A5080109257- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- SARS-CoV-2 and COVID-19 Research
- Vibrio bacteria research studies
- COVID-19 Clinical Research Studies
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Animal Virus Infections Studies
- Immune cells in cancer
- Microbial infections and disease research
- Respiratory viral infections research
- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Salmonella and Campylobacter epidemiology
- Immune Response and Inflammation
- Cancer Mechanisms and Therapy
- Bacterial Infections and Vaccines
- S100 Proteins and Annexins
- Diagnosis and treatment of tuberculosis
Texas Biomedical Research Institute
2020-2024
Biomedical Research Institute
2024
The University of Texas at San Antonio
2018-2022
Texas Center for Infectious Disease
2018-2022
University of Missouri Hospital
2015
Universitat de Barcelona
2011-2014
Vaccine and antiviral development against SARS-CoV-2 infection or COVID-19 disease would benefit from validated small animal models. Here, we show that transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) by the cytokeratin 18 promoter (K18 hACE2) represent a susceptible rodent model. K18 hACE2 succumbed to day 6, with virus detected in lung airway epithelium brain. ACE2 produced modest TH1/2/17 cytokine storm spleen peaked 2, an extended chemokine was both lungs This...
Despite great efforts put forward worldwide to combat the current coronavirus disease 2019 (COVID-19) pandemic, severe acute respiratory syndrome 2 (SARS-CoV-2) continues be a human health and socioeconomic threat. Insights into pathogenesis of SARS-CoV-2 contribution viral proteins outcome remain elusive.
Abstract There is a critical need for physiologically relevant, robust, and ready-to-use in vitro cellular assay platforms to rapidly model the infectivity of emerging viruses develop new antiviral treatments. Here we describe complexity human alveolar tracheobronchial air liquid interface (ALI) tissue models during SARS-CoV-2 influenza A virus (IAV) infections. Our results showed that both IAV effectively infect these ALI tissues, with exhibiting slower replication peaking at later...
ABSTRACT Vaccine and antiviral development against SARS-CoV-2 infection or COVID-19 disease currently lacks a validated small animal model. Here, we show that transgenic mice expressing human angiotensin converting enzyme 2 (hACE2) by the cytokeratin 18 promoter (K18 hACE2) represent susceptible rodent K18 hACE2-transgenic succumbed to day 6, with virus detected in lung airway epithelium brain. ACE2-transgenic produced modest TH1/2/17 cytokine storm spleen peaked 2, an extended chemokine was...
ABSTRACT In Shiga toxin-producing Escherichia coli (STEC), induction of toxin-encoding bacteriophages (Stx phages) causes the release free phages that can later be found in environment. The ability Stx to survive different inactivation conditions determines their prevalence environment, risk stx transduction, and generation new STEC strains. We evaluated infectivity genomes two (Φ534 Φ557) under conditions. Infectious were stable at 4, 22, 37°C pH 7 9 after 1 month storage but completely...
ABSTRACT Cytolethal distending toxin (Cdt) is produced by a variety of pathogenic bacteria, including serotypes Shiga toxin-producing Escherichia coli (STEC). The Cdt family comprises five variants (Cdt-I to Cdt-V) encoded three genes located within the chromosome or plasmids or, in case Cdt-I, bacteriophages. In this study, we evaluated occurrence cdt gene collection 140 environmental STEC isolates. was detected 12.1% strains, which strains carried inducible bacteriophages containing Cdt-V...
To discern if there was a particular genotype associated with clinical enteroaggregative Escherichia coli (EAEC) strains isolated from deployed military personnel (DMP) travelers' diarrhea, we characterized collection of EAEC DMP to three different geographical locations. Although did not identify specific TD in DMP, found that at the first clinic visit were more likely encode dispersin gene aap than collected follow-up visits. A majority isolates typical adhered HEp-2 cells, formed...
Vibrio cholerae is a Gram-negative waterborne human pathogen and the causative agent of cholera. Here, we present complete genome sequence seventh pandemic O1 biovar El Tor Inaba strain A1552 isolated in 1992. This clinical has served as an important model for studying cholera pathogenicity traits.
Live-attenuated vaccines (LAVs) are able to induce robust immune responses, including both humoral and cellular immunity, representing a very promising option provide broad long-term immunity. To develop LAVs for SARS-CoV-2, we engineered attenuated recombinant SARS-CoV-2 (rSARS-CoV-2) that simultaneously lacks the viral open reading frame 3a (ORF3a) in combination with either ORF6, ORF7a, or ORF7b (Δ3a/Δ6, Δ3a/Δ7a, Δ3a/Δ7b, respectively) proteins.
Tuberculosis (TB) infection, caused by the airborne pathogen Mycobacterium tuberculosis (M.tb), resulted in almost 1.4 million deaths 2019, and number of is predicted to increase 20% over next 5 years due COVID-19 pandemic. Upon reaching alveolar space, M.tb comes into close contact with lung mucosa before after its encounter host compartment cells. Our previous studies show that homeostatic, innate soluble components lining fluid (ALF) can quickly alter cell envelope surface upon contact,...
Abstract Escherichia coli ST1485 strains belong to the clinically important phylogroup F and have disseminated worldwide in humans, animals, environment. Here, we elucidated pathogenome of a global collection E. isolates from diverse sources retrieved public databases high-quality sequenced complete genome colistin-resistant strain CFSAN061771 isolated raw milk cheese which designated as reference strain. belongs O83:H42-ST1485 pathotype carries conjugative ColV plasmid, pCFSAN061771_01,...
ABSTRACT Two cytolethal distending toxin (Cdt) type V-encoding bacteriophages (Φ62 and Φ125) were induced spontaneously from their wild-type Escherichia coli strains the lysogens generated in Shigella sonnei . The stability of Cdt phages was determined at various temperatures pH values after 1 month storage by means infectivity tests using a plaque blot assay analysis phage genomes real-time quantitative PCR (qPCR): both highly stable. We assessed inactivation thermal treatment,...
Infections with globally disseminated Shiga toxin-producing Escherichia coli (STEC) of the O113:H21 serotype can progress to severe clinical complications, such as hemolytic uremic syndrome (HUS). Two phylogeographically distinct clonal complexes have been established by multi locus sequence typing (MLST). ST-820 isolates circulating exclusively in Australia caused human disease, HUS. Conversely, ST-223 prevalent US and outside seem rarely cause disease but are frequent contaminants....
Abstract The older adult population, estimated to double by 2050, is at increased risk of respiratory infections and other pulmonary diseases. Biochemical changes in the lung alveolar lining fluid (ALF) compartment cells can alter local immune responses as we age, generating opportunities for invading pathogens establish successful infections. Indeed, space adults a pro-inflammatory, pro-oxidative, dysregulated environment that remains understudied. We performed an exploratory, quantitative...
Some cdt genes are located within the genome of inducible or cryptic bacteriophages, but there is little information about mechanisms transfer because reduced number Cdt phages described. In this study, a new self-inducible Myoviridae phage (ΦAA91) was isolated from nonclinical O157:H7 Shiga toxin-producing Escherichia coli strain and used to lysogenize cdt-negative Shigella sonnei. We found that induced S. sonnei (ΦAA91-ss) not identical original phage. ΦAA91-ss infect collection 57...
The toxigenic conversion of Escherichia coli strains by Shiga toxin-converting (Stx) bacteriophages were prominent and recurring events in the stepwise evolution enterohemorrhagic E. (EHEC) O157:H7 from an enteropathogenic (EPEC) O55:H7 ancestor. Atypical, attenuated isolates have been described for both non-sorbitol fermenting (NSF) SF O157:NM serotypes, which are distinguished absence Stx, characteristic virulence hallmark Stx-producing (STEC). Such atypical either never acquired...
Tuberculosis is one of the leading causes death due to a single infectious agent. Upon infection,
Shigella sonnei is responsible for the majority of shigellosis infections in US with over 500,000 cases reported annually. Here, we present complete genome clinical multidrug resistant (MDR) strain 866, which highly susceptible to bacteriophage infections. The has a circular chromosome 4.85 Mb and carries 113 kb MDR plasmid. This IncB/O/K/Z-type plasmid, termed p866, confers resistance five different classes antibiotics including ß-lactamase, sulfonamide, tetracycline, aminoglycoside,...