A5048217357- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Diabetes and associated disorders
- Cancer Immunotherapy and Biomarkers
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Diabetes Management and Research
- Reproductive Biology and Fertility
- Spondyloarthritis Studies and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Viral-associated cancers and disorders
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Anodic Oxide Films and Nanostructures
- Pharmaceutical Practices and Patient Outcomes
- Cell Adhesion Molecules Research
- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Cytomegalovirus and herpesvirus research
Universitat Autònoma de Barcelona
2015-2025
Institute for Research in Biomedicine
2022
Hospital Del Mar
2019
Fundació Clínic per a la Recerca Biomèdica
2019
Center of Regenerative Medicine in Barcelona
2016
Centro de Biología Molecular Severo Ochoa
1999-2003
Universidad Autónoma de Madrid
1999-2003
Consejo Superior de Investigaciones Científicas
1999-2003
Hospital Universitari Germans Trias i Pujol
1992-1997
National Institute of Allergy and Infectious Diseases
1990-1994
Insulin-dependent diabetes mellitus (IDDM), in which only the pancreatic beta cells are destroyed by autoimmune response, is paradigm of organ-specific autoimmunity. As a result combination factors, number immunohistologic/cellular/molecular studies pancreas IDDM very limited. We report here conducted pancreata two patients: one newly diagnosed (case 1) and long standing 2). In case 1, we demonstrated presence morphologically normal viable without evidence viral infection. both cases...
The recognition of virus-infected cells by class I MHC-restricted cytotoxic T requires endogenous processing antigen for presentation. It is still unclear whether can be utilized II MHC molecules To test this possibility, a human B cell line expressing HLA-A2 and HLA-DR1 was infected with recombinant vaccinia virus the Influenza A M1 matrix protein (VAC-M1) assayed lysis different M1-specific cytolytic lines, restricted either or HLA-DR1. Class II-restricted VAC-M1-infected did occur. This...
Crohn's disease (CD) has been associated with an altered immune response to commensal microbiota, mostly based on increased seroreactivity microbial proteins. Although T cells are believed contribute the development of CD, little is known about antigens involved. We investigated antigen-specificity isolated from patients CD.We peripheral blood mononuclear 65 CD and 45 healthy individuals (controls). T-cell reactivity using proliferation assays (based thymidine incorporation...
This work addresses a biosensor combining the immunomagnetic separation and electrochemical biosensing based on intrinsic ALP activity of exosomes. approach explores for first time two different types biomarkers exosomes, in unique device biorecognition reaction: immunological enzymatic. Besides, alkaline phosphatase (ALP) exosomes as potential biomarker carcinogenesis well osseous metastatic invasion is also explored. To achieve that, an vitro model, from human fetal osteoblasts are used....
In the clinical course of Alzheimer's disease (AD) development, dementia phase is commonly preceded by a prodromal AD phase, which mainly characterized reaching highest levels Aβ and p-tau-mediated neuronal injury mild cognitive impairment (MCI) status. Because that, most cases are diagnosed when damage already established irreversible. Therefore, differential diagnosis MCI causes in these stages one greatest challenges for clinicians. Blood biomarkers emerging as desirable tools...
Exosomes are nanovesicles present in all the biological fluids, making them attractive as non-invasive biomarkers for diseases like cancer, among many others. However, exosomes complex to separate and detect, requiring comprehensive molecular characterization their routine use diagnostics. This study explores of peptides cost-effective stable alternatives antibodies exosome binding. To achieve that, phage display technology was employed select with high specificity target molecules exosomes....
Abstract We studied the morphology of cortical microvessels in brains 10 patients who had died after receiving a traumatic head injury (THI). Scanning electron microscopy (SEM) vascular corrosion casts, confocal histological sections immunocytochemistry, and detection apoptosis by terminal dUTP nick end labeling (TUNEL) were used. Microvascular casts showed an angioarchitectonic distribution that was defined as normal according to results obtained previous, nontraumatic series subjects....
Exosomes are receiving highlighted attention as new biomarkers for the detection of cancer since they profusely released by tumor cells in different biological fluids. In this paper, exosomes preconcentrated from serum immunomagnetic separation (IMS) based on a CD326 receptor specific epithelial cancer-related biomarker and detected glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts. Following lysis captured exosomes, GAPDH transcripts amplified reverse transcription polymerase...
Continuous treatment with ibrutinib not only exerts tumor control but also enhances T cell function in patients chronic lymphocytic leukemia (CLL). We conducted longitudinal multi-omics analyses samples from CLL receiving upfront to identify potential adaptive mechanisms Bruton tyrosine kinase (BTK) inhibition during the first 12 months of continuous therapy. found that induced a decrease expression exhaustion markers and proportion Tregs Tfh cells normalized levels observed healthy donors....
A lateral flow assay (LFA) is a paper-based, point-of-need test designed to detect specific analyte in complex samples low-resource settings. Although LFA has been successfully used different applications, its use still limited when high sensitivity required, especially the diagnosis of an early-stage condition. The limit detection (LOD) clearly related signal-generating system achieve visual readout, many cases involving nanoparticles coupled biomolecule, which, combined, provides and...
Abstract Endocrine epithelial cells, targets of the autoimmune response in thyroid and other organ-specific diseases, express HLA class II (HLA-II) molecules that are presumably involved maintenance regulation situ response. HLA-II thus expressed by cells have “compact” conformation therefore expected to stably bind autologous peptides. Using a new approach study T cell responses without characterization self-reactive their specificity, we identified natural HLA-DR-associated peptides organs...
Abstract Autoreactive T cells, responsible for the destruction of pancreatic β cells in type 1 diabetes, are known to have a skewed TCR repertoire NOD mouse. To define autoreactive cell human we searched intraislet monoclonal expansions from recent onset pancreas then trace them down patient’s peripheral blood and spleen. Islet infiltration was diverse, but five β-chain variable were detected Vβ1, Vβ7, Vβ11, Vβ17, Vβ22 families. identify any sequence bias TCRs intrapancreatic analyzed 139...
Exosomes are cell-derived nanovesicles released into biological fluids, which involved in cell-to-cell communication. The analysis of the content and surface exosomes allow conclusions about cells they originating from underlying condition, pathology or disease. Therefore, currently considered good candidates as biomarkers to improve current methods for clinical diagnosis, including cancer. However, due their low concentration, conventional procedures exosome detection biosensing usually...
The in vitro expansion and activation of T cells utilizing synthetic nanosized artificial antigen presenting (aAPCs) have emerged as a promising technique for cancer treatment. Although diverse nanomaterials been explored aAPC scaffolds, protein-only nanoparticles largely overlooked, despite their high designability biocompatibility. In this study, we exploit plug-and-play approach the development aAPCs using self-assembling properties ZapB coiled coil Z-domain antibody-capturing ability....
Reported alterations in T(reg) cells from type 1 diabetes (T1D) patients led us to a revision of their phenotypical features compared with controls. A fine cytometric analysis was designed for characterization, using panel markers including FOXP3, CTLA4, glucocorticoid-induced TNFR family related (GITR) and CD127. The frequency peripheral CD4(+)CD25(hi) similar between samples. However, the yield sorted significantly lower than When comparing T(reg)-cell phenotype samples, only difference...