A5044370170- Connective Tissue Growth Factor Research
- Biomarkers in Disease Mechanisms
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Bone and Dental Protein Studies
- Cancer-related Molecular Pathways
- Protein Degradation and Inhibitors
- Retinoids in leukemia and cellular processes
- Cancer Cells and Metastasis
- Genetic Syndromes and Imprinting
- Wnt/β-catenin signaling in development and cancer
- Cancer Genomics and Diagnostics
- PARP inhibition in cancer therapy
- Cancer-related gene regulation
- Ion Transport and Channel Regulation
- Inflammatory mediators and NSAID effects
- Endoplasmic Reticulum Stress and Disease
- Genomic variations and chromosomal abnormalities
- Calcium Carbonate Crystallization and Inhibition
University of Alberta
2015-2024
Children's Hospital of Eastern Ontario
2019-2024
Agricultural Research Institute of Ontario
2023
University of Ottawa
2022
Washington Center
2022
Western University
2011-2020
Cancer stem-like cells (SLC) resist conventional therapies, necessitating searches for SLC-specific targets. We established that cyclo-oxygenase(COX)-2 expression promotes human breast cancer progression by activation of the prostaglandin(PG)E-2 receptor EP4. Present study revealed COX-2 induces SLCs EP4-mediated NOTCH/WNT signaling. Ectopic over-expression in MCF-7 and SKBR-3 cell lines resulted in: increased migration/invasion/proliferation, epithelial-mesenchymal transition (EMT),...
Abstract Plasticity of neoplasia, whereby cancer cells attain stem-cell-like properties, is required for disease progression and represents a major therapeutic challenge. We report that in breast NANOG , SNAIL NODAL transcripts manifest multiple isoforms characterized by different 5’ Untranslated Regions (5’UTRs), translation subset these stimulated under hypoxia. The accumulation the corresponding proteins induces plasticity “fate-switching” toward stem cell-like phenotypes....
Breast cancer researchers use cell lines to model myriad phenomena ranging from DNA repair stem phenotypes. Though appropriate, and even requisite, for many studies, the suitability of as tumour models has come into question owing possibilities tissue culture artefacts clonal selection. These issues are compounded by inability cells grown in isolation fully situ environment, which also contains a plethora non-tumour types. It is thus important understand similarities differences between...
The rapid adoption of gene editing tools such as CRISPRs and TALENs for research eventually therapeutics necessitates assays that can rapidly detect quantitate the desired alterations. Currently, most commonly used assay employs "mismatch nucleases" T7E1 or "Surveyor" recognize cleave heteroduplexed DNA amplicons containing mismatched base-pairs. However, this is prone to false positives due cancer-associated mutations and/or SNPs requires large amounts starting material. Here we describe a...
// Krista Marie Vincent 1, 2 , Lynne-Marie Postovit 1 Department of Oncology, Faculty Medicine and Dentistry, University Alberta, Edmonton, AB, T6G 2E1, Canada Anatomy Cell Biology, Western Ontario, London, ON N6A 3K7, Correspondence to: Postovit, email: postovit@ualberta.ca Keywords: cell lines, tumour models, microenvironment Received: October 13, 2016     Accepted: December Published: January 02, 2017 ABSTRACT Melanoma researchers utilize lines to model many phenomena....
Abstract We show that Cyclooxygenase-2 over-expression induces an oncogenic microRNA miR655 in human breast cancer cells by activation of EP4. MiR655 expression positively correlated with COX-2 genetically disparate cell lines and increased all when grown as spheroids, implicating its link stem-like (SLCs). Ectopic MCF7 SKBR3 resulted proliferation, migration, invasion, spheroid formation Epithelial to Masenchymal transition (EMT). Conversely, knocking down aggressive MCF7-COX2 SKBR3-COX2...
Melanoma is the leading cause of skin cancer-related death. As prognosis patients with melanoma remains problematic, identification new therapeutic targets essential. Matricellular proteins are nonstructural extracellular matrix proteins. They secreted into tumor microenvironment to coordinate behavior among different cell types, yet their contribution underinvestigated. Examples matricellular include those comprising CCN family. The family member, CCN1, highly proangiogenic. Herein, we show...
Myristoylation, the N-terminal modification of proteins with fatty acid myristate, is critical for membrane targeting and cell signaling. Because cancer cells often have increased N-myristoyltransferase (NMT) expression, NMTs were proposed as anti-cancer targets. To systematically investigate this, we performed robotic line screens discovered a marked sensitivity hematological lines, including B-cell lymphomas, to potent pan-NMT inhibitor PCLX-001. PCLX-001 treatment impacts global...
Terminal deletions of chromosome 14q are characterized by a spectrum phenotypes that can include microcephaly, growth deficiency, intellectual disability, characteristic facial features, and various congenital anomalies. The rarity this syndrome, together with the broad phenotypes, has made genotype-phenotype correlations difficult. Herein, we describe family core features condition heterozygous 3.7 Mb deletion at 14q32.32qter. To our knowledge, is first case vertical transmission terminal...
Abstract Secreted frizzled-related proteins (SFRPs), containing five family members (SFRPs 1–5) are putative extracellular Wnt inhibitors. Given their abilities to inhibit signalling, as well the loss of SFRP1 in many cancers, this is generally considered be tumour suppressive. In study we analyzed gene expression, promoter methylation and survival data from over 8000 normal samples 29 cancers order map context-specific associations SFRPs 1–5 with patient survival, silencing expression...
Research Article13 March 2017Open Access Transparent process Deletion of F4L (ribonucleotide reductase) in vaccinia virus produces a selective oncolytic and promotes anti-tumor immunity with superior safety bladder cancer models Kyle G Potts Department Oncology, Faculty Medicine Dentistry, University Alberta, Edmonton, AB, Canada Li Ka Shing Institute Virology, Cancer Northern Alberta (CRINA), Search for more papers by this author Chad R Irwin Medical Microbiology & Immunology, Nicole A...
Abstract Background In humans, two ubiquitously expressed N-myristoyltransferases, NMT1 and NMT2, catalyze myristate transfer to proteins facilitate membrane targeting signaling. We investigated the expression of NMT s in numerous cancers found that NMT2 levels are dysregulated by epigenetic suppression, particularly so hematologic malignancies. This suggests pharmacological inhibition remaining could allow for selective killing these cells, sparing normal cells with both NMTs. Methods...
Osteopontin (OPN) inhibits the nucleation and/or growth of several biominerals, including hydroxyapatite (HA) and calcium oxalate monohydrate (COM), is thought to function in prevention soft-tissue calcification. In previous studies, pOPAR, a peptide corresponding amino acids 65–80 rat bone OPN (pSHDHMDDDDDDDDDGD), was shown be potent inhibitor HA crystal growth. We now show that formation COM presence this results plate-shaped crystals with rounded ends scalloped {100} faces. Measurement...
Glioblastoma (GBM) is the most common and aggressive malignant primary brain tumor in adults. The standard treatment achieves a median overall survival for GBM patients of only 15 months. Hence, novel therapies based on an increased understanding mechanistic underpinnings are desperately needed. In this study, we show that elevated expression 28S rRNA (cytosine-C(5))-methyltransferase NSUN5, which methylates cytosine 3782 cells, strongly associated with poor patients. Moreover, demonstrate...
Epigenetic silencing of RASSF1A is frequently observed in numerous cancers and has been previously reported. The promoter region predicted to have 75 CpG sites, very few studies demonstrate how the methylation these sites affects expression. In addition, expression relationship between its downstream target, modulator apoptosis 1 (MOAP-1), poorly understood. this study, we explored mRNA RASSF1A, MOAP-1 well-characterized splice variant RASSF1, RASSF1C, cancer cell lines primary tumors. We...