A5065970835- Ion Channels and Receptors
- Neurobiology and Insect Physiology Research
- Postharvest Quality and Shelf Life Management
- Neuroscience and Neural Engineering
- Thermoregulation and physiological responses
- Biochemical Analysis and Sensing Techniques
- Ion channel regulation and function
- Plant Stress Responses and Tolerance
- Planarian Biology and Electrostimulation
- Nicotinic Acetylcholine Receptors Study
- Connexins and lens biology
- Phytochemicals and Antioxidant Activities
- Toxin Mechanisms and Immunotoxins
- Transcranial Magnetic Stimulation Studies
Czech Academy of Sciences
2017-2024
Czech Academy of Sciences, Institute of Physiology
2017-2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2021-2024
Charles University
2019-2020
The odontoblast, with processes beyond the dentin-enamel boundary, is initial site of TRPC5 cold transduction in teeth.
Transient receptor potential ankyrin 1 channel (TRPA1) serves as a key sensor for reactive electrophilic compounds across all species. Its sensitivity to temperature, however, differs among species, variability that has been attributed an evolutionary divergence. Mouse TRPA1 was implicated in noxious cold detection but later also identified one of the prime heat sensors. Moreover, human TRPA1, originally considered be temperature-insensitive, turned out act intrinsic bidirectional...
A cavity in the ion channel TRPA1 coordinates electrophilic, voltage, and calcium sensitivity might be targeted to treat pain other disorders.
Abstract TRP channels sense temperatures ranging from noxious cold to heat. Whether specialized thermosensor modules exist and how they control channel pore gating is unknown. We studied purified human TRPA1 (hTRPA1) truncated proteins gain insight into the temperature of hTRPA1. In patch-clamp bilayer recordings, ∆1–688 hTRPA1, without N-terminal ankyrin repeat domain (N-ARD), was more sensitive heat, whereas ∆1–854 also lacking S1–S4 voltage sensing-like (VSLD), gained sensitivity but lost...
Abstract Exposure to repetitive low‐frequency electromagnetic field ( LF ‐ EMF ) shows promise as a non‐invasive approach treat various sensory and neurological disorders. Despite considerable progress in the development of modern stimulation devices, there is limited understanding mechanisms underlying their biological effects potential targets at cellular level. A significant impact on voltage‐gated calcium channels downstream signalling pathways has been convincingly demonstrated many...
Our understanding of the general principles polymodal regulation transient receptor potential (TRP) ion channels has grown impressively in recent years as a result intense efforts protein structure determination by cryo-electron microscopy. In particular, high-resolution structures various TRP captured different conformations, number them determined membrane-mimetic environment, have yielded valuable insights into their architecture, gating properties and sites interactions with annular...
The transient receptor potential ankyrin 1 (TRPA1) channel is a polymodal sensor of environmental irritant compounds, endogenous proalgesic agents, and cold. Upon activation, TRPA1 channels increase cellular calcium levels via direct permeation trigger signaling pathways that hydrolyze phosphatidylinositol-4,5-bisphosphate (PIP2 ) in the inner membrane leaflet. Our objective was to determine extent which putative PIP2 -interaction site (Y1006-Q1031) involved regulation. interactions two...
The Transient Receptor Potential Ankyrin 1 (TRPA1) channel is an integrative molecular sensor for detecting environmental irritant compounds, endogenous proalgesic and inflammatory agents, pressure, temperature. Different post-translational modifications participate in the discrimination of essential functions TRPA1 its physiological environment, but underlying structural bases are poorly understood. Here, we explored role cytosolic N-terminal residue Ser602 located near a functionally...
Exposure to repetitive low‐frequency electromagnetic field (LF‐EMF) shows promise as a non‐invasive approach treat various sensory and neurological disorders. Despite considerable progress in the development of modern stimulation devices, there is limited understanding mechanisms underlying their biological effects potential targets at cellular level. A significant impact on voltage‐gated calcium channels downstream signaling pathways has been convincingly demonstrated many distinct cell...
Abstract The human TRPA1 (hTRPA1) is an intrinsic thermosensitive ion channel responding to both cold and heat, depending on the redox environment. Here, we have studied purified hTRPA1 truncated proteins gain further insight into temperature gating of hTRPA1. We found in patch-clamp bilayer recordings that Δ1-688 hTRPA1, without N-terminal ankyrin repeat domain (N-ARD), was more sensitive whereas Δ1-854 also lacking S1-S4 voltage sensing-like (VSLD) gained sensitivity but lost its heat...