artery EC activates the JAG1-NOTCH4 signaling pathway. Light-sheet imaging revealed enrichment JAG1 NOTCH4 atherosclerotic plaques, EC-specific...
Transforming growth factor-β (TGF-β) is an inducer of type I collagen, and uncontrolled collagen production leads to tissue scarring organ failure. Here we hypothesize that uncovering a molecular mechanism enables us switch off may prove beneficial in treating fibrosis. For the first time, our knowledge, provide evidence CUX1 acts as negative regulator TGF-β potent inhibitor transcription. We show CUX1, CCAAT displacement protein, associated with reduced expression both vivo vitro. enhancing...
Abstract Autosomal dominant polycystic kidney disease (ADPKD) is the most common renal genetic disorder, however it still lacks a cure. The discovery of new therapies heavily depends on understanding key signalling pathways that lead to ADPKD. JA nus K inase and S ignal T ransducers A ctivators ranscription (JAK/STAT) pathway aberrantly activated contributes ADPKD pathogenesis via enhancing epithelial proliferation. Yet mechanisms underlying upregulation JAK/STAT activity in this context...
Atherosclerosis develops near branches and bends of arteries that are exposed to disturbed blood flow which exerts low wall shear stress (WSS). These mechanical conditions alter endothelial cells (EC) by priming them for inflammation inducing turnover. Homeobox (Hox) genes developmental involved in the patterning embryos along their anterior-posterior proximal-distal axes. Here we identified Hox regulated WSS investigated functions adult arteries.EC were isolated from inner (low WSS) outer...
Clear cell renal carcinoma (ccRCC) represents the most common kidney cancer worldwide. Increased proliferation associated with abnormal microRNA (miRNA) regulation are hallmarks of carcinogenesis. Ankyrin repeat and single KH domain 1 (ANKHD1) is a highly conserved protein found to interact core pathways in Drosophila; however, its involvement RCC completely unexplored. Quantitative PCR studies coupled large-scale genomics data sets demonstrated that ANKHD1 significantly up-regulated kidneys...
Atherosclerotic plaques form unevenly due to disturbed blood flow, causing localized endothelial cell (EC) dysfunction. Obesity exacerbates this process, but the underlying molecular mechanisms are unclear. The transcription factor EPAS1 (HIF2A) has regulatory roles in endothelium, its involvement atherosclerosis remains unexplored. This study investigates potential interplay between EPAS1, obesity, and atherosclerosis.
CCN2, a secreted profibrotic protein, is highly expressed in diabetic nephropathy (DN) and implicated its pathogenesis; however, the actions of CCN2 DN remain elusive. We previously demonstrated that triggers signaling via tropomyosin receptor kinase A (TrkA). Trace expression TrkA found normal kidneys, but elevated several nephropathies; yet role unexplored. In this study we show de novo human murine DN. go on to molecular mechanisms leading activation it involves hypoxia, as by...
Cytokine receptors often act via the Janus kinase and signal transducer activator of transcription (JAK/STAT) pathway to form a signalling cascade that is essential for processes such as haematopoiesis, immune responses tissue homeostasis. In order transduce ligand activation, cytokine must dimerise. However, mechanisms regulating their dimerisation are poorly understood. better understand receptor levels, activity dimerisation, we analysed highly conserved JAK/STAT in Drosophila, which acts...
In the enhancer region of human type I collagen alpha 2 (COL1A2) gene, we identified cis-elements for transcription factor CUX1. However, role CUX1 in fibrosis remains unclear. Here investigated regulation COL1 expression and delineated mechanisms underlying COL1A2 by systemic sclerosis (SSc) lung fibroblasts. The binding to was assessed using electrophoretic mobility shift assays after treatment with transforming growth (TGF)-β. Subsequently, protein levels isoforms were determined Western...
ABSTRACT The ability of endothelial cells (EC) to sense blood flow direction is a critical determinant vascular health and disease. Unidirectional induces EC alignment homeostasis, whereas bidirectional has pathophysiological effects. express several mechanoreceptors that can respond fluid (shear stress) but the mechanism for sensing shearing force poorly understood. We observed using in vitro systems magnetic tweezers β1 integrin key sensor because it activated by unidirectional not forces....
This data article contains complementary figures related to the research entitled, "Transforming growth factor-β-induced CUX1 isoforms are associated with fibrosis in systemic sclerosis lung fibroblasts" (Ikeda et al. (2016) [2], http://dx.doi.org/10.1016/j.bbrep.2016.06.022), which presents that TGF-β increased binding proximal promoter and enhancer of COL1A2 regulated COL1. Further, scleroderma (SSc) diffuse alveolar damage sections, localized within α- smooth muscle actin (α-SMA) positive...