A5073888616- Virus-based gene therapy research
- Veterinary Oncology Research
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Telemedicine and Telehealth Implementation
- Monoclonal and Polyclonal Antibodies Research
- Circular RNAs in diseases
- Hedgehog Signaling Pathway Studies
- 14-3-3 protein interactions
- Fungal Plant Pathogen Control
- Infectious Diseases and Mycology
- Chromatography in Natural Products
- Advanced Drug Delivery Systems
- Ethnobotanical and Medicinal Plants Studies
- Neuropeptides and Animal Physiology
- Phytochemistry and biological activities of Ficus species
Auburn University
2015-2024
Women's College Hospital
2023
University of Toronto
2023
Mahatma Gandhi Dental College & Hospital
2016
Notre Dame of Maryland University
2014
Govind Ballabh Pant University of Agriculture and Technology
2010
St. John's University
2008
MicroRNAs (miRNAs) are single-stranded, noncoding RNA molecules that regulate gene expression post-transcriptionally by binding to messenger RNAs. miRNAs important regulators of expression, and their dysregulation is implicated in many human dog diseases. Most cancers tested, date, have been shown express altered miRNA levels, which indicates potential importance the oncogenic process. Based on this evidence, numerous suggested as cancer biomarkers for both diagnosis prognosis. miRNA-based...
Abstract First-generation 4-1BB (CD137) monoclonal antibody (mAb)-based agonists demonstrated significant potential in early preclinical and clinical studies by eliciting robust anti-tumor responses. However, their development was hampered dose-limiting hepatotoxicity, attributed to the systemic activation of receptors on monocytes, which turn recruited CD8+ T cells attack hepatocytes. To address this challenge, next-generation therapies have focused decoupling hepatotoxic effects from its...
Abstract Characterized as a highly aggressive and metastatic solid bone tumor, osteosarcoma (OS) remains difficult to treat cancer. Even with the current treatment options of chemotherapy, radiation, tumor removal surgery there is poor patient prognosis low survival rates, highlighting need for novel treatments. Over last decade, chimeric antigen receptor (CAR) T cells have shown promising results in certain leukemias lymphomas are further being explored types. We aim develop CAR against...
Abstract Osteosarcoma (OS) is a mesenchymal neoplasm. OS makes up approximately 3% of all malignancies in children. The mortality rate has not changed the last few decades; therefore, development advanced cancer therapies essential. We hypothesize that next-generation conditionally replicative oncolytic virus with an armed anti-PD1 HcAb will lyse cells and initiate immune response against tumor microenvironment.The purpose study to evaluate activity production/ secretion Hcab by Canine...
Abstract p16/INK4A/CDKN2A is an important tumor suppressor gene that arrests cell cycle in G1 phase inhibiting binding of CDK4/6 with cyclin D1, leaving the Rb protein unphosphorylated and E2F bound inactive. We hypothesized p16 has a role exit from becomes defective cancer cells. Well characterized p16‐defective canine mammary lines (CMT28, CMT27, CMT12), derived stably p16‐transfected CMT clones (CMT27A, CMT27H, CMT28A, CMT28F), normal fibroblasts (NCF), were used to investigate expression...
Tulsi is a popular healing herb in Ayurvedic medicine. It widely used the treatment of several systemic diseases because its anti-microbial property. However, studies documenting effect on oral disease causing organisms are rare. Hence, an attempt was made to determine periodontal microorganism human dental plaque.To if Ocimum sanctum (Linn.) has activity (Minimum Inhibitory Concentration and zone inhibition) against Actinobacillus actinomycetemcomitans plaque compare antimicrobial...
Abstract Cancer is the leading cause of death in geriatric dog population. Currently, use immune checkpoint inhibitors (ICIs) such as anti-CTLA4 antibodies has markedly improved prognosis several cancers their advanced stages. However, ICIs targeting CTLA4 blockade to treat canine cancer patients are yet define. In this study, we sought develop, characterize and assess whether chimeric heavy chain only (cHcAbs) against viable therapeutic candidates for treatment cancers. Anti-CTLA4...
Abstract Mammary cancer is among the most frequently observed canine tumors in unspayed female dogs resulting death due to metastatic disease. These are excellent models of human breast but until recently there was only anecdotal evidence regarding underlying genetic defects. We reported expression defects cyclin‐dependent kinase p21/Cip1 and p53 three independent mammary tumor (CMT) cell lines derived from spontaneous cancers. investigated further same focusing on additional suppressor gene...
Well characterized, stable, p16-defective canine mammary cancer (CMT) cell lines and normal epithelial cells were used to investigate expression of the major breast cancer-specific hormone receptors estrogen receptor alpha (ER1) progesterone (PR) as well luminal epithelial-specific proto-oncogenes encoding c-
Delay in cancer diagnosis often results metastasis and an inability to successfully treat the tumor. The use of broadly cancer-specific biomarkers at early stage may improve treatment staging. This study has explored circulatory exosomal miRNAs as potential diagnostic identify patients. Secretory were isolated from 13 canine cell lines (lymphoma, mast tumor, histiocytic line, osteosarcoma, melanoma, breast tumor) sequenced by Next-Generation sequencing (NGS). We have identified 6 (cfa-miR-9,...
Abstract The INK4 family of cyclin‐dependent kinase inhibitors (CKI) encode important cell cycle regulators that tightly control during G1 to S phase. These related genes are considered tumor suppressors as loss function contributes the malignant phenotype. Expression CKIs p16, p14ARF, or p15 were defective in six different canine mammary (CMT) lines compared normal thoracic fibroblasts. This suggests CKI defects frequently responsible for neoplastic transformation carcinomas. p16 and p14ARF...
Adenoviruses are the most widely used vectors in cancer gene therapy. well characterized and easily manipulated. Adenovirus serotype 5 (Ad5) is commonly human serotype. Ad5 internalization into host cells a combined effect of binding fiber knob with coxsackie virus adenovirus receptor (CAR) RGD motifs viral penton to cell surface integrins (αvβ3, αvβ5). Ad5's wide range host-cell transduction lack integration genome have made it an excellent choice for therapeutics. However, has limited...
Recent breakthroughs in cancer immunotherapy have provided unprecedented clinical benefits to human patients. Cancer is also one of the most common causes death pet dogs. Thus, canine-specific immune therapies targeting similar signaling pathways can provide better treatment options for canine Here, we describe development and characterization two anti-OX40 agonists activate OX40 signaling. We show that OX40, like not expressed on resting T cells, its expression markedly increased CD4 cells...
Despite significant advances in cancer diagnosis and treatment, osteosarcoma (OSA), an aggressive primary bone tumor, has eluded attempts at improving patient survival for many decades. The difficulty managing OSA lies its extreme genetic complexity, drug resistance, heterogeneity, making it improbable that a single-target treatment would be beneficial the majority of affected individuals. Precision medicine seeks to fill this gap by addressing intra- inter-tumoral heterogeneity improve...
Extracting sufficient quantity and quality RNA from bone is essential for downstream application, such as transcriptomic sequencing, to evaluate gene expression. Isolation of presents a unique challenge owing the hypocellular, brittle mineralized matrix, which makes homogenizing tissue difficult provides little work with. Removal contaminating tissue, marrow connective isolating that osteoblasts, osteoclasts osteocytes. This study established method effectively isolate normal canine cells...