A5005637301- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Diabetes and associated disorders
- SARS-CoV-2 and COVID-19 Research
- Phosphodiesterase function and regulation
- Renal Transplantation Outcomes and Treatments
- Toxin Mechanisms and Immunotoxins
- Animal Virus Infections Studies
- Mast cells and histamine
- CAR-T cell therapy research
- Adenosine and Purinergic Signaling
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2021-2023
Universitat de Barcelona
2021-2023
Universitat Autònoma de Barcelona
2013-2021
Inserm
2019
Nantes Université
2019
Center for Research in Transplantation and Translational Immunology
2019
Reported alterations in T(reg) cells from type 1 diabetes (T1D) patients led us to a revision of their phenotypical features compared with controls. A fine cytometric analysis was designed for characterization, using panel markers including FOXP3, CTLA4, glucocorticoid-induced TNFR family related (GITR) and CD127. The frequency peripheral CD4(+)CD25(hi) similar between samples. However, the yield sorted significantly lower than When comparing T(reg)-cell phenotype samples, only difference...
Development of a vaccine against HIV remains major target goal in the field. The recent success mRNA vaccines coronavirus SARS-CoV-2 is pointing out new era designs pathogens. Here, we have generated two types candidates HIV-1; one based on unmodified vectors and other 1-methyl-3′-pseudouridylyl modified expressing T cell multiepitopic construct including protective conserved epitopes from HIV-1 Gag, Pol Nef proteins (referred to as RNA-TMEP RNA-TMEPmod, respectively) defined their...
The development of new strategies to achieve a functional cure for HIV remains priority. We tested novel therapeutic vaccine using unmodified mRNA (TMEP-B) and modified by 1-methyl-3′-pseudouridylyl (TMEP-Bmod) expressing both multiepitopic sequences from Gag, Pol, Nef proteins, including different CD4 CD8 T-cell epitopes functionally associated with control in transfected monocyte-derived dendritic cells (MDDCs) obtained infected patients. In vitro assays were used test the mRNAs alone...
To reduce the use of non-specific immunosuppressive drugs detrimental to transplant patient health, therapies in development aim achieve antigen-specific tolerance by promoting regulatory T cells (Tregs). However, identification natural antigens recognized Tregs and contribution their dominance transplantation has been challenging. We identify epitopes derived from distinct major histocompatibility complex (MHC) class II molecules, sharing a 7-amino acid consensus sequence positioned central...
Many studies have shown that human natural killer T (NKT) cells can promote immunity to pathogens, but their regulatory function is still being investigated. Invariant NKT (iNKT) been be effective in preventing type 1 diabetes the NOD mouse model. Activation of plasmacytoid dendritic cells, modulation B-cell responses, and immune deviation were proposed responsible for suppressive effect iNKT cells. We studied capacity from control subjects patients with (T1D) at disease clinical onset....
The study of the effect cryopreservation on functionality monocyte-derived dendritic cells (MDDCs) and (DCs) is essential for their use in different clinical applications such as DCs-based vaccines. Its full maturation its optimal are crucial DCs based immunotherapy. In this study, we compared MDDCs derived from fresh cryopreserved PBMCs aspects phenotype T at level proliferation cytokine secretion. We pulsed obtained with two stimuli, CEF SEA, expression markers secretion were analyzed. Our...
Natural Killer T (NKT) cells play an important role in the immune response and can be activated by glycolipids presented CD1d protein. We present MCS-0208, unprecedented arylthioether-phytoceramide able to induce potent invariant NKT (iNKT) cell activation, notably when tested human iNKT cells. This arylsphingolipid analog has a simple phenyl group containing single hydroxyl substituent as surrogate of sugar ring. The phenylthioether structure contrasts with α-galactosylceramide (1),...
The COVID-19 pandemic has brought significant changes and advances in the field of vaccination, including implementation widespread use encapsidated mRNA vaccines general healthcare practice. Here, we present two new mRNAs expressing antigenic parts SARS-CoV-2 spike protein provide data supporting their functionality. first mRNA, called RBD-mRNA, encodes a trimeric form virus receptor binding domain (RBD). other termed T-mRNA, codes for relevant HLA I II epitopes. (COVARNA mRNAs) were...