A5101836006- Erythrocyte Function and Pathophysiology
- Monoclonal and Polyclonal Antibodies Research
- Blood groups and transfusion
- Glycosylation and Glycoproteins Research
- Advanced Polymer Synthesis and Characterization
- Blood properties and coagulation
- Protein purification and stability
- Hemoglobin structure and function
- Protease and Inhibitor Mechanisms
- Enzyme Structure and Function
- Hydraulic flow and structures
- Surfactants and Colloidal Systems
- Hydrology and Sediment Transport Processes
- Complement system in diseases
- Analytical Chemistry and Chromatography
- Flood Risk Assessment and Management
- Pancreatic function and diabetes
- Coastal and Marine Dynamics
- Blood Coagulation and Thrombosis Mechanisms
- Immune Cell Function and Interaction
- Malaria Research and Control
- Cardiac Imaging and Diagnostics
- Oil Spill Detection and Mitigation
- Neonatal Health and Biochemistry
- Protein Structure and Dynamics
Hamilton Health Sciences
2024
Radboud University Medical Center
2024
St Mary's Hospital
2024
Radboud University Nijmegen
2024
Population Health Research Institute
2024
Sorbonne Université
2024
Artelia (France)
2013-2023
Inserm
2007-2022
Université Paris Cité
1976-2022
Biologie Intégrée du Globule Rouge
2020-2022
Malaria therapy, experimental, and epidemiological studies have shown that erythrocyte Duffy blood group-negative people, largely of African ancestry, are resistant to Plasmodium vivax infection. These findings established a paradigm the antigen is required for P. invasion. endemic in Madagascar, where admixture Duffy-negative Duffy-positive populations diverse ethnic backgrounds has occurred over 2 millennia. There, we investigated susceptibility blood-stage infection disease association...
Photo-responsive polymers are able to change their structure, conformation and properties upon light irradiation.
Background The transition from enucleated reticulocytes to mature normocytes is marked by substantial remodeling of the erythrocytic cytoplasm and membrane. Despite conspicuous changes, most studies describe maturing reticulocyte as a homogenous erythropoietic cell type. While staging based on fluorescent RNA stains such thiazole orange have been useful in clinical setting; these 'sub-vital' may confound delicate biology preclude their use heamoparasite invasion studies. Design Methods Here...
BackgroundMineralocorticoid receptor antagonists have been shown to reduce mortality in patients after myocardial infarction with congestive heart failure. Whether routine use of spironolactone is beneficial uncertain.MethodsIn this multicenter trial a 2-by-2 factorial design, we randomly assigned who had undergone percutaneous coronary intervention receive either or placebo and colchicine placebo. The results the are reported here. two primary outcomes were composite death from...
Rhesus (Rh) antigens are carried by a membrane complex that includes Rh proteins (D and CcEe), Rh-associated glycoproteins (RhAG), accessory chains (LW CD47) associated noncovalent bonds. In heterologous expression systems, RhAG its kidney orthologs function as ammonium transporters. red blood cells (RBCs), it is generally accepted NH 3 permeates lipid diffusion. We have revisited these issues studying RBC ghosts from human mouse genetic variants with defects of comprise the complex. both...
ABSTRACT. Two non-erythroid members of the erythrocyte Rhesus (Rh) protein family, RhBG and RhCG, have been recently cloned in kidney. These proteins share homologies with specific NH3/NH4+ transporters (Mep/Amt) primitive organisms plants. When expressed a Mep-deficient yeast, RhCG can function as bidirectional transporter. The aim this study was to determine intrarenal intracellular location rat RT-PCR on microdissected nephron segments demonstrated expression mRNAs encoding distal...
The ABO blood group influences susceptibility to severe Plasmodium falciparum malaria. Recent evidence indicates that the protective effect of O operates by virtue reduced rosetting infected red cells (iRBCs) with uninfected RBCs. Rosetting is mediated a subgroup PfEMP1 adhesins, RBC binding being assigned N-terminal DBL1α₁ domain. Here, we identify as main receptor for VarO rosetting, marked preference A over B, which in turn preferred We show recombinant NTS-DBL1α₁ and NTS-DBL1α₁-CIDR1γ...
Abstract The light‐responsive behavior in solution and thin films of block copolymers bearing 2‐nitrobenzyl photocleavable esters as side groups is discussed this article. polymers were synthesized by grafting moieties onto poly(acrylic acid)‐ ‐polystyrene (PAA‐ b ‐PS) precursor polymers, leading to poly(2‐nitrobenzyl acrylate‐ random ‐acrylic (P(NBA‐ r ‐AA)‐ copolymers. UV irradiation the a selective solvent for PS led formation micelles that used trap hydrophilic molecules inside their...
Background Rh glycoproteins (RhAG, RhBG, RhCG) are members of the Amt/Mep/Rh family which facilitate movement ammonium across plasma membranes. Changes in transport activity following expression have been described different heterologous systems such as yeasts, oocytes and eukaryotic cell lines. However, these complex systems, a potential contribution endogenous proteins to this function cannot be excluded. To demonstrate that by themselves NH3, human RhCG was purified homogeneity...
The light-induced formation of pH and temperature-responsive poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) nanocages is demonstrated here. strategy based on the self-assembly in aqueous solutions a photocleavable poly(tert-butyl acrylate)-hv-poly[2-(dimethylamino)ethyl block copolymer (PtBA-hv-PDMAEMA, where -hv- junction) into spherical micelles, followed by first PDMAEMA cross-linking bis-iodo compound then UV light irradiation. exposure to induces cleavage junction between PtBA core...
This contribution describes the synthesis and associating behavior in water of a multiresponsive amphiphilic diblock copolymer. copolymer is composed an hydrophobic photocleavable poly(para-methoxyphenacyl methacrylate) block (PMPMA) hydrophilic thermosensitive poly[(oligo ethylene glycol)methacrylate] (POEGMA). The PMPMA-b-POEGMA forms micelles with PMPMA core POEGMA corona water. Light irradiation leads to transformation into poly(methacrylic acid) (PMAA) disruption initial micelles....
The synthesis of photocleavable poly(dimethoxynitrobenzyl acrylate)-block-polystyrene block copolymers is described. UV irradiation these copolymers, dissolved in a good solvent for both blocks, selectively cleaves the dimethoxynitrobenzyl protecting groups, leading to carboxylic acid moieties. Since resulting hydrophilic poly(acrylic acid) insoluble used, self-assembly diblocks into micelles observed. This light-induced micellization process further used trap dyes core micelles.
The invasion of CD71+ reticulocytes by Plasmodium vivax is a crucial yet poorly characterised event. application flow cytometry to ex vivo assays promises facilitate the quantitative analysis P. reticulocyte invasion. However, current protocols suffer from low level sensitivity due absence particular design for cell tropism. Importantly, merozoite into contaminating red blood cells schizont inoculum (auto-invasion) may confound analysis. Here we present stable two-color assay accurate...
Antigen binding by antibodies requires precise orientation of the complementarity- determining region (CDR) loops in variable domain to establish correct contact surface. Members family Camelidae have a modified form immunoglobulin gamma (IgG) with only heavy chains, called Heavy Chain Antibodies (HCAb). HCAbs is mediated three CDR from single (V H H) at N-terminus each chain. This feature V H, along their other important features, e.g., easy expression, small size, thermo-stability and...
Research on stimuli responsive polymers builds momentum as nature-inspired applications using man-made materials are increasingly sought.
The synthesis of poly-acrylates, methacrylates and styrene derivatives by SET-LRP is reported in a user environmentally friendly “green” solvent, dimethyl lactamide (DML).
The Kx protein is an erythrocyte membrane polypeptide which deficient in rare individuals suffering from the McLeod syndrome. gene encoding this has been recently cloned and independently purified as a covalent complex with Kell blood group protein. To further study protein, antisera raised rabbits against six synthetic peptides derived primary sequence of were characterized. All but two precipitated recombinant synthesized coupled transcription-translation vitro. Three reacted on...