A5102852926- Genetic diversity and population structure
- Fish Ecology and Management Studies
- Reproductive Biology and Fertility
- Environmental DNA in Biodiversity Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Identification and Quantification in Food
- Epigenetics and DNA Methylation
- Evolution and Genetic Dynamics
- Genomics and Phylogenetic Studies
- Prenatal Screening and Diagnostics
- Insect and Arachnid Ecology and Behavior
- Marine Bivalve and Aquaculture Studies
- Chromosomal and Genetic Variations
- Isotope Analysis in Ecology
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Aquaculture disease management and microbiota
- Genetic and phenotypic traits in livestock
- Pluripotent Stem Cells Research
- Wnt/β-catenin signaling in development and cancer
- melanin and skin pigmentation
- Birth, Development, and Health
- Marine and fisheries research
- Urinary Bladder and Prostate Research
- Microtubule and mitosis dynamics
Kyushu University
2024-2025
Kyoto University
1987-2024
RIKEN Center for Biosystems Dynamics Research
2020-2024
Southern Cross University
2019
Although domesticated goldfish strains exhibit highly diversified phenotypes in morphology, the genetic basis underlying these is poorly understood. Here, based on analysis of transposable elements allotetraploid genome, we found that its two subgenomes have evolved asymmetrically since a whole-genome duplication event ancestor and common carp. We conducted sequencing 27 wild goldfish. identified more than 60 million variations established population structure major strains. Genome-wide...
Abstract Acentrosomal meiosis in oocytes represents a gametogenic challenge, requiring spindle bipolarization without predefined bipolar cues. While much is known about the structures that promote acentrosomal microtubule nucleation, less mediate mammalian oocytes. Here, we show mouse oocytes, kinetochores are required for I. This process promoted by oocyte-specific, microtubule-independent enrichment of antiparallel crosslinker Prc1 at via Ndc80 complex. In contrast, II, cytoplasm contains...
Abstract Chromosome segregation errors in oocytes lead to the production of aneuploid eggs, which are leading cause pregnancy loss and several congenital diseases such as Down syndrome. The frequency chromosome increases with maternal age, especially at a late stage reproductive life. How aging various life stages affects differently remains poorly understood. In this study, we describe aging‐associated changes transcriptome profile mouse throughout Our single‐oocyte comprehensive RNA...
Zygotes require two accurate sets of parental chromosomes, one each from the mother and father, to undergo normal embryogenesis. However, upon egg–sperm fusion in vertebrates, zygote has three sperm egg. The therefore eliminates set maternal chromosomes (but not paternal chromosomes) into polar body through meiosis, but how are protected meiosis been unclear. Here we report that RanGTP F-actin dynamics prevent proximity chromosomes. prevents localization Juno CD9, egg membrane proteins...
Abstract Mammalian oocytes undergo a long‐term meiotic arrest that can last for almost the entire reproductive lifespan. This occurs after DNA replication and is prolonged with age, which poses challenge to in maintaining replication‐dependent chromosomal proteins required completion of meiosis. In this study, we show histones are reduced age mouse oocytes. Both types histone H3 variants, H3.1/H3.2 replication‐independent H3.3, decrease age. Aging‐associated reduction associated...
Primordial follicle activation (PFA) is a pivotal event in female reproductive biology, coordinating the transition from quiescent to growing follicles. This study employed comprehensive single-cell RNA sequencing gain insights into detailed regulatory mechanisms governing synchronized dormancy and between granulosa cells (GCs) oocytes with progression of PFA process. Wntless ( Wls ) conditional knockout (cKO) mice served as unique model, suppressing pre-GCs GCs, disrupting somatic...
Bipolar spindle assembly and chromosome biorientation are prerequisites for segregation during cell division. The kinesin motor KIF11 drives bipolarization by sliding antiparallel microtubules bidirectionally, elongating a spherical into bipolar-shaped structure in oocytes. This process stretches chromosomes, establishing at the equator. quantitative requirement remains unclear. Here, using genetic strategy to modulate expression levels, we show that Kif11 haploinsufficiency impairs...
Floodplains harbor highly biodiverse ecosystems, which have been strongly affected by both past climate change and recent human activities, resulting in a high prevalence of many endangered species these habitats. Understanding the history floodplain over wide range timescales can contribute to effective conservation planning. We reconstructed population formation Itasenpara bitterling Acheilognathus longipinnis, an fish Japan, broad timescale based on phylogenetic analysis, demographic...
Asexual vertebrates are rare and at risk of extinction due to their restricted adaptability through the loss genetic recombination. We explore mechanisms behind generation maintenance diversity in triploid asexual (gynogenetic) Carassius auratus fish, which is widespread East Asian fresh waters exhibits one most extensive distribution among despite its dependence on host sperm. Our analyses composition using dozens markers genome-wide transcriptome sequencing uncover admixed Japanese...
AEI Aquaculture Environment Interactions Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 11:657-669 (2019) - DOI: https://doi.org/10.3354/aei00338 Oyster larvae as a potential first feed for small-mouthed ornamental larval fish Alexander J. Basford*, Benjamin Mos, Tomoki Mishina, Symon A. Dworjanyn National Marine Science Centre, Southern Cross University, Coffs Harbour, NSW 2450, Australia...
近年の京都府鴨川では, 天然アユの溯上が観察されるようになったが, 多くの落差工や堰堤がアユの溯上を妨げていることから, 仮設魚道による改善が試みられている. 本研究では, 鴨川の中下流部におけるアユの流程分布の現状を明らかにするために, 水中目視観察による調査をおこなった. また, 夏期には下流部の水温環境がアユの成育に適さない高水温となることから, その影響を把握するために, アユの生息場所利用状況, 摂餌行動, ならびに栄養状態の調査を行った. その結果, 天然遡上と考えられるアユは鴨川の最下流から約 15 km 上流まで観察され, 水温がアユの生息適温を大幅に超える下流部でも多くのアユが生息していることがわかった. 鴨川下流部では, 朝方に平瀬の流心で摂餌をしていたアユが昼間には湧水のある砂州尻ワンドに集合している現象が観察された. 下流部では午後の高水温時に胃内容物量が少なかったことから, 摂餌時間も中流部に比べて短いと推察された. さらに, 下流部のアユについては中流部よりも小さい個体の割合が高く, 大型個体では,...
Abstract Countershading, characterized by a darker dorsal surface and lighter ventral surface, is common among many animals. This dorsoventral pigment polarity often thought to be adaptive coloration for camouflage. By contrast, noncountershaded (melanistic) morphs occur within species due genetic color polymorphism in terrestrial However, the with either countershaded or melanistic poorly known wild aquatic study explored nature of diverged lineage gudgeon fish (genus Sarcocheilichthys )...
Abstract Primordial follicle activation (PFA) is a pivotal event in female reproductive biology, coordinating the transition from quiescent to growing follicles. This study employed comprehensive single-cell RNA sequencing gain insights into detailed regulatory mechanisms governing synchronized dormancy and between granulosa cells (GCs) oocytes with progression of PFA process. Wntless ( Wls ) conditional knockout (cKO) mice served as unique model, suppressing pre-GCs GCs, disrupting somatic...
Abstract It is known that some endangered species have persisted for thousands of years despite their very small effective population sizes and low levels genetic polymorphisms. To understand the mechanisms long-term persistence in threatened species, we determined whole genome sequences akame (Lates japonicus), which has survived a long time with extremely variations. Genome-wide heterozygosity was estimated to be 3.3 3.4 × 10−4/bp, one smallest values teleost fishes. Analysis demographic...
Abstract Hybridization and introgression between closely related species significantly affect their evolutionary ecological processes. Understanding the ecological, environmental, geographical conditions that promote occurrence is imperative. It hypothesized inhabiting geologically unstable habitats or with life-history constraints prevent evasion from such environments are more prone to interspecific hybridization, due limited development of prezygotic isolation. To test this hypothesis, we...
Abstract Accurate knowledge of spatial population structure is crucial for species conservation and management, but low genetic diversity endangered makes it difficult to ascertain this information. In the Japanese lates or akame, Lates japonicus , an fish known have extremely among vertebrates, mtDNA AFLP marker analyses failed clearly partition individuals into two main distribution areas (Kochi Miyazaki prefectures), although a weak significant difference between these regions was...
Conservation of sperm-dependent asexual (gynogenetic) species is challenging due to their complicated ecological dynamics, which requires the stable coexistence with sperm-providing sexual relatives, who often share similar niches. A symbolic but vulnerable gynogenetic animal scarlet Carassius fish, or Hibuna, mainly found in Lake Harutori on Hokkaido, Japan. Although Hibuna has been protected as a symbol Natural Monument Japan, it recently suffered population decline. To establish an...