A5072270931- Sphingolipid Metabolism and Signaling
- Inflammatory mediators and NSAID effects
- Retinal Development and Disorders
- Eicosanoids and Hypertension Pharmacology
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and biosynthesis
- ATP Synthase and ATPases Research
- Retinopathy of Prematurity Studies
- Neonatal and fetal brain pathology
- Genetic Syndromes and Imprinting
- Visual perception and processing mechanisms
- Receptor Mechanisms and Signaling
- Atherosclerosis and Cardiovascular Diseases
- Cholesterol and Lipid Metabolism
- Milk Quality and Mastitis in Dairy Cows
- Adipokines, Inflammation, and Metabolic Diseases
- Paraoxonase enzyme and polymorphisms
- melanin and skin pigmentation
- Salmonella and Campylobacter epidemiology
- EEG and Brain-Computer Interfaces
- Bacterial Identification and Susceptibility Testing
- Metabolomics and Mass Spectrometry Studies
- Ophthalmology and Visual Impairment Studies
- Regulation of Appetite and Obesity
- Neural dynamics and brain function
Lexington VA Health Care System
2017-2023
University of Kentucky
2017-2022
Vanderbilt University Medical Center
2016-2019
University of Gothenburg
1998-2017
Vanderbilt University
2015-2016
Heinrich Heine University Düsseldorf
2015
Deutsches Diabetes-Zentrum e.V.
2015
German Institute of Human Nutrition
2015
German Center for Diabetes Research
2015
University of Alabama in Huntsville
2015
Autotaxin (ATX) is a secreted enzyme that generates the bioactive lipid lysophosphatidic acid (LPA). We generated mice with global inducible post-natal inactivation or adipose-specific loss of Enpp2 gene encoding ATX. The animals are phenotypically unremarkable and exhibit differences in adipocyte size adipose tissue expression inflammatory genes after high fat feeding without gross distribution body mass. Surprisingly, both models Enpp2- deficiency exhibited marked protection from...
Elevated circulating levels of ceramides (Cers) are associated with increased risk cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality result coronary artery disease. Although the mechanisms involved likely complex, these associations suggest lowering Cer could be protective against cardiovascular diseases. Conversely, dietary fibers, inulin, have been reported to promote health. However, processes also not...
The widely expressed lysophosphatidic acid (LPA) selective receptor 4 (LPAR4) contributes to vascular development in mice and zebrafish. LPAR4 regulates endothelial permeability, lymphocyte migration, hematopoiesis, which could contribute atherosclerosis. We investigated the role of experimental atherosclerosis elicited by adeno-associated virus expressing PCSK9 lower LDL levels. After 20 weeks on a Western diet, cholesterol levels lipoprotein distribution were similar WT male
Lysophosphatidic acids (LPAs) are bioactive radyl hydrocarbon-substituted derivatives of glycerol 3-phosphate. LPA metabolism and signaling implicated in heritable risk coronary artery disease. Genetic pharmacological inhibition these processes attenuate experimental atherosclerosis. accumulates atheromas, which may be a consequence association with LDLs. The source, regulation, biological activity LDL-associated unknown. We examined the effects hyperlipidemia on levels distribution...
Here we sought to define behavioural traits linked anxiety, reward, and exploration in different strains of rats commonly used obesity research. We hypothesized that genetic variance may contribute not only their metabolic phenotype (that is well documented) but also the expression these traits. Rat differ susceptibility develop an obese (Sprague-Dawley, Obese Prone, Resistant, Zucker rats) were exposed a number tests starting at age 8 weeks. found similar susceptible models, Prone rats,...
In humans and rodents, risk of metabolic syndrome is sexually dimorphic, with an increased incidence in males. Additionally, the protective role female gonadal hormones ostensible, as prevalence type 2 diabetes mellitus (T2DM) increases after menopause. Here, we investigated influence estrogen (E2) on onset T2DM New Zealand obese (NZO) mice. Diabetes (defined blood glucose levels >16.6 mmol/l) NZO females high-fat diet (60 kcal% fat) week 22 was 43%. This markedly dependent liver fat content...
<h3>Aim</h3> To evaluate ophthalmological findings in children with Silver–Russell syndrome (SRS). <h3>Methods</h3> An evaluation including visual acuity (VA), refraction, strabismus, near point of convergence (NPC), slit-lamp examination, ophthalmoscopy, axial length measurements and full-field electroretinogram was performed on 18 SRS (8 girls, 10 boys; mean age 11.6 years). Fundus photographs were taken for digital image analysis. Data compared data an age- gender-matched reference group...
Abstract Genetic variants that regulate lipid phosphate phosphatase 3 (LPP3) expression are risk factors for the development of atherosclerotic cardiovascular disease. LPP3 is dynamically upregulated in context vascular inflammation with particularly heightened smooth muscle cells (SMC), however, impact on pathology not fully understood. We investigated role and lysophospholipid signaling a well-defined model pathologic aortic injury observed Angiotensin II (Ang II) increases PLPP3 SMCs...
Montanoa guatemalensis is a small to medium-sized tree in the Asteraceae that grows Central America from Mexico south through Costa Rica. There have been no previous investigations on essential oil of this tree.The leaf oils M. were obtained different individual trees growing Monteverde, Rica, two years, and analyzed by gas chromatography-mass spectrometry.The 2008 rich sesquiterpenoids, dominated α-selinene, β-selinene, cyclocolorenone, with lesser amounts monoterpenes α-pinene limonene. In...
A dry-film coliform count plate that is inoculated with 5 mL sample was compared the Violet Red Bile Agar method in a collaborative study by 18 laboratories. Products analyzed were 2% milk, chocolate cream, vanilla ice cottage cheese, and cheese. Collaborators tested blind duplicate uninoculated samples at low, medium, high level. Significantly (P < 0.05) higher numbers of coliforms recovered from milk 3 inoculum levels, low- high-inoculum cream counts obtained agar for cheese low-inoculum...
Prostaglandins are key modulators of blood pressure and arterial tone. Prostaglandin E 2 (PGE ), is a prostanoid that has vasodepressor effects; however, under certain circumstances PGE can induce vasopressor responses. Recent reports demonstrated sub-threshold concentrations vasoconstrictors augment -mediated constriction in rat femoral arteries. However, whether angiotensin II (Ang II) could affect contraction not known. Using wire myograph, we had no significant effect on mouse rings at...
We recently identified a pathway underlying immune activation in hypertension. Proteins oxidatively modified by reactive γ-ketoaldehydes (isoketals) accumulate dendritic cells (DCs). These are immunogenic and lead to subsequent T lymphocytes activation. The local signals that stimulate DCs isoketal adducts remain undefined. Prostaglandin E 2 (PGE ) has been implicated the inflammation associated with hypothesized PGE via its EP3 receptor contributes DC -/- mice wild type (WT) littermates...
We recently identified a pathway that underlies immune activation in hypertension. Proteins are oxidatively modified by highly reactive γ‐ketoaldehydes (isoketals) accumulate dendritic cells (DCs), and these isoketal‐adducted proteins immunogenic lead to subsequent of T lymphocytes. The local signals stimulate DCs isoketal adducted remains undefined. Mediators such as angiotensin II, norepinephrine prostaglandin E 2 (PGE ) have been implicated the inflammation associated with hypertension...
Normally eye contact and responsive smile in infants develop within 3 to 8 weeks postnatally. Delays may be due ocular/eye diseases, neurological conditions or have unknown etiology. The flash visual evoked potential (fVEP) shows a typical pattern development infancy(Kraemer & Sjöström ISCEV 1996) reflecting maturation of the afferent pathways. unspecific pathways develops first as ’late potential’, eventually forming P1 potential. later retino-geniculo-cortical afferents development, i.e....