A5000556958- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Microbial infections and disease research
- T-cell and B-cell Immunology
- Biochemical Acid Research Studies
- Metabolism and Genetic Disorders
- Viral Infections and Vectors
- T-cell and Retrovirus Studies
- Viral-associated cancers and disorders
- Genetic and phenotypic traits in livestock
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- Vector-borne infectious diseases
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Milk Quality and Mastitis in Dairy Cows
- Molecular Biology Techniques and Applications
- Biochemical and Molecular Research
- Banana Cultivation and Research
- Reproductive Physiology in Livestock
- Glycosylation and Glycoproteins Research
Moredun Research Institute
2015-2024
Vitenparken
2007-2019
Singapore University of Technology and Design
2015
Genomics (United Kingdom)
2000-2012
Royal Veterinary College
2012
The Pirbright Institute
2012
Cancer Research UK
2009
Roslin Institute
1995-2006
University of Sheffield
1987-1996
Albert B. Chandler Hospital
1996
Campylobacter jejuni produces a toxin called cytolethal distending (CDT). The genes encoding this in C. 81-176 were cloned and sequenced. nucleotide sequence of the revealed that there are three genes, cdtA, cdtB, cdtC, proteins with predicted sizes 30,11-6, 28,989, 21,157 Da, respectively. All found to be related Escherichia coli CDT proteins, yet amino acid sequences have diverged significantly. required for toxic activity HeLa cell assay. assays variety strains suggested most produce...
Summary An important prerequisite for a conservation programme is comprehensive description of genetic diversity. The aim this study was to use anonymous markers assess the between‐ and within‐population components diversity European pig breeds at scale whole continent using microsatellites. Fifty‐eight lines were analysed including local breeds, national varieties international commercial lines. A sample Chinese Meishan breed also included. Eleven additional from previous project added some...
Detailed biological analyses (e.g. epidemiological, genetic) of animal health and fitness in the field are limited by lack large-scale recording individual animals. An alternative approach is to identify immune traits that associated with these important functions can be subsequently used more detailed studies. We have an experimental dairy herd uniquely dense phenotypic data a range potentially useful correlated enhanced (or depressed) fitness. Blood samples from 248 cows were collected at...
The BoLA (bovine lymphocyte antigen) Nomenclature Committee met during the 1994 and 1996 conferences of International Society for Animal Genetics to define a sequence‐based nomenclature system genes system. rules acceptance new sequences are described names assigned sequenced alleles class II DRA, DRB1, DRB2, DRB3, DQA, DQB, DYA, DIB, DMA DMB . assignment I loci will be considered at later workshop when further sequencing/mapping data available.
Abstract Livestock abortion is an important cause of productivity losses worldwide and many infectious causes are zoonotic pathogens that impact on human health. Little known about the relative importance livestock in Africa, including subsistence farming communities critically dependent for food, income, wellbeing. We conducted a prospective cohort study abortion, supported by cross-sectional serosurveillance, to determine aetiologies abortions Tanzania. This approach generated several...
Ovine herpesvirus 2 (OvHV-2) is endemic in sheep populations worldwide and causes malignant catarrhal fever (MCF), a lymphoproliferative disease, cattle, bison deer. OvHV-2 has been placed the gammaherpesvirus subfamily related closely to Alcelaphine 1 (AlHV-1). Here, cloning, sequencing analysis of complete genome derived from lymphoblastoid cell line an affected cow (BJ1035) are reported. The unique portion consists 130,930 bp, with mean G+C content 52 mol%. DNA flanked by multiple copies...
In the course of investigating malignant catarrhal fever (MCF) subgroup rhadinoviruses, seven novel rhadinoviruses were identified in a variety ruminants, including domestic sheep, bighorn bison, black-tailed deer, mule fallow elk and addax. Based on DNA polymerase gene sequences, these newly recognized viruses clustered into second distinct ruminants with three members previously cattle, goats oryx. Phylogenetic analysis revealed that currently known ruminant appear to comprise genetic...
SUMMARY A major feature of the pathology induced by Theileria annulata is acute lymphocytic proliferation, and this study investigates mechanisms underlying intrinsic ability T. annulata-infected monocytes to induce naive autologous T cells proliferate. Different clones expressed different but constant levels MHC class II, varying from < 1.0 × 105 1.5 106 molecules/cell, as measured saturation binding. However, no correlation was found between level II expression cell proliferation....
Abstract The MHC of cattle encodes two distinct isotypes class II molecules, DR and DQ. Unlike humans, lack the DP locus about half common haplotypes express duplicated DQ genes. number frequency DQA DQB alleles means that most are heterozygous. If inter- and/or intrahaplotype pairing molecules occurs, carrying DQ-duplicated may more restriction elements than would be predicted by expressed alleles. We investigating whether cause differences in immune response, particularly terms generating...
While Bovine herpesvirus 1 (BoHV-1) has been known as a cause of bovine abortion for nearly 50 years, information is limited on the current prevalence BoHV-1 in United Kingdom, or about mode virus dissemination to infection fetus. The present study aimed investigate these issues by surveying cases and comparing diagnostic methods determine which are most efficient BoHV-1-induced abortion. Where DNA was detected, viral load compared fetal tissues, using real-time polymerase chain reaction...
Abstract Background Previous studies have proposed that mammalian toll like receptors (TLRs) evolved under diversifying selection due to their role in pathogen detection. To determine if this is the case, we examined extent of adaptive evolution TLR5 gene both individual species and defined clades mammalia. Results In support previous studies, find evidence TLR5. However, also show genes domestic livestock a concentration single nucleotide polymorphisms suggesting specific signature...
Infectious bovine rhinotracheitis (IBR) is the major disease caused by herpesvirus 1 (BoHV‐1), an important pathogen of cattle. Being alphaherpesvirus, virus establishes a lifelong latent infection in sensory nerve ganglia following primary infection. Latent can be reactivated various stressful stimuli, and subsequent re‐excretion infectious maintains within herd. BoHV‐1 to all cattle farmers because range diseases it cause barrier international trade; therefore, every herd health plan...
Protection of cattle from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever (MCF) has been described previously, using an attenuated virus vaccine in unlicensed adjuvant. The was hypothesised to induce a protective barrier virus-neutralising antibody the oro-nasal region, supported by observation high titre neutralising antibodies nasal secretions protected animals. Here we describe further analysis this strategy, studying effectiveness formulated with licensed adjuvant;...
Abstract: A rapid, high‐resolution sequence‐based typing (SBT) system for BoLA‐DRB3 exon 2 was developed. Amplification of the entire achieved by a fully nested PCR with locus‐specific primers and sequencing performed directly on product. Heterozygous sequence data were obtained automated analysis both alleles. Forward reverse assembled to improve identification all heterozygous positions. Specific software (Haplofinder, Roslin Institute Software, Roslin, UK) designed allele assignment....
The gammaherpesvirus alcelaphine herpesvirus 1 (AlHV-1) causes malignant catarrhal fever in susceptible ungulates but infects its natural host, wildebeest, without obvious clinical signs. In tissue culture, AlHV-1 is initially predominantly cell associated and virulent on extended culture becomes cell-free attenuated. We wanted to determine what changes protein composition had taken place during the transition from attenuated virus culture. Purified preparations were fractionated by sodium...