A5101442315- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- Breast Cancer Treatment Studies
- AI in cancer detection
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Monoclonal and Polyclonal Antibodies Research
- Congenital heart defects research
- Metaheuristic Optimization Algorithms Research
- Digital Imaging for Blood Diseases
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- Advanced Steganography and Watermarking Techniques
- Nutrition, Genetics, and Disease
- Bioactive Compounds and Antitumor Agents
- Advanced Manufacturing and Logistics Optimization
- Evolutionary Algorithms and Applications
- Assembly Line Balancing Optimization
- RNA Interference and Gene Delivery
- Scheduling and Optimization Algorithms
- melanin and skin pigmentation
Chubu University
2014-2025
Osaka University
2010-2022
Okayama University
2011-2013
The University of Texas MD Anderson Cancer Center
2011-2013
Okayama Rosai Hospital
2013
Okayama University Hospital
2012-2013
Kagawa Prefectural Central Hospital
2013
Okayama Saiseikai General Hospital
2013
Mizushima Central Hospital
2013
St. Luke's International Hospital
2013
DiGeorge syndrome chromosomal region 8 ( Dgcr8 ), a candidate gene for 22q11.2 deletion-associated schizophrenia, encodes an essential component microRNA (miRNA) biosynthesis that plays pivotal role in hippocampal learning and memory. Adult neurogenesis is known to be important hippocampus-dependent memory, but the molecular mechanisms of adult schizophrenia remain unclear. Here, we show heterozygosity mice leads reduced cell proliferation hippocampus, as well impaired learning. Several...
Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t essential for very early stages of spermatogenesis. gene deficiency leads to azoospermia because the loss haploid germ cells. When differentiating spermatogonia emerge normal spermatogenesis, appears replaces canonical proteins. Structural biochemical analyses reveal...
22q11 deletion syndrome (22q11DS) frequently accompanies psychiatric conditions, some of which are classified as schizophrenia and bipolar disorder in the current diagnostic categorization. However, it remains elusive how chromosomal microdeletion leads to mental manifestation at mechanistic level. Here we show that a 22q11DS mouse model with 18 orthologous genes human (Df1/+ mice) has deficits migration cortical interneurons hippocampal dentate precursor cells. Furthermore, Df1/+ mice...
Abstract The potential role of Thy-1 in CD3/TCR complex-mediated signal delivery to murine thymocytes was studied. Ag-mimicking cross-linked anti-CD3 mAb stimulated suspension from adult (6 8 wk old) mice for a brisk free cytoplasmic calcium ion ([Ca2+]i) rise, low level inositol phosphate production, and marginal increase tyrosine-specific phosphorylation 110/120-kDa 40-kDa cellular proteins. Weak but sustained [Ca2+]i weak protein tyrosine were also induced by the anti-Thy-1 that mimicked...
Recently, miR-143 and miR-145 have been shown to belong a subset of microRNAs whose expression is controlled by complex tumor suppressor p53 DEAD-box RNA helicase subunits p68/p72. While accumulating studies acknowledged that both miRNAs function as suppressors are similarly regulated, evidence their coordinated action against tumorigenesis has poorly presented. Herein, we establish transgenic mice express under the control CAG regulatory unit. When crossbred with ApcMin/+ mice, development...
The Caenorhabditis elegans heterochronic gene pathway, which consists of a set regulatory genes, plays an important role in the timing stage-specific cell lineage development nematodes. Research into pathway gave rise to landmark microRNA (miRNA) studies and showed that these genes are stem cancer biology; however, their functions vertebrate largely unknown. To elucidate function during development, we cloned zebrafish homologs C. let-7 miRNA-binding protein, Lin-28, analyzed development....
Abstract Clustered regularly interspaced palindromic repeat (CRISPR)/CRISPR-associated protein (Cas)-based in vivo chromosomal rearrangements are a promising approach for generating model organisms with specific abnormalities. However, conventional methods rely on viral vectors, which expensive, require specialized equipment, and pose potential safety risks, thereby limiting their widespread application. To overcome the limitations above, we developed novel, efficient, cost-effective...
Abstract The PSD‐95/Dlg/ZO‐1 (PDZ) domain‐containing proteins MALS and PSD‐95 localize to post‐synaptic densities bind the COOH‐termini of NR2 subunits NMDA receptor. effects MALS‐2 on channel activity receptors were compared using Xenopus oocyte expression system. Both increased current response NR1‐NR2B receptor l ‐glutamate. In contrast, NR1‐NR2A was by but not MALS‐2. had no effect either potentiation or protein kinase C, Src‐mediated activity, whereas almost completely inhibited these...
Abstract The signal delivery pathway triggered by crosslinking CD3 and Thy-1 together (CD3/Thy-1 crosslinkage) on murine thymocytes for cellular DNA fragmentation/growth inhibition was analyzed. treatment of with herbimycin A as a specific tyrosine kinase inhibitor under suboptimum conditions before the CD3/Thy-1 crosslinkage partially but preferentially inhibited otherwise promoted phosphorylation p40 p56. Evidence then provided that acceleration activity p56lck involved in...
Abstract Protein kinase C (PKC) has been believed to play an important role in the differentiation/proliferation of various kinds mammalian cells. To analyze its function living animals, we have established a transgenic mouse line carrying rabbit protein alpha cDNA under control regulatory element human CD2. Thymocytes these mice overexpressed PKC alpha. Interestingly, increase was detected mainly membrane fractions thymocytes. Although thymocytes did not show any distinct proliferative...