A5047404158- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Renal and related cancers
- Pancreatic function and diabetes
- Neuroscience and Neural Engineering
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
- Nanowire Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Plant Virus Research Studies
- Genetics, Aging, and Longevity in Model Organisms
- 3D Printing in Biomedical Research
- Characterization and Applications of Magnetic Nanoparticles
- Atherosclerosis and Cardiovascular Diseases
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Bacteriophages and microbial interactions
- Nanoparticle-Based Drug Delivery
- Genetics and Neurodevelopmental Disorders
- Systemic Lupus Erythematosus Research
- Nanocluster Synthesis and Applications
Pennsylvania State University
2016-2024
The San Raffaele Telethon Institute for Gene Therapy
2022-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2022-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2022-2024
Case Western Reserve University
2013-2016
The underlying cause of major cardiovascular events, such as myocardial infarctions and strokes, is atherosclerosis. For accurate diagnosis this inflammatory disease, molecular imaging required. Toward goal, we sought to develop a nanoparticle-based, high aspect ratio, molecularly targeted magnetic resonance (MR) contrast agent. Specifically, engineered the plant viral nanoparticle platform tobacco mosaic virus (TMV) target vascular cell adhesion molecule (VCAM)-1, which highly expressed on...
Drug delivery systems are required for drug targeting to avoid adverse effects associated with chemotherapy treatment regimes. Our approach is focused on the study and development of plant virus-based materials as systems; specifically, this work focuses tobacco mosaic virus (TMV). Native TMV forms a hollow, high aspect-ratio nanotube measuring 300 × 18 nm 4 nm-wide central channel. Heat-transformation can be applied yielding spherical nanoparticles (SNPs) ~ 50 in size. While bioconjugate...
Abstract Human pluripotent stem cells (hPSCs) offer tremendous promise in tissue engineering and cell-based therapies due to their unique combination of two properties: pluripotency unlimited proliferative capacity. However, directed differentiation hPSCs clinically relevant cell lineages is needed achieve the goal hPSC-based therapies. This requires a deep understanding how signaling pathways converge on nucleus control ability dissect gene function temporal manner. Here, we report use...
During embryonic development, blood cells emerge from specialized endothelial cells, named haemogenic (HECs). As HECs are rare and only transiently found in early developing embryos, it remains difficult to distinguish them cells. Here we performed transcriptomic analysis of 28- 32-day human embryos observed that the expression Fc receptor CD32 (FCGR2B) is highly enriched cell population contains HECs. Functional analyses using pluripotent stem cell-derived revealed robust multilineage...
Glioblastoma (GBM), the deadliest form of brain cancer, presents long-standing problems due to its localization. Chimeric antigen receptor (CAR) T cell immunotherapy has emerged as a powerful strategy treat cancer. IL-13-receptor-α2 (IL13Rα2), present in over 75% GBMs, been recognized an attractive candidate for anti-glioblastoma therapy. Here, we propose novel multidisciplinary approach target tumors using combination fluorescent, therapeutic nanoparticles and CAR cells modified with...
Shaping contrast agents for macrophage imaging.
Human pluripotent stem cell (hPSC)-derived pancreatic progenitors (PPs) provide promising therapies for type 1 diabetes. Current PP differentiation requires a high amount of Activin A during the definitive endoderm (DE) stage, making it economically difficult commercial ventures. Here we identify dose-dependent role Wnt signaling in controlling DE without A. While high-level activation induces mesodermal formation, low-level by small-molecule inhibitor glycogen synthase kinase 3 is...
Transcription factors (TFs) are pivotal in guiding stem cell behavior, including their maintenance and differentiation. Using single-cell RNA sequencing, we investigated TFs expressed endothelial progenitors (EPs) derived from human pluripotent cells (hPSCs) identified upregulated expression of SOXF SOX7, SOX17, SOX18 the EP population. To test whether overexpression these increases differentiation efficiency, established inducible hPSC lines for each factor found only SOX17 robustly...
Genetically encoded calcium indicator (GCaMP) proteins have been reported for imaging cardiac cell activity based on intracellular transients. To bring human pluripotent stem (hPSC)-derived cardiomyocytes (CMs) to the clinic, it is critical evaluate functionality of CMs. Here, we show that GCaMP6s-expressing hPSCs can be generated and used CM characterization. By leveraging CRISPR-Cas9 genome editing tools, a knockin line constitutively expresses GCaMP6s, an ultrasensitive sensor protein. We...
Abstract Human pluripotent stem cells (hPSCs) offer tremendous promise in tissue engineering and cell-based therapies because of their unique combination two properties: pluripotency a high proliferative capacity. To realize this potential, development efficient hPSC differentiation protocols is required. In work, sex-based differences are identified GSK3 inhibitor based endothelial progenitor protocol. While male hPSCs efficiently differentiate into CD34 + CD31 progenitors upon inhibition,...
Abstract Proper cell‐cycle progression is essential for the self‐renewal and differentiation of human pluripotent stem cells (hPSCs). The fluorescent ubiquitination‐based indicator (FUCCI) has allowed dual‐color visualization G 1 S/G 2 /M phases in various dynamic models, but its application hPSCs not widely reported. In addition, lineage‐specific FUCCI reporters have yet been developed to analyze complex tissue‐specific during hPSC differentiation. Desiring a robust tool spatiotemporal...
The identification and characterization of stem cells, especially human embryonic has revolutionized the field developmental biology by providing an <em>in vitro</em> system to study development. In addition, reprogramming adult cells from patients into cell-like state using induced pluripotent cell (iPSC) technology can potentially generate unlimited source tissue carrying genetic mutations that caused or facilitated disease development, unprecedented possibilities model in culture dish. To...
Abstract Transcription factors (TFs) play critical roles in stem cell maintenance and differentiation. Using single RNA sequencing, we investigated TFs expressed hemogenic endothelial (HE) progenitors differentiated from human pluripotent cells (hPSCs) identified upregulated expression of SOXF SOX7, SOX17 , SOX18 the HE population. To test whether overexpression these increases differentiation efficiency, established inducible hPSC lines found only improved Temporal analysis further revealed...
Human pluripotent stem cells (hPSCs)-derived pancreatic progenitors (PPs) provide promising cell therapies for Type I Diabetes (T1D). Current PP differentiation requires high amount of Activin A during definitive endoderm (DE) stage, making it economically difficult commercial ventures. Here we identify a dose-dependent role Wnt signaling in controlling DE without A. While high-level activation induces mesodermal formation, low-level by small-molecule inhibitor glycogen synthase kinase...