A5018243333- Renal Diseases and Glomerulopathies
- Cellular Mechanics and Interactions
- Characterization and Applications of Magnetic Nanoparticles
- Electronic and Structural Properties of Oxides
- Magnetic and Electromagnetic Effects
- Micro and Nano Robotics
- Chronic Kidney Disease and Diabetes
- Advanced Fluorescence Microscopy Techniques
- Dialysis and Renal Disease Management
- Multiferroics and related materials
- Microtubule and mitosis dynamics
- Microfluidic and Bio-sensing Technologies
- Renal and related cancers
- Ferroelectric and Piezoelectric Materials
- Cell Adhesion Molecules Research
- Wound Healing and Treatments
- Platelet Disorders and Treatments
- Geophysical and Geoelectrical Methods
- Genetic and Kidney Cyst Diseases
- Skin and Cellular Biology Research
- Single-cell and spatial transcriptomics
- Tendon Structure and Treatment
Washington University in St. Louis
2021-2025
University of California, Irvine
2023
U.S. National Science Foundation
2021
Mechanical stretch can activate long-lived changes in fibroblasts, increasing their contractility and initiating phenotypic transformations. This activation, critical to wound healing procedures such as skin grafting, increases with mechanical stimulus for cells cultured two-dimensional but is highly variable three-dimensional (3D) tissue. Here, we show that static of 3D tissues either increase or decrease fibroblast activation depending upon recursive cell–extracellular matrix (ECM)...
Significance Statement Podocytes have interdigitated foot processes with intricate three-dimensional structures that are crucial for glomerular filtration. Electron microscopy shows podocytes’ complex morphology, but direct visualization of their cytoskeleton and definitive identification the proteins comprise cytoskeletal remained elusive. The authors describe a novel technique reveals organization podocyte cytoskeleton, finding actin cables inside connected directly to slit diaphragms,...
Chronic kidney diseases are widespread and incurable. The biophysical mechanisms underlying them unclear, in part because material systems for reconstituting the microenvironment of relevant cells limited. A critical question is how podocytes (glomerular epithelial cells) regenerate foot processes filtration apparatus following injury. Recently identified sarcomere-like structures (SLSs) with periodically spaced myosin IIA synaptopodin appear injured vivo. We hypothesized that SLSs template...
In ferroelectric heterostructures, the interaction between intrinsic polarization and electric field generates a rich set of localized electrical properties. The local is determined by several connected factors, including charge distribution individual unit cells, interfacial electromechanical boundary conditions, chemical composition interfaces. However, especially in perovskites, complete description across micro-, nano-, atomic-length scales missing. Here, applying four-dimensional...
Abstract Mechanical stretching of living tissues can activate long-lived changes in tissue cells such as fibroblasts, increasing their contractility and initiating phenotypic transformations. Increased mechanical stimulus typically leads to monotonically activation fibroblasts cultured 2D, but levels are difficult predict for 3D fibrous tissues, leading variable outcomes procedures skin grafting. Here we report that the source this variation is cell-extracellular matrix (ECM) interactions...
Qu, Chengqing; Jiang, Shumeng; Genin, Guy M.; Suleiman, Hani; Miner, Jeffrey H. Author Information
Abstract Although actin stress fibers are abundant in cultured cells, little is known about these structures vivo. In podocytes of the kidney glomerulus, much evidence suggests that mechanobiological mechanisms underlie injury, with changes to fiber potentially responsible for pathological cell morphology. However, this hypothesis difficult rigorously test vivo due challenges visualization. We therefore developed first visualization technique capable resolving three-dimensional (3D) podocyte...