A5061564124- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Nanopore and Nanochannel Transport Studies
- Cancer Genomics and Diagnostics
- Electrostatics and Colloid Interactions
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Advanced Electron Microscopy Techniques and Applications
- Epigenetics and DNA Methylation
- Renal cell carcinoma treatment
- Molecular Communication and Nanonetworks
- Retinal Development and Disorders
- Genetic factors in colorectal cancer
- Advanced Biosensing Techniques and Applications
- Risk and Safety Analysis
- Reliability and Maintenance Optimization
- Genomic variations and chromosomal abnormalities
- Molecular Biology Techniques and Applications
- Photochromic and Fluorescence Chemistry
- Orbital Angular Momentum in Optics
- Lipid Membrane Structure and Behavior
Grail (United States)
2020
Menlo School
2020
University of Pittsburgh
2012
Harvard University
2009-2011
Harvard University Press
2009-2011
Early cancer detection could identify tumors at a time when outcomes are superior and treatment is less morbid. This prospective case-control sub-study (from NCT02889978 NCT03085888) assessed the performance of targeted methylation analysis circulating cell-free DNA (cfDNA) to detect localize multiple types across all stages high specificity.
Anti-Brownian electrokinetic traps have been used to trap and study the free-solution dynamics of large protein complexes long chains DNA. Small molecules in solution thus far proved too mobile by any means. Here we explore ultimate limits on trapping single molecules. We developed a feedback-based anti-Brownian which classical thermal noise is compensated maximal extent allowed quantum measurement noise. trapped fluorophores with molecular weight < 1 kDa hydrodynamic radius 6.7 Å for...
Fluorescence imaging is used to study the dynamics of a wide variety single molecules in solution or attached surface. Two key challenges this pursuit are (1) image immobilized presence high level fluorescent background and (2) freely diffusing for long times. Strategies that perform well by one measure often poorly other. Here, we present simple modification wide-field fluorescence microscope addresses both dramatically improves single-molecule imaging. The technique convex lens-induced...
Optical tracking of a fluorescent particle in solution faces fundamental constraints due to Brownian motion, diffraction, and photon shot noise. Background photons imperfect apparatus further degrade precision. Here we use model motion combine information from multiple time-points improve the localization We derive successive approximations that enable real-time with well controlled tradeoffs between precision computational cost. present theory context feedback electrokinetic trapping,...
Abstract Microbial rhodopsins are an important class of light‐activated transmembrane proteins whose function is typically studied on bulk samples. Herein, we apply photochromic fluorescence resonance energy transfer to investigate the dynamics these with sensitivity approaching single‐molecule limit. The brightness a covalently linked organic fluorophore modulated by changes in absorption spectrum endogenous retinal chromophore that occur as molecule undergoes photocycle. We photocycles...
It has recently become possible to trap individual fluorescent biomolecules in aqueous solution by using real-time tracking and active feedback suppress Brownian motion. We propose areas of investigation which anti-Brownian electrokinetic (ABEL) trapping single molecules is likely lead significant new insights into biomolecular dynamics.
Abstract Single‐molecule spectroscopy provides a wealth of information on the dynamics and interactions complex biological molecules. Yet these measurements are extremely challenging, partly because Brownian motion prevents molecules in free solution from remaining stationary. Here we describe several techniques that our lab has developed for confining single optical spectroscopy. These alternatives to surface immobilization provide confinement is gentle enough minimize perturbations...
Abstract Background: Earlier cancer detection could identify tumors when they are more treatable and thus may improve clinical outcomes. A blood-based multicancer early test using circulating tumor cell-free DNA (cfDNA) offers the potential to address this unmet need. Our previous discovery work identified whole-genome bisulfite sequencing as outperforming targeted approaches for across stages. We developed a methylation (TM) assay tissue-of-origin (TOO) localization. Methods: Participants...