A5003478468- Viral-associated cancers and disorders
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Autoimmune and Inflammatory Disorders Research
- Eosinophilic Disorders and Syndromes
- T-cell and Retrovirus Studies
- Polyomavirus and related diseases
- Herpesvirus Infections and Treatments
- Histiocytic Disorders and Treatments
- CNS Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Hematopoietic Stem Cell Transplantation
- Kawasaki Disease and Coronary Complications
- Organ Transplantation Techniques and Outcomes
- Oral and gingival health research
- Mast cells and histamine
- CAR-T cell therapy research
- Systemic Lupus Erythematosus Research
- Autoimmune Bullous Skin Diseases
- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- RNA regulation and disease
- Infectious Encephalopathies and Encephalitis
National Center For Child Health and Development
2015-2024
Saitama Medical University Hospital
2017
Yokohama City University
2017
Tokyo Medical and Dental University
1999-2017
Tokai University
2014
Wellcome Centre for Cell Biology
2014
Kitasato University
2014
University of Edinburgh
2014
Hiroshima University
2014
Japan Science and Technology Agency
2014
The functional human immune system, including T, B, and natural killer lymphocytes, is reconstituted in NOD/Shi-scid/IL-2Rgamma(null) (NOG) mice that receive hematopoietic stem cell transplants. Here, we show these humanized can recapitulate key aspects of Epstein-Barr virus (EBV) infection humans. Inoculation with approximately 1 x 10(3) TD(50) (50% transforming dose) EBV caused B lymphoproliferative disorder, histopathological findings latent gene expression remarkably similar to...
Systemic chronic active Epstein-Barr virus infection (sCAEBV) was defined as a T- or NK-cell neoplasm in the 2017 World Health Organization (WHO) classification. To clarify clinical features of sCAEBV under this classification and review effects chemotherapy, we performed nationwide survey Japan from 2016 through 2018 patients with newly diagnosed January 2003 March 2016. One hundred cases were evaluated. The aged 1 to 78 years (median, 21) included 53 males 47 females. Spontaneous...
Humanized NOD/Shi-scid/interleukin-2Rgamma(null) (NOG) mice with full T cell development had significantly longer life span after Epstein-Barr virus (EBV) infection, compared those minimal development. Removing CD3(+) or CD8(+) cells from EBV-infected humanized by administration of anti-CD3 anti-CD8 antibodies reduced their span. obtained suppressed the outgrowth autologous B isolated uninfected and inoculated EBV in vitro. These results indicate that NOG are capable cell-mediated control...
Epstein-Barr virus (EBV) has been implicated in the pathogenesis of rheumatoid arthritis (RA) on basis indirect evidence, such as its presence affected joint tissues, antigenic cross reactions between EBV and human proteins, elevated humoral cellular anti-EBV immune responses patients. Here we report development erosive closely resembling RA humanized mice inoculated with EBV. Human system components were reconstituted NOD/Shi-scid/IL-2Rγnull (NOG) strain by transplantation CD34+...
Epstein-Barr virus (EBV) has been detected in several T- and NK-cell neoplasms such as extranodal NK/T-cell lymphoma nasal type, aggressive leukemia, EBV-positive peripheral T-cell lymphoma, systemic of childhood, chronic active EBV infection (CAEBV). However, how this contributes to lymphomagenesis T or NK cells remains largely unknown. Here, we examined NF-κB activation cell lines, SNT8, SNT15, SNT16, SNK6, primary clonally proliferating T/NK obtained from the blood patients with CAEBV....
Epstein-Barr virus (EBV), a ubiquitous B-lymphotropic herpesvirus, ectopically infects T or NK cells to cause severe diseases of unknown pathogenesis, including chronic active EBV infection (CAEBV) and EBV-associated hemophagocytic lymphohistiocytosis (EBV-HLH). We developed xenograft models CAEBV EBV-HLH by transplanting patients' PBMC immunodeficient mice the NOD/Shi-scid/IL-2Rγnull strain. In these models, EBV-infected T, NK, B proliferated systemically reproduced histological...
// Erika Onozawa 1, 2 , Haruna Shibayama Honami Takada Ken-Ichi Imadome 3 Sho Aoki Mayumi Yoshimori Norio Shimizu 4 Shigeyoshi Fujiwara 5, 6 Takatoshi Koyama Osamu Miura 1 and Ayako Arai Department of Hematology, Graduate School Medical Dental Sciences, Tokyo University (TMDU), Bunkyo-ku, Tokyo, Japan Laboratory Molecular Genetics Advanced Medicine for Infections, National Center Child Health Development (NCCHD), Setagaya-ku, Stem cell Regenerative Medicine, Institute Research, 5 Allergy...
To clarify the mechanism for development of Epstein-Barr virus (EBV)-positive T- or NK-cell neoplasms, we focused on costimulatory receptor CD137. We detected high expression CD137 gene and its protein EBV-positive lines as compared with EBV-negative cell lines. cells from lymphoproliferative disorders (EBV-T/NK-LPDs) patients also had significantly higher than control healthy donors. In presence IL-2, whose concentration in serum EBV-T/NK-LPDs was that donors, upregulated patients' whereas...
Although human term placenta-derived primary cytotrophoblasts (pCTBs) represent a good syncytiotrophoblast (STB) model, in vitro culture of pCTBs is not always easily accomplished. Y-27632, specific inhibitor Rho-associated coiled-coil containing kinases (ROCK), reportedly prevented apoptosis and improved cell-to-substrate adhesion stability dissociated cultured embryonic stem cells corneal endothelial cells. The Rho kinase pathway regulates various kinds cell behavior, some which are...
Congenital human cytomegalovirus (HCMV) infection, a leading cause of birth defects, is most often manifested as neurological disorders. The pathogenesis HCMV-induced disorders is, however, largely unresolved, primarily because limited availability model systems to analyze the effects HCMV infection on neural cells. An induced pluripotent stem cell (iPSC) line was established from fibroblast MRC5 by introducing Yamanaka’s four factors and then differentiate into stem/progenitor cells (NSPCs)...
Abstract Epstein–Barr virus‐positive T‐cell lymphoproliferative diseases ( EBV ‐T‐ LPD s) are rare lymphomas with poor prognosis. Although chemotherapeutic strategies such as CHOP have been often selected, they exhibited only limited efficacy. To clarify the mechanism of chemoresistance, we examined P‐glycoprotein (P‐gp) expression. P‐gp acts an energy‐dependent efflux pump that excretes drugs from cytoplasm, resulting in low‐intracellular drug concentrations and sensitivity to chemotherapy....
Epstein–Barr virus (EBV) is the primary cause of infectious mononucleosis and strongly implicated in etiology multiple lymphoid epithelial cancers. EBV core fusion machinery envelope proteins gH/gL gB coordinately mediate entry into its target cells, B lymphocytes suggesting these could induce antibodies that prevent infection. We previously reported immunization rabbits with recombinant or trimeric each induced markedly higher serum EBV-neutralizing titers for than leading vaccine candidate...
Epstein–Barr virus (EBV), implicated in numerous human diseases, including lymphoid malignancies, persistently infects peripheral B cells and transforms them into lymphoblastoid cell lines. Here we found that EBV equally infected from patients with X-linked hyper IgM syndrome those healthy donors; however, it hardly transformed cells, because of the dysfunctional gene CD40 ligand (CD40L) patients. Unlike CD40, CD40L is not usually expressed on cells. However, infection normal induced...
Imadome K‐I, Fukuda A, Kawano F, Imai Y, Ichikawa S, Mochizuki M, Shigeta T, Kakiuchi Sakamoto Kasahara Fujiwara S. Effective control of Epstein–Barr virus infection following pediatric liver transplantation by monitoring viral DNA load and lymphocyte surface markers. Abstract: EBV‐associated PTLD is a serious complication transplantation. We performed periodical molecular EBV in 140 consecutive patients who had living‐related the National Center for Child Health Development, Tokyo....
Argonaute (Ago) proteins function in RNA silencing as components of the RNA-induced complex (RISC). In lower organisms, small interfering and miRNA pathways diverge due part to sorting mechanisms that direct distinct (sRNA) duplexes onto specific Ago-RISCs. However, such appear be lost mammals. miRNAs not distinguish among Ago1–4. To determine effect viral infection on system, we compared content deep-sequenced extracted from immunoprecipitation experiments with Ago1 Ago2 using Epstein–Barr...
Abstract Background X-linked lymphoproliferative syndrome (XLP) is a rare inherited immunodeficiency by an extreme vulnerability to Epstein-Barr virus (EBV) infection, frequently resulting in hemophagocytic lymphohistiocytosis (HLH). XLP are now divided into type 1 (XLP-1) and 2 (XLP-2), which caused mutations of SH2D1A/SLAM-associated protein (SAP) inhibitor apoptosis (XIAP) genes, respectively. The diagnosis individuals with EBV-associated HLH (EBV-HLH) generally difficult because they...