A5090226553- Sexual function and dysfunction studies
- Pelvic floor disorders treatments
- Hormonal and reproductive studies
- Menopause: Health Impacts and Treatments
- Urinary Bladder and Prostate Research
- Cancer Genomics and Diagnostics
- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Marriage and Sexual Relationships
- Sexual Differentiation and Disorders
- Sexuality, Behavior, and Technology
- CRISPR and Genetic Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Molecular Biology Techniques and Applications
- Reproductive Health and Contraception
- Sex and Gender in Healthcare
- Birth, Development, and Health
- Hormonal Regulation and Hypertension
- Ovarian function and disorders
- Genetic factors in colorectal cancer
- Genomic variations and chromosomal abnormalities
- Apelin-related biomedical research
- Female Genital Mutilation/Cutting Issues
- Epigenetics and DNA Methylation
- Adolescent Sexual and Reproductive Health
Illumina (United States)
2023
Berman Center for Outcomes and Clinical Research
1999-2021
Grail (United States)
2019-2020
Menlo School
2019-2020
Bio-Rad (United States)
2013-2016
Temple University
2014
University of California, San Francisco
2003-2010
Stanford University
2008-2009
Palo Alto University
2009
University of California, Los Angeles
2001-2006
Female sexual dysfunction is highly prevalent but not well defined or understood. We evaluated and revised existing definitions classifications of female dysfunction.An interdisciplinary consensus conference panel consisting 19 experts in selected from 5 countries was convened by the Sexual Function Health Council American Foundation for Urologic Disease. A modified Delphi method used to develop classifications, build on framework International Classification Diseases-10 DSM-IV: Diagnostic...
Early cancer detection could identify tumors at a time when outcomes are superior and treatment is less morbid. This prospective case-control sub-study (from NCT02889978 NCT03085888) assessed the performance of targeted methylation analysis circulating cell-free DNA (cfDNA) to detect localize multiple types across all stages high specificity.
Hematopoietic stem cells from patients with sickle cell disease can be edited by CRISPR/Cas9 and maintain the edits in vivo.
Abstract Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing rate high-fidelity edits. The identification conditions that favor HDR over NHEJ has been hindered lack a simple method to measure and directly simultaneously at endogenous loci. To overcome this challenge, we developed novel, rapid,...
We evaluated the efficacy and safety of sildenafil citrate in spontaneously or surgically postmenopausal women with female sexual arousal disorder (FSAD).Sildenafil (a 50 mg dose adjustable to 100 25 mg) was a 12-week, double-blind, placebo controlled study 202 FSAD who had protocol specified estradiol free testosterone concentrations, and/or were receiving estrogen androgen replacement therapy. Patients excluded if emotional, relationship historical abuse issues contributed significantly...
Two years ago, we described the first droplet digital PCR (ddPCR) system aimed at empowering all researchers with a tool that removes substantial uncertainties associated using analogue standard, quantitative real-time (qPCR). This enabled TaqMan hydrolysis probe-based assays for absolute quantification of nucleic acids. Due to significant advancements in chemistry and buoyed by multiple benefits dye-based target detection, have created "second generation" ddPCR compatible both TaqMan-probe...
The Endogenous Metabolite Glycerophosphocholine Promotes Longevity and Fitness in Caenorhabditis elegans,
Thirty-one patients completed a Genital Self-Image Scale along with the Female Sexual Function Index (FSFI; Rosen et al., 2000), Distress (Derogatis 2001), Perceived Stress (Cohen 1983), Golombok Rust Inventory of Marital State (Rust 1986) Questionnaire, and Beck's Depression (Beck 1988). A positive genital self-image correlated negatively amount sexual distress depression but not marital state, perceived stress, or overall function. was associated higher desire arousal, lubrication, orgasm,...
Abstract Sexual dysfunction is a complaint of 30-50% American women. Aside from hormone replacement therapy, there are no current FDA-approved medical treatments for female sexual complaints. The goal this pilot study was to determine safety and efficacy sildenafil use in women with arousal disorder (SAD). Evaluations were completed on 48 complaints SAD. Physiologic measurements, including genital blood flow, vaginal lubrication, intravaginal pressure-volume changes, sensation recorded pre-...
Purpose: Female sexual dysfunction is highly prevalent but not well defined or understood. We evaluated and revised existing definitions classifications of female dysfunction.Materials a...
In Caenorhabditis elegans and Drosophila melanogaster, removing the germline precursor cells increases lifespan. worms, possibly also in flies, this lifespan extension requires presence of somatic reproductive tissues. How gonad signals other tissues to increase is not known. The triggered by loss germ known require sterol hormone signaling, as reducing activity nuclear receptor DAF-12, or genes required for synthesis DAF-12 ligand dafachronic acid, prevents from extending addition FOXO...
Melanin-concentrating hormone (MCH) regulates feeding and complex behaviors in mammals pigmentation fish. The relationship between fish mammalian MCH systems is not well understood. Here, we identify characterize two genes zebrafish, Pmch1 Pmch2. Whereas its corresponding MCH1 peptide resemble found other fish, the zebrafish Pmch2 gene MCH2 share genomic structure, synteny, high sequence homology with MCH. Zebrafish Pmch are expressed closely associated but non-overlapping neurons within...
The rapid adoption of gene editing tools such as CRISPRs and TALENs for research eventually therapeutics necessitates assays that can rapidly detect quantitate the desired alterations. Currently, most commonly used assay employs "mismatch nucleases" T7E1 or "Surveyor" recognize cleave heteroduplexed DNA amplicons containing mismatched base-pairs. However, this is prone to false positives due cancer-associated mutations and/or SNPs requires large amounts starting material. Here we describe a...
Although Female Sexual Dysfunction (FSD) affects 40% of American women, there is no FDA-approved pharmaceutical therapy. The EROS-CTD (Clitoral Therapy Device, UroMetrics, Inc., St. Paul, MN) treatment the first FDA cleared-to-market therapy for FSD. Clitoral engorgement believed to play an important role in female sexual arousal and overall satisfaction. a small, battery-powered device designed enhance clitoral engorgement, increase blood flow clitoris, ultimately improve women with...