A5066544282- Meteorological Phenomena and Simulations
- Cardiovascular Function and Risk Factors
- Plant Water Relations and Carbon Dynamics
- Heart Failure Treatment and Management
- Cardiomyopathy and Myosin Studies
- Wind and Air Flow Studies
- Atmospheric and Environmental Gas Dynamics
- Aeolian processes and effects
- Endoplasmic Reticulum Stress and Disease
- Climate variability and models
- Oceanographic and Atmospheric Processes
- Remote Sensing and Land Use
- Cardiac electrophysiology and arrhythmias
- Cardiac Fibrosis and Remodeling
- ATP Synthase and ATPases Research
- Urban Heat Island Mitigation
- Ocean Waves and Remote Sensing
- Mitochondrial Function and Pathology
- Tropical and Extratropical Cyclones Research
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- Cardiac Ischemia and Reperfusion
- Signaling Pathways in Disease
- Metabolism, Diabetes, and Cancer
- Advanced Surface Polishing Techniques
Osaka University
2015-2025
Hyogo University
2023-2024
Ube Frontier University
2020-2024
Hyogo Medical University
2023-2024
Medical Research Foundation
2024
Kumamoto University
2019
Abbott (Sweden)
2015
Valve (United States)
2015
Okayama University
1993-2014
Yokohama Rosai Hospital
2013
The endoplasmic reticulum (ER) is recognized as an organelle that participates in folding secretory and membrane proteins. ER responds to stress by upregulating chaperones, but prolonged and/or excess leads apoptosis. However, the potential role of pathophysiological hearts remains unclear.Mice were subjected transverse aortic constriction (TAC) or sham operation. Echocardiographic analysis demonstrated mice 1 4 weeks after TAC had cardiac hypertrophy failure, respectively. Cardiac...
Apoptosis may contribute to the development of heart failure, but role apoptotic signaling initiated by endoplasmic reticulum in this condition has not been well clarified.In myocardial samples from patients with quantitative real-time polymerase chain reaction revealed an increase messenger RNA for C/EBP homologous protein (CHOP), a transcriptional factor that mediates reticulum-initiated cell death. We performed transverse aortic constriction or sham operation on wild-type (WT) and...
Significance We identified hypoxia-inducible domain family, member 1A (Higd1a) as a positive regulator of cytochrome c oxidase (CcO). CcO, the terminal component mitochondrial electron transfer system, reductively converts molecular oxygen to water coupled pump protons across inner membrane. Higd1a is transiently induced under hypoxic conditions and increases CcO activity by directly interacting with in vicinity its active center. Induction leads increased consumption subsequent ATP...
Proteasome inhibitors are a novel class of anticancer agents that induce tumour cell death via endoplasmic reticulum (ER) stress. Since ER stress is involved in the development heart failure, we investigated role ER-initiated cardiomyocyte by proteasome inhibition.Rat neonatal cardiomyocytes were used this study. activity was assayed using peptidase substrates. Cell viability and apoptosis measured 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide flow cytometry, respectively....
Proteasomal degradation is altered in many disease phenotypes including cardiac hypertrophy, a prevalent condition leading to heart failure. Our recent investigations identified heterogeneous subpopulations of proteasome complexes the and implicated multiple mechanisms for their regulation.The study aimed at identification molecular changing function hypertrophic heart.Proteasome function, expression, assembly were analyzed during development hypertrophy induced by β-adrenergic stimulation....
We have previously reported that the prolonged transient acidosis during early reperfusion mediates cardioprotective effects in canine hearts. Recently, postconditioning has been shown to be one of novel strategies mediate cardioprotection. tested contribution cardioprotection postconditioning. Open-chest anesthetized dogs subjected 90-min occlusion left anterior descending coronary artery and 6-h were divided into four groups: 1) control group; no intervention after (n = 6); 2) (Postcon)...
Abstract Augmented AMP-activated protein kinase (AMPK) activity inhibits cell migration, possibly contributing to the clinical benefits of chemical AMPK activators in preventing atherosclerosis, vascular remodelling and cancer metastasis. However, underlying mechanisms remain largely unknown. Here we identify PDZ LIM domain 5 (Pdlim5) as a novel substrate show that it plays critical role inhibition migration. directly phosphorylates Pdlim5 at Ser177. Exogenous expression phosphomimetic...
Significance We developed a sensitive method to assess the activity of oxidative phosphorylation in living cells using FRET-based ATP biosensor. then revealed that G0/G1 switch gene 2, protein rapidly induced by hypoxia, increases mitochondrial production interacting with F o 1 -ATP synthase and protects from critical energy crisis.
Inhibition of cGMP-specific phosphodiesterase 5 (PDE5) ameliorates pathological cardiac remodeling and has been gaining attention as a potential therapy for heart failure. Despite promising results in males, the efficacy PDE5 inhibitor sildenafil female pathologies not determined might be affected by estrogen levels, given hormone's involvement cGMP synthesis. Here, we that heart-protective effect mice depends on presence via mechanism involves myocyte eNOS-dependent synthesis cGMP-dependent...
Bradyarrhythmia is a common clinical manifestation. Although the majority of cases are acquired, genetic analysis families with bradyarrhythmia has identified growing number causative gene mutations. Because only ultimate treatment for symptomatic been invasive surgical implantation pacemaker, discovery novel therapeutic molecular targets necessary to improve prognosis and quality life.We investigated family containing 7 individuals autosomal dominant bradyarrhythmias sinus node dysfunction,...
Cardiac-specific myosin light chain kinase (cMLCK), encoded by
We and others have reported that transient accumulation of cyclic AMP (cAMP) in the myocardium during ischemic preconditioning (IP) limits infarct size independent protein kinase C (PKC). Accumulation cAMP activates A (PKA), which has been demonstrated to cause reversible inhibition RhoA Rho-kinase. investigated involvement PKA Rho-kinase limitation by IP.Dogs were subjected 90-minute ischemia 6-hour reperfusion. examined effect on activity sustained (1) preischemic coronary occlusion (IP),...
Background— Adenosine inhibits proliferation of cardiac fibroblasts and hypertrophy cardiomyocytes, both which may play crucial roles in remodeling. In the present study, we investigated whether chronic stimulation adenosine receptors begun after myocardial infarction (MI) prevents Methods Results— MI was produced Wistar rats by permanent ligation left anterior descending coronary artery. One week onset MI, animals were randomized into 8 groups: vehicle, dipyridamole (DIP; uptake inhibitor,...
Land‐atmosphere interactions on the Tibetan Plateau are important because of their influence energy and water cycles both regional global scales. Flux variance eddy covariance methods were used to measure turbulent fluxes heat, vapor, momentum over a shortgrass prairie during Global Energy Water Cycle Experiment (GEWEX) Asian Monsoon (GAME) in 1998. Under unstable conditions monsoon period (July–September), observed standard deviations temperature specific humidity (normalized by appropriate...
Because of their mechanical strength, chemical stability and low molecular weight, carbon nanotubes (CNTs) are attractive biological implant materials. Biomaterials typically implanted into subcutaneous tissue or bone; however, the long-term biopersistence CNTs in these tissues is unknown. Here, tangled oxidized multi-walled (t-ox-MWCNTs) were rat structural changes t-ox-MWCNTs located inside outside macrophages studied for 2 years post-implantation. The majority large agglomerates present...
Dilated cardiomyopathy (DCM) is a major cause of heart failure, characterized by ventricular dilatation and systolic dysfunction. Familial DCM reportedly caused mutations in more than 50 genes, requiring precise disease stratification based on genetic information. However, the underlying causes 60 to 80% familial cases remain unknown. Here, we identified that homozygous truncating gene encoding Bcl-2–associated athanogene (BAG) co-chaperone 5 (BAG5) inherited five patients among four...