A5050230143- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Pancreatic function and diabetes
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Diabetes and associated disorders
- Antibiotic Resistance in Bacteria
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Advanced Nanomaterials in Catalysis
- Melanoma and MAPK Pathways
- Coenzyme Q10 studies and effects
- Cancer, Hypoxia, and Metabolism
- Diabetes Management and Research
- Psoriasis: Treatment and Pathogenesis
- Cardiac Imaging and Diagnostics
- RNA regulation and disease
- Adenosine and Purinergic Signaling
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
National Cerebral and Cardiovascular Center
2020-2025
Kyoto University
2024
Osaka University
1990-2023
Japan Science and Technology Agency
2019
SPring-8
2008
Bradyarrhythmia is a common clinical manifestation. Although the majority of cases are acquired, genetic analysis families with bradyarrhythmia has identified growing number causative gene mutations. Because only ultimate treatment for symptomatic been invasive surgical implantation pacemaker, discovery novel therapeutic molecular targets necessary to improve prognosis and quality life.We investigated family containing 7 individuals autosomal dominant bradyarrhythmias sinus node dysfunction,...
Oxidative phosphorylation defects result in now intractable mitochondrial diseases (MD) with cardiac involvement markedly affecting prognosis. The mechanisms underlying the transition from compensation to dysfunction response metabolic deficiency remain unclear. Here, we used spatially resolved transcriptomics and single-nucleus RNA sequencing (snRNA-seq) on heart of a patient cardiomyopathy (MCM), combined an MCM mouse model cardiac-specific Ndufs6 knockdown (FS6KD). Cardiomyocytes...
DNA recombination events need to be strictly regulated, because an increase in the recombinational frequency causes unfavorable alteration of genetic information. Recent studies revealed existence a novel anti-recombination enzyme, MutS2. However, mechanism by which MutS2 inhibits homologous has been unknown. Previously, we found that Thermus thermophilus (ttMutS2) harbors endonuclease activity and this is confined C-terminal domain, whose amino acid sequence widely conserved variety...
Article29 November 2020Open Access Transparent process AMPK regulates cell shape of cardiomyocytes by modulating turnover microtubules through CLIP-170 Shohei Yashirogi Department Medical Biochemistry, Osaka University Graduate School Frontier Biological Science, Suita, Osaka, JapanThese authors contributed equally to this work Search for more papers author Takemasa Nagao Japan Molecular Pharmacology, National Cerebral and Cardiovascular Center, Yuya Nishida Yusuke Takahashi Tasneem Qaqorh...
In eukaryotes, cleavage and polyadenylation specificity factor (CPSF) is an important multisubunit complex required for 3′-end of the transcript as well subsequent during maturation mRNA.1 this family protein factors, CPSF-73 CPSF-100 are members metallo-β-lactamase superfamily a ribonuclease involved in mRNA.1, 2 β-CASP proteins widely distributed bacteria, archaea belong to group novel nuclease superfamily.3 named after metallo-β-lactamase-associated CPSF Artemis SNM1/PSO2, they commonly...
Abstract The respiratory chain (RC) transports electrons to form a proton motive force that is required for ATP synthesis in the mitochondria. RC disorders cause mitochondrial diseases have few effective treatments; therefore, novel therapeutic strategies are critically needed. We previously identified Higd1a as positive regulator of cytochrome c oxidase (CcO) RC. Here, we test has beneficial effect by increasing CcO activity models dysfunction. first demonstrated tissue‐protective effects...
// Soyoko Morimoto 1 , Fumihiro Fujiki 2 Kenta Kondo 3 Hiroko Nakajima Yoshiki Kobayashi Miki Inatome Nao Aoyama Yuya Nishida Akihiro Tsuboi Yoshihiro Oka 4, 5, 6 Sumiyuki 5 Jun Nakata Naoki Hosen 4 Yusuke Oji and Haruo Sugiyama Department of Cancer Immunotherapy, Osaka University Graduate School Medicine, Osaka, Japan Immunology, Functional Diagnostic Science, Stem Cell Biology, Respiratory Medicine Clinical Immunopathology, Immunology Frontier Research Center (World Premier International...
The effects of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the growth and DNA synthesis cultured human keratinocytes obtained from involved uninvolved psoriatic epidermis normal were studied. Treatment with 10(-8) M 10(-7) 1,25(OH)2D3 inhibited cell as follows: 58.5 +/- 19.3% 21.3 13.6% in (n = 6); 43.8 22.8% 17.8 12.3% 4); 51.7 18.2% 13.2 6.4% 6). Inhibition was virtually complete at 10(-6) M. also by M, 70.0 8.3%, 59.0 6.8% 16.7 4.0%, respectively, 3); 78.5 13.5%, 51.5 25.5% 24.5 21.5%, 2);...
Effects of interfacial interactions on the electrocatalytic activity protein-tethered bilayer lipid membranes (ptBLMs) containing cytochrome c oxidase (CcO) for oxygen reduction reaction are studied by using protein film electrochemistry and surface-enhanced infrared absorption (SEIRA) spectroscopy. Mammalian CcO was immobilized a gold electrode via self-assembled monolayers (SAMs) mixed alkanethiols. The orientation is controlled SAM-CcO critical to (cyt c) binding. CcO-phospholipid CcO-cyt...
Asymmetric diadenosine tetraphosphate (Ap(4)A) hydrolases degrade the metabolite Ap(4)A back into ATP and AMP. The three-dimensional crystal structure of hydrolase (16 kDa) from Aquifex aeolicus has been determined in free ATP-bound forms at 1.8 1.95 A resolution, respectively. overall enzyme shows an alphabetaalpha-sandwich architecture with a characteristic loop adjacent to catalytic site protein molecule. molecule is bound primary active adenine moiety nucleotide binds ring-stacking...
Abstract Antimicrobial resistance (AMR) is a global health problem. Despite the enormous efforts made in last decade, threats from some species, including drug-resistant Neisseria gonorrhoeae , continue to rise and would become untreatable. The development of antibiotics with different mechanism action seriously required. Here, we identified an allosteric inhibitory site buried inside eukaryotic mitochondrial heme-copper oxidases (HCOs), essential respiratory enzymes for life. steric...
<h3>Background:</h3> Bacterial translocation across the gut barrier has been implicated in autoimmune diseases, including systemic lupus erythematosus (SLE). However, it remains unclear how translocated bacteria contribute to development of autoimmunity. <h3>Objectives:</h3> The present study aimed elucidate role commensal context molecular mimicry, by utilizing model mice and blood samples from untreated patients with SLE. <h3>Methods:</h3> We have previously generated C57BL/6 Zap70skg/skg...
Right ventricular dysfunction is a key clinical issue for the viability of donation-after-circulatory-death (DCD) heart transplantation. DCD hearts with volume overload have potential to exhibit aggravated right following The c-jun/c-fos mRNAs are genes that immediately respond myocardial cell stretch. We assessed stretch during asphyxia-induced cardiac arrest by measuring mRNA expression levels. trachea was dissected and ligated initiate asphyxiation in anesthetized Wistar rats under...
CD4+ T cells that recognize antigenic peptides presented on HLA class II are essential for inducing an optimal anti-tumor immune response, and adoptive transfer of tumor antigen-specific TCR-transduced with high responsiveness against is a promising strategy cancer treatment. Whereas precise evaluation method functional avidity, indicator cell tumors, has been established I-restricted TCRs, it remains unestablished II-restricted TCRs. In this study, we generated novel platform line, CD4-2D3,...
In prokaryotic cells, genomic DNA forms an aggregated structure with various nucleoid-associated proteins (NAPs). The functions of are cooperatively modulated by NAPs, which HU is considered to be one the most important. binds double-stranded (dsDNA) and serves as a structural modulator in genome architecture. It plays important roles diverse functions, including replication, segregation, transcription repair. Interestingly, it has been reported that also single-stranded (ssDNA) regardless...
Summary Oxidative phosphorylation defects results in mitochondrial diseases, with cardiac involvement markedly impacting prognosis. However, the mechanisms underlying transition from compensation to dysfunction response metabolic deficiency remain unclear, impeding development of effective treatments. Here, we employed single-nucleus RNA sequencing (snRNA-seq) on hearts cardiomyopathy (MCM) mice cardiac-specific Ndufs6 knockdown (FS6KD). Pseudotime trajectory analysis cardiomyocytes early...
Aims: In vivo studies have demonstrated that pancreatic α-cells potency to transdifferentiate into insulin-producing β cells, which could lead potential therapy cure diabetes. addition, inappropriate glucagon secretion from α cells has been shown be involved in the pathophysiology of Although such findings highlight need for better understanding α-cell biology, it remains elucidated where originate and what regulates cell fate: neogenesis, maturation dedifferentiation. To address this issue,...
Summary AMP-activated protein kinase (AMPK) is a multifunctional that regulates microtubule (MT) dynamic instability through CLIP-170 phosphorylation; however, its physiological relevance in vivo remains to be elucidated. In this study, we identified an active form of AMPK localized at the intercalated discs heart, specific cell-cell junction present between cardiomyocytes. A contractile inhibitor, MYK-461, prevented localization discs, and effect was reversed by removal suggesting regulated...