A5013661088- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Hepatitis Viruses Studies and Epidemiology
- Andrographolide Research and Applications
- COVID-19 Clinical Research Studies
- Polyomavirus and related diseases
- SARS-CoV-2 detection and testing
- Bartonella species infections research
- Food Allergy and Anaphylaxis Research
- Viral-associated cancers and disorders
- PARP inhibition in cancer therapy
- Parvovirus B19 Infection Studies
- Poxvirus research and outbreaks
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Collaboration in agile enterprises
- Pharmacological Effects and Toxicity Studies
- Acne and Rosacea Treatments and Effects
- Extracellular vesicles in disease
- Virus-based gene therapy research
Fujita Health University
2013-2025
Fujita Health University Hospital
2016-2025
Kyoto Prefectural University of Medicine
2023
Japanese Red Cross Society Kyoto Daini Hospital
2023
Ritsumeikan University
2021
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated to diagnose several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 real-life clinical settings remains unclear. This study aimed determine the sensitivity and specificity of RT-LAMP compared reverse transcription-quantitative polymerase chain reaction (RT-qPCR) over disease course COVID-19.
In developing countries, acute gastroenteritis (AGE) is a leading cause of death in children younger than 5 years. Myanmar, no comprehensive study has been done to investigate the microorganisms responsible for AGE among hospitalized children. Multiplex polymerase chain reaction (PCR) was used identify Myanmar before introduction rotavirus vaccine. This prospective enrolled 12 years with who were at Yankin Children's Hospital Yangon, between September 2019 and February 2020. PCR (FilmArrayTM...
The loop-mediated isothermal amplification (LAMP) method was developed to distinguish between the varicella-zoster virus (VZV) vaccine (vOka) strain and wild-type strains. Two single nucleotide polymorphisms (SNPs) (nucleotide [nt] 105705 for VR-1 VZV LAMP nt 106262 VR-2 LAMP) located in open reading frame 62 gene were selected as targets. Amplified vOka DNA demonstrated a typical ladder pattern; however, no product detected reactions performed with DNAs from other human herpesviruses by...
Approximately 1 percent of healthy individuals carry human herpesvirus-6 within a host chromosome. This is referred to as chromosomally integrated (CIHHV-6). In this study, we investigated the chromosomal integration site in six harboring CIHHV-6B. Using FISH, found that HHV-6B signals are consistently located at telomeric region. The proximal endpoints virus were mapped one two telomere-repeat-like sequences (TRSs) DR-R all cases. cases, isolated junction fragments between viral TRS and...
ABSTRACT In order to assess the full spectrum of human herpesvirus 6A (HHV-6A)- and HHV-6B-associated diseases, we sought develop an HHV-6 species-specific serological assay based on immunoblot analysis. The immunodominant proteins encoded by open reading frame U11, p100 for HHV-6A (strain U1102) 101K HHV-6B Z29), were selected generate virus antigens. Recombinant produced in a prokaryotic expression system. these was confirmed using anti-His tag 101K-specific monoclonal antibodies....
We analyzed serially collected serum samples from healthy adults who underwent BNT162b2 vaccination to elucidate the association between spike (S)-IgG antibody titers determined by ELISA using WHO international standard (NIBSC code 20/136) and neutralizing against three live SARS-CoV-2 variants. This study included 53 health care workers received two doses of vaccine. S-IgG nucleocapsid (N)-IgG were measured ELISA. Neutralizing (NT) responses variants (Wuhan D614 G: KUH003, Alpha, Delta)...
To establish a point-of-care test for coronavirus disease 2019 (COVID-19), we developed dry loop-mediated isothermal amplification (LAMP) method to detect severe acute respiratory syndrome 2 (SARS-CoV-2) RNA.We carried out reverse transcription (RT)-LAMP using the Loopamp SARS-CoV-2 Detection kit (Eiken Chemical, Tokyo, Japan). The entire mixture, except primers, is dried and immobilized inside tube lid.To determine specificity of kit, 22 viruses associated with infections, including...
Rotavirus (RV) is a leading cause of gastroenteritis in children. In Japan, Rotarix (RV1; GlaxoSmithKline), which monovalent vaccine derived from human RV (G1P[8]), has been introduced since November 2011, and RotaTeq (RV5; MSD) an pentavalent, human-bovine mono-reassortant (G1, G2, G3, G4, P1A[8]), July 2012. Long-term follow-up on efficacy genotypical change should be carried out order to control infection. The (RVGE) outbreak occurred during the 2018/2019 season Aichi prefecture, Japan....
We encountered a previously healthy 3-year-old girl with interstitial pneumonitis that initially developed due to human adenovirus type 2 infection and exacerbated by primary herpesvirus 7 infection. A comprehensive serum biomarker analysis showed patterns differed viral infection, suggesting respiratory lymphotropic infections might have different pathophysiology in pneumonitis.
The live attenuated human rotavirus vaccine strain RIX4414 (Rotarix®) is used worldwide to prevent severe rotavirus-induced diarrhea in infants. This was through the cell culture passaging of its predecessor, 89-12, which resulted multiple genomic mutations. However, specific molecular reasons underlying attenuation have remained elusive, primarily due absence a suitable reverse genetics system enabling precise genetic manipulations. Therefore, we first completed sequencing genome and then...
Abstract In the era of universal varicella vaccination, diagnosis is challenging, especially for breakthrough cases. We sought to clarify reliability direct varicella‐zoster virus (VZV) loop‐mediated isothermal amplification (LAMP) and DermaQuick® VZV using immunochromatography technique as rapid diagnostic tests varicella. addition, usefulness saliva a sample type LAMP was investigated. Among 46 enrolled patients with suspected infection, 31 (67.3%) were positive nucleic acid test based on...
RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it unclear how each strain replicates in intestinal tissue and excreted stool. To examine this issue, we established RV5 genotype-specific real-time reverse transcription-PCR (RT-PCR) assays. Five RT-PCR assays were designed the VP7 gene genotypes G1, G2, G3, G4, G6. All exhibited excellent linearity, detection limit was 1 infectious...
Abstract Objective The main aims of the present study were to elucidate systemic group A rotavirus (RVA) infection and clarify genetic changes persistent virus in X‐linked severe combined immunodeficiency (SCID) patient. Methods RotaTeq vaccine (RV5) genotype‐specific real‐time reverse transcription polymerase chain reaction was used monitor viral RNA load serially collected serum stool samples. Next‐generation sequence analysis determine genotype by sequencing 11 gene segments....
Human herpesvirus 6 (HHV‐6) is classified as two distinct species: HHV‐6A and B. HHV‐6B infection can cause several clinical manifestations in transplant recipients including encephalitis, bone marrow suppression, pneumonitis. In contrast to HHV‐6B, the features of remain largely undefined. Herein, we developed a multiplex cycling probe real‐time PCR that discriminated between HHV‐6B. The assay was HHV‐6‐specific no cross amplification demonstrated for other herpesviruses. Moreover, had...
The group A rotavirus (RVA) genome comprising 11 double-stranded RNAs encodes six structural proteins (VP1-VP4, VP6, and VP7) non-structural (NSP1-NSP6). Among these 12 rotaviral proteins, NSP6 has been less studied as to its function. We previously prepared a recombinant NSP6-deficient RVA derived from simian strain SA11-L2 by reverse genetics, found that the virus grew well in cell culture, although growth was abundant than of parental strain. In this study, we examined potency incapable...
Abstract Nonpharmaceutical interventions (NPIs) to control COVID‐19 have decreased the incidence of many pediatric infectious diseases. The epidemiology β‐ and γ‐herpesvirus infections might been affected by NPIs. aim this study was elucidate changes in trends complex febrile seizures (cFS) viral etiology before during pandemic. Between April 2017 March 2021, children aged ≤5 years were enrolled. Detection EBV, CMV, HHV‐6B, HHV‐7 DNA serum performed using real‐time PCR. cFS compared between...
In order to determine whether mixed infections of human herpesvirus 6B (HHV-6B) occur in immunocompetent and immunocompromised individuals, we examined the copy numbers telomeric repeat sequences (TRS) clinical isolates. isolates obtained from patients with exanthem subitum caused by primary HHV-6B infection, PCR products TRS ranging between 400 800 bp were amplified. various sizes amplified four drug-induced hypersensitivity syndrome (DIHS) 15 hematopoietic stem cell transplant (HSCT)...
To elucidate the seroprevalence and rate of asymptomatic infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Japanese children, serological analysis was performed using serum samples collected from March 2020 to February 2023. A total 1493 were during first study period (March 2021). None positive for SARS-CoV-2 antibody. In second 2021 2022), seven 1055 patients (0.7%) experienced infection. The third 2022 2023) divided into three terms: June 30, 2022; July...