A5028188190- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Genomics and Chromatin Dynamics
- 3D Printing in Biomedical Research
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive biology and impacts on aquatic species
- Genetic Syndromes and Imprinting
- Viral Infectious Diseases and Gene Expression in Insects
- Reproductive Health and Technologies
- Radiopharmaceutical Chemistry and Applications
- Trace Elements in Health
- Urological Disorders and Treatments
- Hypothalamic control of reproductive hormones
- Medical Imaging Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Antioxidant Activity and Oxidative Stress
Fuso Pharmaceutical Industries (Japan)
2015-2025
Kindai University
2015-2025
Yokohama City University
2023
Osaka University of Pharmaceutical Sciences
2019
Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t essential for very early stages of spermatogenesis. gene deficiency leads to azoospermia because the loss haploid germ cells. When differentiating spermatogonia emerge normal spermatogenesis, appears replaces canonical proteins. Structural biochemical analyses reveal...
In mammals, DNA is methylated at CpG sites, which play pivotal roles in gene silencing and chromatin organization. Furthermore, methylation undergoes dynamic changes during development, differentiation, pathological processes. The conventional methods represent snapshots; therefore, the dynamics of this marker within living organisms remains unclear. To track dynamics, we made a knockin mouse that expresses red fluorescent protein (RFP)-fused methyl-CpG-binding domain (MBD) from ROSA26 locus...
We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted α-minimum essential (α-MEM), supplemented with Knockout serum replacement (KSR) or AlbuMAX, neither which were defined chemically. In this study, we formulated a chemically (CDM) that can induce under organ conditions. It was found bovine albumin (BSA) purified through three different procedures had effects on spermatogenesis. also...
Abstract In the pre-implantation embryo, aneuploidy resulting from chromosome segregation error is considered responsible for pregnancy loss. However, only a few studies have examined relationship between errors during early cleavage and development. Here, we evaluated this by live-cell imaging using histone H2B-mCherry probe subsequent single blastocyst transfer mouse embryos obtained in vitro fertilization. We showed that some exhibiting chromosomal formation of micronuclei retained their...
Nuclear/chromosomal integrity is an important prerequisite for the assessment of embryo quality in artificial reproductive technology. However, lipid-rich dark cytoplasm bovine embryos prevents its observation by visible light microscopy. We performed live-cell imaging using confocal laser microscopy that allowed long-term nuclear/chromosomal dynamics vitro fertilised (IVF) embryos. analysed relationship between aberrations and embryonic development morphological blastocyst quality....
Zygotes require two accurate sets of parental chromosomes, one each from the mother and father, to undergo normal embryogenesis. However, upon egg–sperm fusion in vertebrates, zygote has three sperm egg. The therefore eliminates set maternal chromosomes (but not paternal chromosomes) into polar body through meiosis, but how are protected meiosis been unclear. Here we report that RanGTP F-actin dynamics prevent proximity chromosomes. prevents localization Juno CD9, egg membrane proteins...
Abstract An in vitro spermatogenesis method using mouse testicular tissue to produce fertile sperm was established more than a decade ago. Although this culture has generally not been effective other animal species, we recently succeeded improving the condition induce of rats up round spermatid stage. In present study, introduced acrosin-EGFP transgenic order clearly monitor production haploid cells during vitro. addition, metabolomic analysis media cultivation revealed metabolic dynamics...
In preimplantation embryos, an abnormal chromosome number causes developmental failure and a reduction in the pregnancy rate. Conventional testing methods requiring biopsy reduce risk of associated genetic diseases; nevertheless, cell also reduces Therefore, we attempted to count chromosomes mouse embryos using super-resolution live-cell imaging as new method counting that does not or viability. We counted 40 at first mitosis by injecting with histone H2B-mCherry mRNA under conditions which...
In assisted reproduction, many factors in the culture environment, including light, temperature, pH, and media, can reduce preimplantation embryo viability. Laboratory glassware is also a known risk factor for vitro embryos; however, underlying mechanisms that disrupt embryonic development remain unclear. We identified Zn eluted from as an embryotoxic substance. mouse embryos, induced delayed development, abnormalities chromosome segregation, cytokinesis, zygotic gene activation (e.g....
In vitro mouse spermatogenesis using a classical organ culture method became possible by supplementing basal medium with only the product of bovine serum albumin purified chromatography (AlbuMAX), which indicated that AlbuMAX contained every chemical factor necessary for spermatogenesis. However, since identity these factors was unclear, improvements in media and our understanding nutritional signal substances required were hindered. present study, chemically defined (CDM) without used to...
Journal Article MICROSCOPIC OBERVATIONS OF PARTHENOGENETIC EMBRYONIC TISSUES: From Virgin Turkey Eggs Get access T. S. YAO, YAO Search for other works by this author on: Oxford Academic PubMed Google Scholar MARLOW W. OLSEN *United States Department of Agriculture Heredity, Volume 46, Issue 3, May 1955, Pages 133–134, https://doi.org/10.1093/oxfordjournals.jhered.a106540 Published: 01 1955
Abstract To improve the performance of assisted reproductive technology, it is necessary to find an indicator that can identify and select embryos will be born or aborted. We searched for indicators embryo selection by comparing born/abort mouse embryos. found asynchronous during 4–8-cell stage were predisposed In embryos, nuclear translocation YAP1 in some blastomeres compaction delayed, number ICMs was reduced. Hence, possible have abnormal differentiation. When synchrony human observed,...
Systematic studies of mouse embryo culture beginning in 1949 led to an understanding essential medium components for early mammalian embryos, and from the zygote blastocyst stage was achieved 1968. Since then, that are either beneficial or detrimental have been identified. A variety media mimic female reproductive tract, such as human tubal fluid sequential media, were developed 1970s 1990s, a single which concentrations determined by simplex optimization method introduced clinical use 2002....
Our aim is to make an ideal embryo culture medium close human oviduct fluid (HOF) components, and evaluate the quality of this with clinical outcomes in assisted reproductive technology (ART) by a prospective randomized controlled trial (RCT).
Oocytes without a zona pellucida (ZP) often occur as result of congenital or operational effects, but they are difficult to handle, and embryonic survival is low. Such zona-free (ZF) oocytes therefore not used in clinics laboratories. Furthermore, the laboratory, removal ZP necessary for genetic manipulation by viral infection twin production blastomere separation, adverse effects on development have been reported. It would be extremely valuable if embryo could covered with structure similar...
Incorporation of bovine serum-derived albumin formulation (AlbuMAX) into a basic culture medium, MEMα, enables the completion in vitro spermatogenesis through testicular tissue mice. However, this medium was not effective other animals. Therefore, we sought an alternative approach for using synthetic without AlbuMAX and aimed to identify its essential components. In addition factors known be important spermatogenesis, such as retinoic acid reproductive hormones, found that antioxidants...
Abstract: Systematic studies of mouse embryo culture beginning in 1949 led to an understanding essential medium components for early mammalian embryos, and from the zygote blastocyst stage was achieved 1968. Since then, that are either beneficial or detrimental have been identified. A variety media mimic female reproductive tract, such as human tubal fluid sequential media, were developed 1970s 1990s, a single which concentrations determined by simplex optimization method introduced clinical...
Abstract A simple method for producing pseudopregnant mice supports pup production. In this study, pregnant ICR were obtained without mating with vasectomized via administration of mouse Kisspeptin‐10 (mKp‐10) and transferring blastocysts to the uterus. Blastocyst transfer after mKp‐10 gapping reddish pink vagina resulted in 65.2% (15/23) pregnancies, 39.1% (34/87) transferred showed full‐term growth. Vaginal smears observed accurate estrus cycle determination, subsequent mKp10 during stage...
Ovarian stimulation protocols are widely used to collect oocytes in assisted reproductive technologies (ARTs). Although the influence of ovarian on embryo quality has been described, this issue remains controversial. Here, we analyzed developmental speed and chromosome segregation using live cell imaging. Female mice at proestrus stage were separated by appearance vagina as non-stimulation (–) group, other administered pregnant mare serum gonadotropin (PMSG) human chorionic (hCG) (+) groups....
Selection of high-quality embryos is critical in assisted reproductive technology (ART), but it relies on visual assessment by experts, and the birth rate remains low. We previously developed a deep learning method to predict mouse quantifying morphological features cell nuclei. This involves nuclear segmentation fluorescence microscopy images, labeling nuclei not feasible medical applications. Here, we FL2-Net, for time-series three-dimensional bright-field images without labeling. FL2-Net...